Direct monitoring of biocatalytic deacetylation of amino acid substrates by 1H NMR reveals fine details of substrate specificity

Autor: Silvia De Cesare, Juraj Bella, Lorna Murray, Nicholas Phillip Mulholland, Catherine A McKenna, Dominic J. Campopiano

Publikováno v: De Cesare, S, Mckenna, C A, Mulholland, N, Murray, L, Bella, J & Campopiano, D J 2021, ' Direct monitoring of biocatalytic deacetylation of amino acid substrates by 1 H NMR reveals fine details of substrate specificity ', Organic & Biomolecular chemistry . https://doi.org/10.1039/D1OB00122A

Amino acids are key synthetic building blocks that can be prepared in an enantiopure form by biocatalytic methods. We show that the l-selective ornithine deacetylase ArgE catalyses hydrolysis of a wide-range of N-acyl-amino acid substrates. This acti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::36ca8c0427e36516262469e99317fa26
https://www.pure.ed.ac.uk/ws/files/210968070/20210520_Campopiano_d1ob00122a.pdf

Non-invasive 19F NMR analysis of a protein-templated N-acylhydrazone dynamic combinatorial library

Autor: Juraj Bella, Jue Theresa Wang, Dominic J Campopiano, Alexander Gosta Ekstrom

Publikováno v: Ekstrom, A G, Wang, J T, Bella, J & Campopiano, D J 2018, ' Non-invasive 19F NMR analysis of a protein-templated N-acylhydrazone dynamic combinatorial library. ', Organic & Biomolecular chemistry . https://doi.org/10.1039/C8OB01918E

Dynamic combinatorial chemistry (DCC) is a powerful tool to identify ligands for biological targets. We used 19F NMR as an in situ, non-invasive technique for measuring the composition of a dynamic combinatorial library (DCL) of N-acylhydrazones (NAH

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::11e1e64d6d367c1af53253ef16a2d925
https://www.pure.ed.ac.uk/ws/files/75777546/20181004_Campopiano_Ekstrom_et_al_OBC.pdf

The interaction of Jagged-1 cytoplasmic tail with afadin PDZ domain is local, folding-independent, and tuned by phosphorylation

Autor: Matija Popovic, Paul N. Barlow, Alessandro Pintar, Ventsislav Zlatev, Juraj Bella, Sándor Pongor, Gregor Anderluh, Vesna Hodnik

Publikováno v: Journal of Molecular Recognition. 24:245-253

Jagged-1, one of the five Notch ligands in man, is a membrane-spanning protein made of a large extracellular region and a 125-residue cytoplasmic tail bearing a C-terminal PDZ recognition motif ((1213) RMEYIV(1218) ). Binding of Jagged-1 intracellula

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c25a80b134f310832d688782e7e12ee2
https://doi.org/10.1002/jmr.1042

Diversity in Guanine-Selective DNA Binding Modes for an Organometallic Ruthenium Arene Complex

Autor: Fuyi Wang, Juraj Bella, Peter J. Sadler, John Parkinson, Jingjing Xu, Susan J. Berners-Price, Hong-Ke Liu

Publikováno v: Angewandte Chemie International Edition. 45:8153-8156

Seek and hide! An organometallic ruthenium arene anticancer complex with ruthenium (pink ball) chelated by ethylenediamine (blue) is selective for guanine bases on DNA and can bury the noncoordinated phenyl ring of its arene ligand (yellow) between b

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bc2ff08b0ff3341b9b961f76f8728a6e
https://doi.org/10.1002/anie.200602873

Competitive reactions of a ruthenium arene anticancer complex with histidine, cytochrome c and an oligonucleotide

Autor: Fuyi Wang, John Parkinson, Peter J. Sadler, Juraj Bella

Publikováno v: JBIC Journal of Biological Inorganic Chemistry. 10:147-155

The ruthenium arene anticancer complex [(eta(6)-bip)Ru(en)Cl][PF(6)] (1) (bip is biphenyl, en is ethylenediamine) reacted slowly with the amino acid L-histidine (L-His) in aqueous solution at 310 K. Two L-His adducts of 1 were separated by high-perfo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c33c3030ee12116b2e13c1363dc4cd3
https://doi.org/10.1007/s00775-004-0621-5