Reconstruction of the mouse extrahepatic biliary tree using primary human extrahepatic cholangiocyte organoids

Autor: Sampaziotis, F, Justin, AW, Tysoe, OC, Sawiak, S, Cardoso-De-Brito, M, Ortmann, D, Yangou, L, Ross, A, Bargher, J, Bertero, A, Zonneveld, MCF, Pawlowski, M, Madrigal, P, Georgakopoulos, N, Pirmadjid, N, Zilbauer, M, Sinha, S, Hannan, NRF, Vallier, L

The treatment of common bile duct (CBD) disorders, such as biliary atresia or ischemic strictures, is restricted by the lack of biliary tissue from healthy donors suitable for surgical reconstruction. Here we report a new method for the isolation and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::39eba637d9d6c6652e3123ce9bd45371
https://www.repository.cam.ac.uk/handle/1810/264945

Densified collagen tubular grafts for human tissue replacement and disease modelling applications.

Autor: Justin AW; Department of Engineering, University of Cambridge, Trumpington Street, Cambridge CB2 1PZ, UK. Electronic address: awj27@cam.ac.uk., Cammarata F; Department of Engineering, University of Cambridge, Trumpington Street, Cambridge CB2 1PZ, UK., Guy AA; Department of Engineering, University of Cambridge, Trumpington Street, Cambridge CB2 1PZ, UK., Estevez SR; Department of Engineering, University of Cambridge, Trumpington Street, Cambridge CB2 1PZ, UK., Burgess S; Department of Engineering, University of Cambridge, Trumpington Street, Cambridge CB2 1PZ, UK., Davaapil H; Wellcome-Medical Research Council Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre, University of Cambridge, Cambridge CB2 0AW, UK; Department of Medicine, Division of Cardiovascular Medicine, University of Cambridge, Cambridge CB2 0QQ, UK., Stavropoulou-Tatla A; Department of Engineering, University of Cambridge, Trumpington Street, Cambridge CB2 1PZ, UK., Ong J; Department of Engineering, University of Cambridge, Trumpington Street, Cambridge CB2 1PZ, UK; East of England Gastroenterology Speciality Training Program, Cambridge, UK., Jacob AG; Wellcome-Medical Research Council Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre, University of Cambridge, Cambridge CB2 0AW, UK; Department of Biochemistry, University of Cambridge, Downing Site, Tennis Court Road, Cambridge CB2 1QW, UK., Saeb-Parsy K; Department of Surgery, University of Cambridge, and NIHR Cambridge Biomedical Research Centre, Cambridge CB2 0QQ, UK., Sinha S; Department of Medicine, Division of Cardiovascular Medicine, University of Cambridge, Cambridge CB2 0QQ, UK., Markaki AE; Department of Engineering, University of Cambridge, Trumpington Street, Cambridge CB2 1PZ, UK. Electronic address: am253@cam.ac.uk.

Publikováno v: Biomaterials advances [Biomater Adv] 2023 Feb; Vol. 145, pp. 213245. Date of Electronic Publication: 2022 Dec 14.

Functionalisation of a heat-derived and bio-inert albumin hydrogel with extracellular matrix by air plasma treatment.

Autor: Ong J; Department of Engineering, University of Cambridge, Trumpington Street, Cambridge, CB2 1PZ, UK. jo401@cam.ac.uk.; East of England Gastroenterology Speciality Training Program, Fulbourn, Cambridge, CB21 5XB, UK. jo401@cam.ac.uk.; Department of Medicine, National University of Singapore, Kent Ridge Road, Singapore, 119228, Singapore. jo401@cam.ac.uk., Zhao J; Department of Engineering, University of Cambridge, Trumpington Street, Cambridge, CB2 1PZ, UK.; Duke-NUS Medical School, 8 College Road, Singapore, 169857, Singapore., Levy GK; Department of Engineering, University of Cambridge, Trumpington Street, Cambridge, CB2 1PZ, UK., Macdonald J; Department of Engineering, University of Cambridge, Trumpington Street, Cambridge, CB2 1PZ, UK., Justin AW; Department of Engineering, University of Cambridge, Trumpington Street, Cambridge, CB2 1PZ, UK., Markaki AE; Department of Engineering, University of Cambridge, Trumpington Street, Cambridge, CB2 1PZ, UK. am253@cam.ac.uk.

