Cellular state landscape and herpes simplex virus type 1 infection progression are connected

Autor: Maija K. Pietilä, Jana J. Bachmann, Janne Ravantti, Lucas Pelkmans, Cornel Fraefel

Publikováno v: Nature Communications, Vol 14, Iss 1, Pp 1-20 (2023)

Abstract Prediction, prevention and treatment of virus infections require understanding of cell-to-cell variability that leads to heterogenous disease outcomes, but the source of this heterogeneity has yet to be clarified. To study the multimodal res

Externí odkaz: https://doaj.org/article/52f9a97fca50424ab7fd6e2d51df7975

Archaeal Haloarcula californiae Icosahedral Virus 1 Highlights Conserved Elements in Icosahedral Membrane-Containing DNA Viruses from Extreme Environments

Autor: Tatiana A. Demina, Maija K. Pietilä, Julija Svirskaitė, Janne J. Ravantti, Nina S. Atanasova, Dennis H. Bamford, Hanna M. Oksanen

Publikováno v: mBio, Vol 7, Iss 4 (2016)

ABSTRACT Despite their high genomic diversity, all known viruses are structurally constrained to a limited number of virion morphotypes. One morphotype of viruses infecting bacteria, archaea, and eukaryotes is the tailless icosahedral morphotype with

Externí odkaz: https://doaj.org/article/4bb4fc215536488182b7321277d7f255

Polyprotein Processing as a Determinant for in Vitro Activity of Semliki Forest Virus Replicase

Autor: Maija K. Pietilä, Irina C. Albulescu, Martijn J. van Hemert, Tero Ahola

Publikováno v: Viruses, Vol 9, Iss 10, p 292 (2017)

Semliki Forest virus (SFV) is an arthropod-borne alphavirus that induces membrane invaginations (spherules) in host cells. These harbor the viral replication complexes (RC) that synthesize viral RNA. Alphaviruses have four replicase or nonstructural

Externí odkaz: https://doaj.org/article/a8011f77729d4f57ab89b2f20ec3bbe9

Towards in cellulo virus crystallography

Autor: Jonathan M. Grimes, Helen M. E. Duyvesteyn, Johan Hattne, Gwyndaf Evans, Maija K. Pietilä, Armin Wagner, Aaron S. Brewster, Nicholas K. Sauter, David I. Stuart, Juha T. Huiskonen, Marie-Laure Parsy, Helen M. Ginn, Dennis H. Bamford, Elina A. M. Hirvonen, Geoff Sutton

Publikováno v: Duyvesteyn, HME; Ginn, HM; Pietilä, MK; Wagner, A; Hattne, J; Grimes, JM; et al.(2018). Towards in cellulo virus crystallography. Scientific Reports, 8(1). doi: 10.1038/s41598-018-21693-3. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/9qz3p0v6
Scientific reports, vol 8, iss 1
Scientific Reports, Vol 8, Iss 1, Pp 1-7 (2018)
Scientific Reports

Viruses are a significant threat to both human health and the economy, and there is an urgent need for novel anti-viral drugs and vaccines. High-resolution viral structures inform our understanding of the virosphere, and inspire novel therapies. Here

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::29fd9f41d08a42dcac428496e39ea973
http://www.escholarship.org/uc/item/9qz3p0v6

Purification of Highly Active Alphavirus Replication Complexes Demonstrates Altered Fractionation of Multiple Cellular Membranes

Autor: Maija K. Pietilä, Martijn J. van Hemert, Tero Ahola

Publikováno v: Journal of Virology
Journal of Virology, 92(8)

Positive-strand RNA viruses replicate their genomes in membrane-associated structures; alphaviruses and many other groups induce membrane invaginations called spherules. Here, we established a protocol to purify these membranous replication complexes

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::33aa689e21981a0ffec9a3eb06e8b553
https://doi.org/10.1128/jvi.01852-17