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A method is described which permits the ligation- mediated PCR amplification of specific fragments from a Class-II restriction endonuclease total digest. Feasibility was tested using Bcl I and phage lambda DNA as a model enzyme and amplicon system, r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee224b468afaf05f4b5af4c998b2a525
https://europepmc.org/articles/PMC146646/
https://europepmc.org/articles/PMC146646/
Publikováno v:
Yeast (Chichester, England). 12(4)
Exogenous fatty acids transcriptionally control the expression of a wide variety of eukaryotic genes, many of which encode proteins involved in lipid metabolism. To identify gene products involved in the lipid signalling pathway, a reporter plasmid c