Publikováno v: Scientific reports [Sci Rep] 2020 Jul 24; Vol. 10 (1), pp. 12429. Date of Electronic Publication: 2020 Jul 24.

Albumin-based hydrogels for regenerative engineering and cell transplantation.

Autor: Ong J; Department of Engineering, University of Cambridge, Cambridge, UK.; Gastroenterology Specialty Training Program, East of England Deanery, Cambridge, UK., Zhao J; Department of Engineering, University of Cambridge, Cambridge, UK., Justin AW; Department of Engineering, University of Cambridge, Cambridge, UK., Markaki AE; Department of Engineering, University of Cambridge, Cambridge, UK.

Publikováno v: Biotechnology and bioengineering [Biotechnol Bioeng] 2019 Dec; Vol. 116 (12), pp. 3457-3468. Date of Electronic Publication: 2019 Oct 06.

Isolation and propagation of primary human cholangiocyte organoids for the generation of bioengineered biliary tissue.

Autor: Tysoe OC; Wellcome Trust-Medical Research Council Stem Cell Institute, Cambridge Stem Cell Institute, Anne McLaren Laboratory, Department of Surgery, University of Cambridge, Cambridge, UK.; Department of Surgery, University of Cambridge, Cambridge, UK., Justin AW; Department of Engineering, University of Cambridge, Cambridge, UK., Brevini T; Wellcome Trust-Medical Research Council Stem Cell Institute, Cambridge Stem Cell Institute, Anne McLaren Laboratory, Department of Surgery, University of Cambridge, Cambridge, UK.; Department of Medical Biotechnology and Translational Medicine (BIOMETRA), Università degli Studi di Milano, Milan, Italy., Chen SE; Wellcome Trust-Medical Research Council Stem Cell Institute, Cambridge Stem Cell Institute, Anne McLaren Laboratory, Department of Surgery, University of Cambridge, Cambridge, UK., Mahbubani KT; Department of Surgery, University of Cambridge, Cambridge, UK., Frank AK; Norwegian PSC Research Center, Department of Transplantation Medicine, Division of Surgery, Inflammatory Diseases and Transplantation, Oslo University Hospital, Rikshospitalet, Oslo, Norway.; Research Institute of Internal Medicine, Division of Surgery, Inflammatory Diseases and Transplantation, Oslo University Hospital, Rikshospitalet, Oslo, Norway.; Department of Medicine III, University Hospital Aachen, Aachen, Germany., Zedira H; Wellcome Trust-Medical Research Council Stem Cell Institute, Cambridge Stem Cell Institute, Anne McLaren Laboratory, Department of Surgery, University of Cambridge, Cambridge, UK., Melum E; Norwegian PSC Research Center, Department of Transplantation Medicine, Division of Surgery, Inflammatory Diseases and Transplantation, Oslo University Hospital, Rikshospitalet, Oslo, Norway.; Research Institute of Internal Medicine, Division of Surgery, Inflammatory Diseases and Transplantation, Oslo University Hospital, Rikshospitalet, Oslo, Norway.; Section for Gastroenterology, Department of Transplantation Medicine, Division of Surgery, Inflammatory Diseases and Transplantation, Oslo University Hospital Rikshospitalet, Oslo, Norway.; Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway., Saeb-Parsy K; Department of Surgery, University of Cambridge, Cambridge, UK., Markaki AE; Department of Engineering, University of Cambridge, Cambridge, UK., Vallier L; Wellcome Trust-Medical Research Council Stem Cell Institute, Cambridge Stem Cell Institute, Anne McLaren Laboratory, Department of Surgery, University of Cambridge, Cambridge, UK. lv225@cam.ac.uk.; Department of Surgery, University of Cambridge, Cambridge, UK. lv225@cam.ac.uk., Sampaziotis F; Wellcome Trust-Medical Research Council Stem Cell Institute, Cambridge Stem Cell Institute, Anne McLaren Laboratory, Department of Surgery, University of Cambridge, Cambridge, UK. fs347@cam.ac.uk.; Department of Surgery, University of Cambridge, Cambridge, UK. fs347@cam.ac.uk.; Department of Hepatology, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK. fs347@cam.ac.uk.; Department of Medicine, University of Cambridge, Cambridge, UK. fs347@cam.ac.uk.

Publikováno v: Nature protocols [Nat Protoc] 2019 Jun; Vol. 14 (6), pp. 1884-1925. Date of Electronic Publication: 2019 May 20.