Zobrazeno 1 - 10
of 21
pro vyhledávání: '"Justin D Vrana"'
Autor:
Justin D Vrana, Nuttada Panpradist, Nikki Higa, Daisy Ko, Parker Ruth, Ruth Kanthula, James J Lai, Yaoyu Yang, Samar R Sakr, Bhavna Chohan, Michael H Chung, Lisa M Frenkel, Barry R Lutz, Eric Klavins, Ingrid A Beck
Publikováno v:
PLOS Global Public Health, Vol 2, Iss 2, p e0000185 (2022)
Usability is an overlooked aspect of implementing lab-based assays, particularly novel assays in low-resource-settings. Esoteric instructions can lead to irreproducible test results and patient harm. To address these issues, we developed a software a
Externí odkaz:
https://doaj.org/article/dcdfb395c1bb4ae6a750cadd407f1b57
Publikováno v:
Nature Communications, Vol 8, Iss 1, Pp 1-11 (2017)
The leakiness of commonly used genetic components can make the construction of complex synthetic circuits difficult. Here the authors construct NOR gate architecture, using dCas9 fused to the chromatin remodeller Mxi1, that can be wired together into
Externí odkaz:
https://doaj.org/article/c217f5f654de4540a39993619de94545
Autor:
Breschine Cummins, Robert C. Moseley, Anastasia Deckard, Mark Weston, George Zheng, Daniel Bryce, Joshua Nowak, Marcio Gameiro, Tomas Gedeon, Konstantin Mischaikow, Jacob Beal, Tessa Johnson, Matthew Vaughn, Niall I. Gaffney, Shweta Gopaulakrishnan, Joshua Urrutia, Robert P. Goldman, Bryan Bartley, Tramy T. Nguyen, Nicholas Roehner, Tom Mitchell, Justin D. Vrana, Katie J. Clowers, Narendra Maheshri, Diveena Becker, Ekaterina Mikhalev, Vanessa Biggers, Trissha R. Higa, Lorraine A. Mosqueda, Steven B. Haase
A challenge in the design and construction of synthetic genetic circuits is that they will operate within biological systems that have noisy and changing parameter regimes that are largely unmeasurable. The outcome is that these circuits do not opera
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::acb350e59a276963ace339cc4381b44a
https://doi.org/10.1101/2022.06.13.495701
https://doi.org/10.1101/2022.06.13.495701
Autor:
Robert P. Goldman, Robert Moseley, Nicholas Roehner, Bree Cummins, Justin D. Vrana, Katie J. Clowers, Daniel Bryce, Jacob Beal, Matthew DeHaven, Joshua Nowak, Trissha Higa, Vanessa Biggers, Peter Lee, Jeremy P. Hunt, Lorraine Mosqueda, Steven B. Haase, Mark Weston, George Zheng, Anastasia Deckard, Shweta Gopaulakrishnan, Joseph F. Stubbs, Niall I. Gaffney, Matthew W. Vaughn, Narendra Maheshri, Ekaterina Mikhalev, Bryan Bartley, Richard Markeloff, Tom Mitchell, Tramy Nguyen, Daniel Sumorok, Nicholas Walczak, Chris Myers, Zach Zundel, Benjamin Hatch, James Scholz, John Colonna-Romano
We describe an experimental campaign that replicated the performance assessment of logic gates engineered into cells of S. cerevisiae by Gander, et al. Our experimental campaign used a novel high throughput experimentation framework developed under D
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3e5a6dae48c1a2cd916514191289c547
https://doi.org/10.1101/2022.05.31.493627
https://doi.org/10.1101/2022.05.31.493627
Autor:
Robert P Goldman, Robert Moseley, Nicholas Roehner, Breschine Cummins, Justin D Vrana, Katie J Clowers, Daniel Bryce, Jacob Beal, Matthew DeHaven, Joshua Nowak, Trissha Higa, Vanessa Biggers, Peter Lee, Jeremy P Hunt, Lorraine Mosqueda, Steven B Haase, Mark Weston, George Zheng, Anastasia Deckard, Shweta Gopaulakrishnan, Joseph F Stubbs, Niall I Gaffney, Matthew W Vaughn, Narendra Maheshri, Ekaterina Mikhalev, Bryan Bartley, Richard Markeloff, Tom Mitchell, Tramy Nguyen, Daniel Sumorok, Nicholas Walczak, Chris Myers, Zach Zundel, Benjamin Hatch, James Scholz, John Colonna-Romano
Publikováno v:
Synthetic Biology. 7
We describe an experimental campaign that replicated the performance assessment of logic gates engineered into cells of Saccharomyces cerevisiae by Gander et al. Our experimental campaign used a novel high-throughput experimentation framework develop
