Zobrazeno 1 - 10
of 485
pro vyhledávání: '"K, Gifford"'
Autor:
Tulsi Patel, Jennifer Hammelman, Siaresh Aziz, Sumin Jang, Michael Closser, Theodore L. Michaels, Jacob A. Blum, David K. Gifford, Hynek Wichterle
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-20 (2022)
Patel et al. show that gene expression and regulation in motor neurons is dynamic until juvenile age. A core subprogram (~40% of genes) is faithfully recapitulated in cultured motor neurons, with neuronal activity playing only a modulatory role.
Externí odkaz:
https://doaj.org/article/8c6a3121fd1145a590671bdfc9c21b17
Autor:
Brandon Carter, Pinghan Huang, Ge Liu, Yuejin Liang, Paulo J. C. Lin, Bi-Hung Peng, Lindsay G. A. McKay, Alexander Dimitrakakis, Jason Hsu, Vivian Tat, Panatda Saenkham-Huntsinger, Jinjin Chen, Clarety Kaseke, Gaurav D. Gaiha, Qiaobing Xu, Anthony Griffiths, Ying K. Tam, Chien-Te K. Tseng, David K. Gifford
Publikováno v:
Frontiers in Immunology, Vol 14 (2023)
Licensed COVID-19 vaccines ameliorate viral infection by inducing production of neutralizing antibodies that bind the SARS-CoV-2 Spike protein and inhibit viral cellular entry. However, the clinical effectiveness of these vaccines is transitory as vi
Externí odkaz:
https://doaj.org/article/37d4703fe4c94f879a346594ee70e087
Autor:
Heysol C. Bermudez-Cabrera, Sannie Culbertson, Sammy Barkal, Benjamin Holmes, Max W. Shen, Sophia Zhang, David K. Gifford, Richard I. Sherwood
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-10 (2021)
The mutational outcome of CRISPR-Cas9 editing can be both predictable and targeted. Here the authors show that ATM inhibitor KU-60019 increases 1 bp insertions at the targeted locus.
Externí odkaz:
https://doaj.org/article/77675116590e4b8aa7e1d10c6a4309b0
Publikováno v:
eLife, Vol 11 (2022)
T cells play a critical role in the adaptive immune response, recognizing peptide antigens presented on the cell surface by major histocompatibility complex (MHC) proteins. While assessing peptides for MHC binding is an important component of probing
Externí odkaz:
https://doaj.org/article/3b3a30405efb40ba9ede9aea6f8d86ee
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
Single-cell RNA-Seq allows us to observe snapshots of how biological systems change over time at cellular resolution. Here, the authors develop a generative framework that uses time-resolved single-cell data to model how cells change in physical time
Externí odkaz:
https://doaj.org/article/197cf2be15834f71b3824cd58fa503a3
Autor:
Vedagopuram Sreekanth, Qingxuan Zhou, Praveen Kokkonda, Heysol C. Bermudez-Cabrera, Donghyun Lim, Benjamin K. Law, Benjamin R. Holmes, Santosh K. Chaudhary, Rajaiah Pergu, Brittany S. Leger, James A. Walker, David K. Gifford, Richard I. Sherwood, Amit Choudhary
Publikováno v:
ACS Central Science, Vol 6, Iss 12, Pp 2228-2237 (2020)
Externí odkaz:
https://doaj.org/article/f59a8cf4db8b44faa234b0cf6a54c506
Autor:
Jennifer Hammelman, David K Gifford
Publikováno v:
PLoS Computational Biology, Vol 17, Iss 8, p e1009282 (2021)
Discovering sequence features that differentially direct cells to alternate fates is key to understanding both cellular development and the consequences of disease related mutations. We introduce Expected Pattern Effect and Differential Expected Patt
Externí odkaz:
https://doaj.org/article/f68a75bb216e4deea942b65eba7350a7
Publikováno v:
BMC Bioinformatics, Vol 20, Iss 1, Pp 1-14 (2019)
Abstract Background Visualization tools for deep learning models typically focus on discovering key input features without considering how such low level features are combined in intermediate layers to make decisions. Moreover, many of these methods
Externí odkaz:
https://doaj.org/article/c64c7d1f60f746bbbe83a3a758dbef28
Autor:
Grace Hui Ting Yeo, Oscar Juez, Qing Chen, Budhaditya Banerjee, Lendy Chu, Max W Shen, May Sabry, Ive Logister, Richard I Sherwood, David K Gifford
Publikováno v:
PLoS Computational Biology, Vol 17, Iss 3, p e1008789 (2021)
We introduce poly-adenine CRISPR gRNA-based single-cell RNA-sequencing (pAC-Seq), a method that enables the direct observation of guide RNAs (gRNAs) in scRNA-seq. We use pAC-Seq to assess the phenotypic consequences of CRISPR/Cas9 based alterations o
Externí odkaz:
https://doaj.org/article/caf6d122ce4e4d598a6d62fc160301d9
Autor:
Wilson Louie, Max W Shen, Zakir Tahiry, Sophia Zhang, Daniel Worstell, Christopher A Cassa, Richard I Sherwood, David K Gifford
Publikováno v:
PLoS Computational Biology, Vol 17, Iss 1, p e1008605 (2021)
Restoring gene function by the induced skipping of deleterious exons has been shown to be effective for treating genetic disorders. However, many of the clinically successful therapies for exon skipping are transient oligonucleotide-based treatments
Externí odkaz:
https://doaj.org/article/d7839036eeb942be9da582fbcf3a7ab6