Two alternative conformations of mRNA in the human ribosome during elongation and termination of translation as revealed by EPR spectroscopy

Autor: Ludmila Frolova, Elena G. Bagryanskaya, Olesya A. Krumkacheva, K. N. Bulygin, Galina G. Karpova, Dmitri Graifer, Ivan O. Timofeev, Alexey A. Malygin, Maria I. Meschaninova, Matvey V. Fedin, Alya G. Venyaminova

Publikováno v: Computational and Structural Biotechnology Journal
Computational and Structural Biotechnology Journal, Vol 19, Iss, Pp 4702-4710 (2021)

Graphical abstract
Highlights • DEER reveals the conformational variability of mRNA at the certain translation steps. • Elongation and termination complexes exist in 2 conformations in dynamic equilibrium. • The conformations of mRNA in 40

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c04ce6230b845fcd4b931d502f77bd97
https://doi.org/10.1016/j.csbj.2021.08.024

The functional role of the C-terminal tail of the human ribosomal protein uS19

Autor: Alexey A. Malygin, Alexander V. Gopanenko, Galina G. Karpova, Dmitri Graifer, K. N. Bulygin

Publikováno v: Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms. 1863:194490

The eukaryotic ribosomal protein uS19 has a C-terminal tail that is absent in its bacterial homologue. This tail has been shown to be involved in the formation of the decoding site of human ribosomes. We studied here the previously unexplored functio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4b95ab9df8ccdc454c6466e51e94e2bb
https://doi.org/10.1016/j.bbagrm.2020.194490

Exploring contacts of eRF1 with the 3′-terminus of the P site tRNA and mRNA stop signal in the human ribosome at various translation termination steps

Autor: Ludmila Frolova, K. N. Bulygin, Galina G. Karpova, Dmitri Graifer, Codjo Hountondji

Publikováno v: Biochimica et Biophysica Acta-Gene Regulatory Mechanisms
Biochimica et Biophysica Acta-Gene Regulatory Mechanisms, Elsevier, 2017, 1860 (7), pp.782-793. ⟨10.1016/j.bbagrm.2017.04.004⟩

International audience; Here we employed site-directed cross-linking with the application of tRNA and mRNA analogues bearing an oxidized ribose at the 3′-terminus to investigate mutual arrangement of the main components of translation termination c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::756993a24068dfcb3161bbb999c95c98
https://hal.sorbonne-universite.fr/hal-01906422

The C-terminal fragment of ribosomal protein S15 is located in the decoding site of the human ribosome

Autor: Yu. S. Khairulina, M. V. Molotkov, K. N. Bulygin, Dmitri Graifer, Galina G. Karpova, Aliya G. Venyaminova

Publikováno v: Molecular Biology. 42:270-276

Protein S15 is a characteristic component of the mammalian 80S ribosome that neighbors the mRNA codon at the decoding site and the downstream triplets. The S15 fragment juxtaposed in the human ribosome to mRNA nucleotides +4 to +12 relative to the fi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::250c19c003fedf187eb772258c83cf22
https://doi.org/10.1134/s0026893308020131

Protein S3 in the human 80S ribosome adjoins mRNA 3′ of the A-site codon

Autor: E. A. Popugaeva, Dmitri Graifer, M. V. Molotkov, Aliya G. Venyaminova, Galina G. Karpova, Mariya I. Meschaninova, K. N. Bulygin

Publikováno v: Russian Journal of Bioorganic Chemistry. 33:399-409

The protein environment of mRNA 3′ of the A-site codon (the decoding site) in the human 80S ribosome was studied using a set of oligoribonucleotide derivatives bearing a UUU triplet at the 5′-end and a perfluoroarylazide group at one of the nucle

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::7e9cd631c4a591ca1788c95c3766b5d2
https://doi.org/10.1134/s106816200704005x