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cb57b78352daf08acb3bbe38f5542387
https://doi.org/10.1093/synbio/ysac018
https://doi.org/10.1093/synbio/ysac018
Autor:
Justin D. Vrana, Parker S. Ruth, Ute Dagmar Feucht, Laddawan Laomanit, Ingrid A. Beck, Annie Wong-On-Wing, Daisy Ko, David McIntyre, Jonathan Lim, Enos Kline, Ross S. Milne, Barry R. Lutz, James J. Lai, Eric Klavins, Nicole Ngo-Giang-Huong, Ruth Kanthula, Nuttada Panpradist, Isaac So, Lisa M. Frenkel, Michael H. Chung, Nikki Higa, Theresa M. Rossouw, Jaime Soria, Gonzague Jourdain
Publikováno v:
EBioMedicine, Vol 50, Iss, Pp 34-44 (2019)
EBioMedicine
EBioMedicine
Background: HIV drug resistance (HIVDR) testing can assist clinicians in selecting treatments. However, high complexity and cost of genotyping assays limit routine testing in settings where HIVDR prevalence has reached high levels. Methods: The oligo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c5c1fdbb283e327dd85f1dd3441b6762
http://www.sciencedirect.com/science/article/pii/S2352396419307431
http://www.sciencedirect.com/science/article/pii/S2352396419307431
Autor:
Bhavna Chohan, Chung Mh, James J. Lai, Justin D. Vrana, Higa N, Sakr, Parker S. Ruth, Yaoyu Yang, Ingrid A. Beck, Barry R. Lutz, Nuttada Panpradist, Lisa M. Frenkel, Eric Klavins, Daisy Ko, Ruth Kanthula
IntroductionUsability is an overlooked aspect of implementing lab-based assays, particularly novel assays in low-resource-settings. Esoteric instructions can lead to irreproducible test results and patient harm. To address these issues, we developed
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b438928ba224d34ca3e5d487a0b38f22
https://doi.org/10.1101/2021.05.06.21256654
https://doi.org/10.1101/2021.05.06.21256654
Autor:
Justin D. Vrana, Lisa M. Frenkel, Parker S. Ruth, Jack H. Kotnik, Qin Wang, Abraham Miller, Samuel W.A. Stewart, Nuttada Panpradist, Peter D Han, Ingrid A. Beck, Barry R. Lutz, Amy Oreskovic, Lea M. Starita
Publikováno v:
EBioMedicine
EBioMedicine, Vol 66, Iss, Pp 103296-(2021)
EBioMedicine, Vol 66, Iss, Pp 103296-(2021)
Detection of SARS-CoV-2 infections is important for treatment, isolation of infected and exposed individuals, and contact tracing. RT-qPCR is the "gold-standard" method to sensitively detect SARS-CoV-2 RNA, but most laboratory-developed RT-qPCR assay
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73cf9fe0e5e0e20266cb8efcb9094d06
http://europepmc.org/articles/PMC8040774
http://europepmc.org/articles/PMC8040774
Autor:
Devin Strickland, Garrett Newman, Eriberto Lopez, Michelle Parks, Abraham Miller, Ayesha Saleem, Samer Halabiya, Justin D. Vrana, Yaoyu Yang, Benjamin J. Keller, Orlando de Lange, Eric Klavins, Sarah Goldberg, Cameron Cordray
Publikováno v:
Synthetic Biology
Automation has been shown to improve the replicability and scalability of biomedical and bioindustrial research. Although the work performed in many labs is repetitive and can be standardized, few academic labs can afford the time and money required
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::80b79b35ba6c9f1c8aab9b9c7c9bd459
https://doi.org/10.1093/synbio/ysab006
https://doi.org/10.1093/synbio/ysab006
Autor:
Samuel W.A. Stewart, Lea M. Starita, Amy Oreskovic, Barry R. Lutz, Ingrid A. Beck, Peter D Han, Lisa M. Frenkel, Nuttada Panpradist, Qin Wang, Jack H. Kotnik, Parker S. Ruth, Justin D. Vrana, Abraham Miller
Publikováno v:
EBioMedicine, Vol 64, Iss, Pp 103236-(2021)
EBioMedicine
EBioMedicine
Background Detection of SARS-CoV-2 infections is important for treatment, isolation of infected and exposed individuals, and contact tracing. RT-qPCR is the “gold-standard” method to sensitively detect SARS-CoV-2 RNA, but most laboratory-develope
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fc78b3f080eabb02bbb00304b54e44ff
https://doi.org/10.1016/j.ebiom.2021.103236
https://doi.org/10.1016/j.ebiom.2021.103236