Identification of subunit-subunit interaction sites in αA-WT crystallin and mutant αA-G98R crystallin using isotope-labeled cross-linker and mass spectrometry.

Autor: Rama Kannan, Puttur Santhoshkumar, Brian P Mooney, K Krishna Sharma

Publikováno v: PLoS ONE, Vol 8, Iss 6, p e65610 (2013)

Cataract is characterized by progressive protein aggregation and loss of vision. α-Crystallins are the major proteins in the lens responsible for maintaining transparency. They exist in the lens as highly polydisperse oligomers with variable numbers

Externí odkaz: https://doaj.org/article/d2d668099d0140d8a7dae8283047cebd

αA-crystallin peptide SDRDKFVIFLDVKHF accumulating in aging lens impairs the function of α-crystallin and induces lens protein aggregation.

Autor: Puttur Santhoshkumar, Murugesan Raju, K Krishna Sharma

Publikováno v: PLoS ONE, Vol 6, Iss 4, p e19291 (2011)

The eye lens is composed of fiber cells that are filled with α-, β- and γ-crystallins. The primary function of crystallins is to maintain the clarity of the lens through ordered interactions as well as through the chaperone-like function of α-cry

Externí odkaz: https://doaj.org/article/d49a9ed3fed243f59df069aff4435310

Anti-Inflammatory Activity of Human Lens Crystallin Derived Peptide

Autor: Srinivas Mutalik, Tenzin Tender, Raghu Chandrashekar Hariharapura, Anuraag Muralidharan, K. Krishna Sharma, Pallavi K. Shetty

Publikováno v: Current Drug Delivery. 18:1330-1337

Background: Inflammation has become the culmination point for several chronic diseases like skin diseases, asthma, neurological disorders, cancer, and cardiovascular disorders. Mini αA-crystallin peptide, identified from a highly conserved region of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b6cb17a304f2e7041604b850fdd9380
https://doi.org/10.2174/1567201818666210303095120

Failure of Oxysterols Such as Lanosterol to Restore Lens Clarity from Cataracts

Autor: K. Krishna Sharma, Peter F. Kador, Ashutosh S. Phadte, Marjorie F. Lou, Puttur Santhoshkumar, Damian M. Daszynski, Haizhen A. Zhong

Publikováno v: Scientific Reports, Vol 9, Iss 1, Pp 1-14 (2019)
Scientific Reports

The paradigm that cataracts are irreversible and that vision from cataracts can only be restored through surgery has recently been challenged by reports that oxysterols such as lanosterol and 25-hydroxycholesterol can restore vision by binding to αB

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7f48cba3f41f04ae1fafb52d4b945629
http://link.springer.com/article/10.1038/s41598-019-44676-4

Effect of Structural Changes Induced by Deletion of 54FLRAPSWF61 Sequence in αB-crystallin on Chaperone Function and Anti-Apoptotic Activity

Autor: Srabani Karmakar, Puttur Santhoshkumar, Sundararajan Mahalingam, K. Krishna Sharma

Publikováno v: International Journal of Molecular Sciences
Volume 22
Issue 19
International Journal of Molecular Sciences, Vol 22, Iss 10771, p 10771 (2021)

Previously, we showed that the removal of the 54–61 residues from αB-crystallin (αBΔ54–61) results in a fifty percent reduction in the oligomeric mass and a ten-fold increase in chaperone-like activity. In this study, we investigated the oligo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::71ac9b426083a70ac4863663e18e4c66
https://doi.org/10.3390/ijms221910771