Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Junshi Zheng"'
Publikováno v:
BMC Plant Biology, Vol 19, Iss 1, Pp 1-16 (2019)
Abstract Background Stevia rebaudiana produces sweet-tasting steviol glycosides (SGs) in its leaves which can be used as natural sweeteners. Metabolic engineering of Stevia would offer an alternative approach to conventional breeding for enhanced pro
Externí odkaz:
https://doaj.org/article/6fe0bf2e950b4b96a8750f9c24c39bb5
Publikováno v:
Plant Biotechnology Journal
Summary Steviol glycosides (SGs) are extracted from Stevia leaves for use as a natural sweetener. Among SGs, stevioside is most abundant in leaf extracts followed by rebaudioside A (Reb A). However, Reb A is of particular interest because of its swee
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::640496308f53df7a6f803a937d86895f
https://doi.org/10.1111/pbi.13035
https://doi.org/10.1111/pbi.13035
Figure S1. Representative phenotypes of callus on callus induction media. a Calli induced on media containing 1 mg/L BA and 1 mg/L NAA after 6 weeks. b Calli and shoot regenerated on media containing 1 mg/L BA and 1 mg/L IAA after 6 weeks. c Leaf exp
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::227933b6f81d91d6c7263f82f2021b67
Figure S3. Immunoblot analyses showing GFP expression in transgenic lines. Total leaf protein was extracted from, SrDXS1-OE, SrKAH-OE and WT lines and probed with Îą-GFP antibody. Lower panel shows blot after staining with coomassie blue. Extra pan
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2946cf51e8bdf4583dde5c60a1f09358
Figure S7. Monoterpenes extracted from Stevia plants overexpressing SrDXS1 (SrDXS1-OE). a-c α-pinene (a), β-pinene (b), and linalool (c), extracted from the leaves. All measurements are expressed as mean ± SE and statistical analysis was carried o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a171997127e69db5ed4f743f9dc1379
Figure S8. Transcript levels of SrUGT76G1 in SrKAH-overexpression lines (SrKAH-OE). The values are expressed as mean ± SE (n = 3). Student’s t-test was used for the analysis of statistical significance. (PDF 175 kb)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8fa0f90bd4da8bb8447142c76a928c00
Table S1. List of primers used in study. (PDF 182 kb)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::adf5c087d99eecdfc288ae4cab8ec919
Figure S6. Total content of stevioside and Reb A in SrDXS1-OE line #9. Measurements were made on fresh leaves pooled from five individuals. (PDF 176 kb)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::42a207eb234f08b155de1e423697be1c
Figure S2. Representative phenotypes of the regenerated shoots. a Unhealthy looking regenerated shoots with watery and translucent appearance and slight browning. b and c Healthy looking callus with few shoots typical of regenerated shoots under Cond
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::501e20deb6ab5576171a41acc987e739
Figure S5. Relative content of Reb C and dulcoside A detected from the dried leaves of transgenic Stevia. a Amount of Reb C and Dulcoside A relative to wild type (WT) control line in the SrDXS1 overexpressing lines (SrDXS1-OE). b Relative abundance o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::54ece35e4b5a7b3f63a40f9c2845324a