Zobrazeno 1 - 6
of 6
pro vyhledávání: '"Justine Turlin"'
Publikováno v:
mSystems, Vol 8, Iss 3 (2023)
ABSTRACT The soil bacterium Pseudomonas putida is a robust biomanufacturing host that assimilates a broad range of substrates while efficiently coping with adverse environmental conditions. P. putida is equipped with functions related to one-carbon (
Externí odkaz:
https://doaj.org/article/cd1f0d54c1ac42c3930740990e16029a
Publikováno v:
Microbial Biotechnology, Vol 13, Iss 1, Pp 222-232 (2020)
Summary Most of the gene expression systems available for Gram‐negative bacteria are afflicted by relatively high levels of basal (i.e. leaky) expression of the target gene(s). This occurrence affects the system dynamics, ultimately reducing the ou
Externí odkaz:
https://doaj.org/article/79c7b8249338463d8bd1a2016b4e2af3
Publikováno v:
Metabolic Engineering Communications, Vol 10, Iss , Pp e00126- (2020)
Genome engineering of non-conventional microorganisms calls for the development of dedicated synthetic biology tools. Pseudomonas putida is a Gram-negative, non-pathogenic soil bacterium widely used for metabolic engineering owing to its versatile me
Externí odkaz:
https://doaj.org/article/3aae784c5c134f06a988cbc8038efea9
Publikováno v:
Turlin, J, Dronsella, B, De Maria, A, Lindner, S N & Nikel, P I 2022, ' Integrated rational and evolutionary engineering of genome-reduced Pseudomonas putida strains promotes synthetic formate assimilation ', Metabolic Engineering, vol. 74, pp. 191-205 . https://doi.org/10.1016/j.ymben.2022.10.008
Formate is a promising, water-soluble C1 feedstock for biotechnology that can be efficiently produced from CO2—but formatotrophy has been engineered in only a few industrially-relevant microbial hosts. We addressed the challenge of expanding the fe
Publikováno v:
Metabolic Engineering Communications
Volke, D C, Friis, L, Wirth, N T, Turlin, J & Nikel, P I 2020, ' Synthetic control of plasmid replication enables target-and self-curing of vectors and expedites genome engineering of Pseudomonas putida ', Metabolic Engineering Communications, vol. 10, pp. e00126 . https://doi.org/10.1016/j.mec.2020.e00126
Metabolic Engineering Communications, Vol 10, Iss, Pp e00126-(2020)
Volke, D C, Friis, L, Wirth, N T, Turlin, J & Nikel, P I 2020, ' Synthetic control of plasmid replication enables target-and self-curing of vectors and expedites genome engineering of Pseudomonas putida ', Metabolic Engineering Communications, vol. 10, pp. e00126 . https://doi.org/10.1016/j.mec.2020.e00126
Metabolic Engineering Communications, Vol 10, Iss, Pp e00126-(2020)
Genome engineering of non-conventional microorganisms calls for the development of dedicated synthetic biology tools. Pseudomonas putida is a Gram-negative, non-pathogenic soil bacterium widely used for metabolic engineering owing to its versatile me
Publikováno v:
Microbial Biotechnology
Microbial Biotechnology, Vol 13, Iss 1, Pp 222-232 (2020)
Volke, D C, Turlin, J, Mol, V & Nikel, P I 2020, ' Physical decoupling of XylS/Pm regulatory elements and conditional proteolysis enable precise control of gene expression in Pseudomonas putida ', Microbial Biotechnology, vol. 13, no. 1, pp. 222-232 . https://doi.org/10.1111/1751-7915.13383
Microbial Biotechnology, Vol 13, Iss 1, Pp 222-232 (2020)
Volke, D C, Turlin, J, Mol, V & Nikel, P I 2020, ' Physical decoupling of XylS/Pm regulatory elements and conditional proteolysis enable precise control of gene expression in Pseudomonas putida ', Microbial Biotechnology, vol. 13, no. 1, pp. 222-232 . https://doi.org/10.1111/1751-7915.13383
Summary Most of the gene expression systems available for Gram‐negative bacteria are afflicted by relatively high levels of basal (i.e. leaky) expression of the target gene(s). This occurrence affects the system dynamics, ultimately reducing the ou