A suite of PCR-LwCas13a assays for detection and genotyping of Treponema pallidum in clinical samples

Autor: Wentao Chen, Hao Luo, Lihong Zeng, Yuying Pan, Jonathan B. Parr, Yinbo Jiang, Clark H. Cunningham, Kelly L. Hawley, Justin D. Radolf, Wujian Ke, Jiangli Ou, Jianjiang Yang, Bin Yang, Heping Zheng

Publikováno v: Nature Communications, Vol 13, Iss 1, Pp 1-11 (2022)

Clinical diagnosis of Treponema pallidum subspecies pallidum (TPA), the causative agent of syphilis, depends upon serological testing, which has reduced sensitivity for some stages of the disease. Accompanying methods to complement serological testin

Externí odkaz: https://doaj.org/article/7b11fc3cd1da4553bb1b818c106e3ae1

Development of a biomarker signature using grating-coupled fluorescence plasmonic microarray for diagnosis of MIS-C

Autor: Michele Maltz-Matyschsyk, Clare K. Melchiorre, Katherine W. Herbst, Alexander H. Hogan, Kristina Dibble, Brandon O’Sullivan, Joerg Graf, Aishwarya Jadhav, David A. Lawrence, William T. Lee, Kyle J. Carson, Justin D. Radolf, Juan C. Salazar, Michael A. Lynes, Connecticut Children’s COVID Collaborative

Publikováno v: Frontiers in Bioengineering and Biotechnology, Vol 11 (2023)

Multisystem inflammatory syndrome in children (MIS-C) is a rare but serious condition that can develop 4–6 weeks after a school age child becomes infected by SARS-CoV-2. To date, in the United States more than 8,862 cases of MIS-C have been identif

Externí odkaz: https://doaj.org/article/110a005d844644f789de78439dd2827d

Extracellular Loops of the Treponema pallidum FadL Orthologs TP0856 and TP0858 Elicit IgG Antibodies and IgG+-Specific B-Cells in the Rabbit Model of Experimental Syphilis

Autor: Kristina N. Delgado, Jairo M. Montezuma-Rusca, Isabel C. Orbe, Melissa J. Caimano, Carson J. La Vake, Amit Luthra, Christopher M. Hennelly, Fredrick N. Nindo, Jacob W. Meyer, Letitia D. Jones, Jonathan B. Parr, Juan C. Salazar, M. Anthony Moody, Justin D. Radolf, Kelly L. Hawley

Publikováno v: mBio, Vol 13, Iss 4 (2022)

ABSTRACT The resurgence of syphilis in the new millennium has called attention to the importance of a vaccine for global containment strategies. Studies with immune rabbit serum (IRS) indicate that a syphilis vaccine should elicit antibodies (Abs) th

Externí odkaz: https://doaj.org/article/a5028a454e21422fb729d7c1f9cd8a9f

Bacterial Indole as a Multifunctional Regulator of Klebsiella oxytoca Complex Enterotoxicity

Autor: Nagender Ledala, Mishika Malik, Karim Rezaul, Sara Paveglio, Anthony Provatas, Aaron Kiel, Melissa Caimano, Yanjiao Zhou, Jonathan Lindgren, Kristyna Krasulova, Peter Illes, Zdeněk Dvořák, Sandhya Kortagere, Sabine Kienesberger, Amar Cosic, Lisa Pöltl, Ellen L. Zechner, Subho Ghosh, Sridhar Mani, Justin D. Radolf, Adam P. Matson

Publikováno v: mBio, Vol 13, Iss 1 (2022)

ABSTRACT Gastrointestinal microbes respond to biochemical metabolites that coordinate their behaviors. Here, we demonstrate that bacterial indole functions as a multifactorial mitigator of Klebsiella grimontii and Klebsiella oxytoca pathogenicity. Th

Externí odkaz: https://doaj.org/article/fe63cc8ff8ca415a9500fb87f0e2d592

Genomic insights into the Ixodes scapularis tick vector of Lyme disease

Autor: Monika Gulia-Nuss, Andrew B. Nuss, Jason M. Meyer, Daniel E. Sonenshine, R. Michael Roe, Robert M. Waterhouse, David B. Sattelle, José de la Fuente, Jose M. Ribeiro, Karine Megy, Jyothi Thimmapuram, Jason R. Miller, Brian P. Walenz, Sergey Koren, Jessica B. Hostetler, Mathangi Thiagarajan, Vinita S. Joardar, Linda I. Hannick, Shelby Bidwell, Martin P. Hammond, Sarah Young, Qiandong Zeng, Jenica L. Abrudan, Francisca C. Almeida, Nieves Ayllón, Ketaki Bhide, Brooke W. Bissinger, Elena Bonzon-Kulichenko, Steven D. Buckingham, Daniel R. Caffrey, Melissa J. Caimano, Vincent Croset, Timothy Driscoll, Don Gilbert, Joseph J. Gillespie, Gloria I. Giraldo-Calderón, Jeffrey M. Grabowski, David Jiang, Sayed M. S. Khalil, Donghun Kim, Katherine M. Kocan, Juraj Koči, Richard J. Kuhn, Timothy J. Kurtti, Kristin Lees, Emma G. Lang, Ryan C. Kennedy, Hyeogsun Kwon, Rushika Perera, Yumin Qi, Justin D. Radolf, Joyce M. Sakamoto, Alejandro Sánchez-Gracia, Maiara S. Severo, Neal Silverman, Ladislav Šimo, Marta Tojo, Cristian Tornador, Janice P. Van Zee, Jesús Vázquez, Filipe G. Vieira, Margarita Villar, Adam R. Wespiser, Yunlong Yang, Jiwei Zhu, Peter Arensburger, Patricia V. Pietrantonio, Stephen C. Barker, Renfu Shao, Evgeny M. Zdobnov, Frank Hauser, Cornelis J. P. Grimmelikhuijzen, Yoonseong Park, Julio Rozas, Richard Benton, Joao H. F. Pedra, David R. Nelson, Maria F. Unger, Jose M. C. Tubio, Zhijian Tu, Hugh M. Robertson, Martin Shumway, Granger Sutton, Jennifer R. Wortman, Daniel Lawson, Stephen K. Wikel, Vishvanath M. Nene, Claire M. Fraser, Frank H. Collins, Bruce Birren, Karen E. Nelson, Elisabet Caler, Catherine A. Hill

Publikováno v: Nature Communications, Vol 7, Iss 1, Pp 1-13 (2016)

Ticks transmit a large number of pathogens that cause human diseases. Here, the authors sequence the genome of the tick Ixodes scapularisand uncover expansion of genes associated with parasitic processes unique to ticks and tick-host interactions.

Externí odkaz: https://doaj.org/article/fa4043b3779c4d0db50b094e385f7e3b