Výsledky vyhledávání

Intracellular distribution of kinesin in chromaffin cells

Autor: F, Schmitz, K T, Wallis, M, Rho, D, Drenckhahn, D B, Murphy

Publikováno v: European journal of cell biology. 63(1)

In this paper we examined the association of the microtubule motor protein kinesin with organelles in chromaffin cells. Approximately 15% of kinesin was associated with membranes as determined by differential and equilibrium centrifugation on sucrose

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::c92798fcd0a6e236b2eb030524f4c664
https://pubmed.ncbi.nlm.nih.gov/8005108

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The mechanism of equilibrium binding of microtubule-associated protein 2 to microtubules. Binding is a multi-phasic process and exhibits positive cooperativity

Autor: K T, Wallis, S, Azhar, M B, Rho, S A, Lewis, N J, Cowan, D B, Murphy

Publikováno v: The Journal of biological chemistry. 268(20)

The mechanism of binding of microtubule-associated protein 2 (MAP2) to taxol-stabilized microtubules (MTs) was examined through Scatchard analysis of equilibrium binding and by immunoelectron microscopy. We demonstrate the following. 1) Binding is a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::0f4d958dc53630871867099820d552d9
https://pubmed.ncbi.nlm.nih.gov/8100819

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Identity and origin of the ATPase activity associated with neuronal microtubules. II. Identification of a 50,000-dalton polypeptide with ATPase activity similar to F-1 ATPase from mitochondria

Autor: R R Hiebsch, K T Wallis, D B Murphy

Publikováno v: The Journal of Cell Biology

We determined that the ATPase activity contained in preparations of neuronal microtubules is associated with a 50,000-dalton polypeptide by four different methods: (a) photoaffinity labeling of the pelletable ATPase fraction with [gamma-32P]-8-azido-

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1b8b0ee3afa21b0c20d456eed1e610b6
http://europepmc.org/articles/PMC2112650

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Brain and erythrocyte microtubules from chicken contain different beta-tubulin polypeptides

Autor: D B Murphy, K T Wallis

Publikováno v: Journal of Biological Chemistry. 258:7870-7875

beta-Tubulin subunits isolated from chicken brain tissue and erythrocytes are distinguishable as unique biochemical species by electrophoretic and peptide mapping procedures. 1) The subunits of beta-tubulin exhibit major differences in electrophoreti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::2083a6a037342809cc74c51e293d9968
https://doi.org/10.1016/s0021-9258(18)32259-2

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Erythrocyte microtubule assembly in vitro. Tubulin oligomers limit the rate of microtubule self-assembly

Autor: K T Wallis, D B Murphy

Publikováno v: Journal of Biological Chemistry. 261:2319-2324

Chicken erythrocyte tubulin containing a unique beta tubulin variant polymerizes with greater efficiency (lower critical concentration) but at a slower rate than chicken brain tubulin. In a previous study we demonstrated that the low net rate of asse

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::bb89ce3e595401b0722d69fb4c89c9ee
https://doi.org/10.1016/s0021-9258(17)35938-0

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Erythrocyte microtubule assembly in vitro. Determination of the effects of erythrocyte tau, tubulin isoforms, and tubulin oligomers on erythrocyte tubulin assembly, and comparison with brain microtubule assembly

Autor: K T Wallis, D B Murphy

Publikováno v: Journal of Biological Chemistry. 260:12293-12301

Two tubulin variants, isolated from chicken brain and erythrocytes and known to have different peptide maps and electrophoretic properties, are demonstrated to exhibit different assembly properties in vitro: 1) erythrocyte tubulin assembles with grea

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::fce1407a5ee4a657852beeca69d15157
https://doi.org/10.1016/s0021-9258(17)39024-5

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Isolation of microtubule protein from chicken erythrocytes and determination of the critical concentration for tubulin polymerization in vitro and in vivo

Autor: K T Wallis, D B Murphy

Publikováno v: Journal of Biological Chemistry. 258:8357-8364

Microtubule protein isolated from nucleated chicken erythrocytes was examined with respect to composition and assembly properties to determine its significance in a microtubule bundle called the marginal band. 1) The protein contains greater than 95%

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::3606fcb585dbd2bad3baf6498dc860e0
https://doi.org/10.1016/s0021-9258(20)82073-0

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Identity and Origin of the ATPase activity associated with neuronal microtubules. I. The ATPase activity is associated with membrane vesicles

Autor: D B Murphy, K T Wallis, R R Hiebsch

Publikováno v: The Journal of Cell Biology

Microtubule protein purified from brain tissue by cycles of in vitro assembly-disassembly contains ATPase activity that has been postulated to be associated with microtubule-associated proteins (MAPs) and therefore significant for studies of microtub

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1b58f1412381e8f5812ade2f0882d9bb
http://europepmc.org/articles/PMC2112664

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