Zobrazeno 1 - 10
of 28
pro vyhledávání: '"K L, Polakoski"'
Autor:
R A, Yazigi, K L, Polakoski
Publikováno v:
Archives of pathologylaboratory medicine. 116(10)
To determine the distribution of cocaine administered to male mice in selected extragenital and genital organs and to investigate its possible binding to sperm.Twenty-seven sexually mature virus-free albino male mice were used in various experiments
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::eaa905a75b3e2fa3b439e910d11fc35e
https://pubmed.ncbi.nlm.nih.gov/1417443
https://pubmed.ncbi.nlm.nih.gov/1417443
Publikováno v:
JAMA. 266(14)
Exposure of males to cocaine has been linked to abnormal development of their offspring. To investigate the possible role of sperm, this study examined the interaction of cocaine with human spermatozoa. Washed sperm were incubated with tritiated coca
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::57408839e09d68bc9aeba63cb9d83a79
https://pubmed.ncbi.nlm.nih.gov/1895473
https://pubmed.ncbi.nlm.nih.gov/1895473
Publikováno v:
Biology of reproduction. 44(2)
A cell-free translation system was used to determine the molecular mass of the protein component of precursor(s) to boar proacrosin. Poly(A)(+)-mRNA was extracted from freshly excised boar testis into phenol/chloroform, precipitated in chilled (-20 d
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::907e33f6ec4a90c57a1c245884164a33
https://pubmed.ncbi.nlm.nih.gov/1672614
https://pubmed.ncbi.nlm.nih.gov/1672614
Publikováno v:
Biology of reproduction. 42(2)
Proteinase inhibitors have been shown to be capable of preventing various aspects of fertilization. Diisopropyl fluorophosphate (DFP) is an irreversible inhibitor of trypsin-like enzymes that is commercially available in a radiolabeled form. The expe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::5a201900b8a6e7d9bb3fd883963a8a03
https://pubmed.ncbi.nlm.nih.gov/2110839
https://pubmed.ncbi.nlm.nih.gov/2110839
Publikováno v:
Journal of Biological Chemistry. 257:3095-3099
A proacrosin conversion inhibitor present in boar spermatozoa has been purified and initially characterized. Purification methods included sequential acid extractions of washed spermatozoa at pH 4.0, pH 3.5, and pH 2.5 followed by successive gel filt
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3ec02935a9a723c11183eb7807ccaee0
https://doi.org/10.1016/s0021-9258(19)81078-5
https://doi.org/10.1016/s0021-9258(19)81078-5
Autor:
K L Polakoski, J W Straus
Publikováno v:
Biochemical Journal. 201:657-660
Proacrosin, the zymogen precursor of acrosin, was shown to associate with anionic phospholipid membranes through apparent electrostatic charge interactions. This association was diminished by elevated cation concentrations and was dependent on membra
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::12467a3b9b36037d9115228a24fcc617
https://doi.org/10.1042/bj2010657
https://doi.org/10.1042/bj2010657
Publikováno v:
Journal of Biological Chemistry. 256:5662-5668
Acrosin, an enzyme required for fertilization, is an endogenous proteinase associated with membranes of the sperm acrosome. Liposomes were utilized as a model system to evaluate the mode of association between highly purified boar acrosin and phospho
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::740c3678bbd8c9a07e63133dd1bad4c3
https://doi.org/10.1016/s0021-9258(19)69256-2
https://doi.org/10.1016/s0021-9258(19)69256-2
Autor:
K L Polakoski, R F Parrish
Publikováno v:
Journal of Biological Chemistry. 253:8428-8432
The preparation of highly purified malpha-acrosin is described. Purification was achieved by controlled activation of partially purified proacrosin, followed by gel chromatography over Sephadex G-100 at pH 3.0. The final malpha-acrosin preparation re
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d43ae477099c6d129b20cc6f6703fd7a
https://doi.org/10.1016/s0021-9258(17)34309-0
https://doi.org/10.1016/s0021-9258(17)34309-0
Autor:
R F Parrish, K L Polakoski
Publikováno v:
Journal of Biological Chemistry. 252:1888-1894
Two forms of proacrosin have been purified from ejacualted boar spermatozoa. The isolation method utilized benzamidine to inhibit the premeture activation of the zymogen and included pH precipitation, ammonium sulfate fractionation, and sodium chlori
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::19ce25a6aec73048619beb2e99b616a8
https://doi.org/10.1016/s0021-9258(18)71841-3
https://doi.org/10.1016/s0021-9258(18)71841-3
Autor:
K. L. Polakoski, R. F. Parrish
Publikováno v:
Reproduction. 62:417-422
The in-vitro proteolytic activity of boar sperm acrosin was stimulated by a series of monovalent and divalent metal ions. Equivalent concentrations of monovalent cations resulted in nearly identical increases in proteolytic activity, probably related
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0640dcb33180985511e74a6444a4616b
https://doi.org/10.1530/jrf.0.0620417
https://doi.org/10.1530/jrf.0.0620417