Zobrazeno 1 - 10
of 20
pro vyhledávání: '"Jungjoon K Lee"'
Publikováno v:
PLoS ONE, Vol 19, Iss 8, p e0306254 (2024)
While computational epitope prediction methods have found broad application, their use, specifically in allergy-related contexts, remains relatively less explored. This study benchmarks several publicly available epitope prediction tools, focusing on
Externí odkaz:
https://doaj.org/article/02966c94e9ca45ff8ef2dda5f19b4525
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-13 (2022)
Methods to predict genome-wide off-target activities of prime editors (PEs) are currently lacking. Here the authors report a cell-based assay, TAgmentation of Prime Editor sequencing (TAPE-seq), that provides genome-wide off-target candidates for PEs
Externí odkaz:
https://doaj.org/article/bb4c07dc70ee4360811926671e37bdb4
Autor:
Jungjoon K. Lee, Euihwan Jeong, Joonsun Lee, Minhee Jung, Eunji Shin, Young-hoon Kim, Kangin Lee, Inyoung Jung, Daesik Kim, Seokjoong Kim, Jin-Soo Kim
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-10 (2018)
Undesired off-target effects can hamper the use of CRISPR-Cas9 in therapeutic applications. Here the authors use a directed evolution approach to develop Sniper-Cas9 which combines high specificity with no loss of on-target activity.
Externí odkaz:
https://doaj.org/article/12e41efab7774824b3a582ae5751b26d
Autor:
Young-hoon Kim, Nahye Kim, Ikenna Okafor, Sungchul Choi, Seonwoo Min, Joonsun Lee, Seung-Min Bae, Keunwoo Choi, Janice Choi, Vinayak Harihar, Youngho Kim, Jin-Soo Kim, Benjamin P. Kleinstiver, Jungjoon K. Lee, Taekjip Ha, Hyongbum Henry Kim
Publikováno v:
Nature Chemical Biology.
Although several high-fidelity SpCas9 variants have been reported, it has been observed that this increased specificity is associated with reduced on-target activity, limiting the applications of the high-fidelity variants when efficient genome editi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fc6d9dae292d0c5be30f3cab4e636d2e
https://doi.org/10.1038/s41589-023-01279-5
https://doi.org/10.1038/s41589-023-01279-5
Autor:
Jeonghun Kwon, Minyoung Kim, Woochang Hwang, Anna Jo, Gue-Ho Hwang, Minhee Jung, Un Gi Kim, Gang Cui, Heonseok Kim, Joon-Ho Eom, Junho K. Hur, Junwon Lee, Youngho Kim, Jin-soo Kim, Sangsu Bae, Jungjoon K. Lee
Publikováno v:
Genome Biology. 24
We present a novel genome-wide off-target prediction method named Extru-seq and compare it with cell-based (GUIDE-seq), in vitro (Digenome-seq), and in silico methods using promiscuous guide RNAs with large numbers of valid off-target sites. Extru-se
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::cda75971f1337be58f056843cf132701
https://doi.org/10.1186/s13059-022-02842-4
https://doi.org/10.1186/s13059-022-02842-4
Autor:
Young-hoon Kim, Nahye Kim, Ikenna Okafor, Sungchul Choi, Seonwoo Min, Joonsun Lee, Keunwoo Choi, Janice Choi, Vinayak Harihar, Youngho Kim, Jin-Soo Kim, Jungjoon K. Lee, Taekjip Ha, Hyongbum Henry Kim
Although several high-fidelity SpCas9 variants that have reduced activities at mismatched target sequences have been reported, it has been observed that this increased specificity is associated with reduced on-target activity, limiting the applicatio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::28c07511677842f72aabc038526728dd
https://doi.org/10.1101/2022.12.05.519240
https://doi.org/10.1101/2022.12.05.519240
Autor:
Yanbo Wang, Minhee Jung, Taekjip Ha, Jungjoon K. Lee, Scott Bailey, Digvijay Singh, John Mallon, Ikenna C Okafor, Haobo Wang
Publikováno v:
Nucleic Acids Research
Cas9 has made a wide range of genomic manipulation possible. However, its specificity continues to be a challenge. Non-canonical gRNAs and new engineered variants of Cas9 have been developed to improve specificity, but at the cost of the on-target ac
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3cc1a81570f9928f8cb73cc90029a715
https://doi.org/10.1093/nar/gkz1058
https://doi.org/10.1093/nar/gkz1058
Publikováno v:
Journal of Visualized Experiments.
The development of clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (Cas9) into therapeutic modalities requires the avoidance of its potentially deleterious off-target effects. Several methods have been devised
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1824b0454e8af8119ebb3424b23a4041
https://doi.org/10.3791/59202
https://doi.org/10.3791/59202
Publikováno v:
Molecules and Cells
Programmable nucleases, which include zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and RNA-guided engineered nucleases (RGENs) repurposed from the type II clustered, regularly interspaced short palindromic r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0fd6b9b0a1237624f85495ab9360e802
https://doi.org/10.14348/molcells.2015.0103
https://doi.org/10.14348/molcells.2015.0103
Autor:
Minhee Jung, Eunji Shin, Young-Hoon Kim, Daesik Kim, Joonsun Lee, Kang-In Lee, Euihwan Jeong, Seokjoong Kim, Jungjoon K Lee, Jin-Soo Kim
The use of CRISPR-Cas9 as a therapeutic reagent is hampered by its off-target effects. Although rationally designed S. pyogenes Cas9 (SpCas9) variants that display higher specificities than the wild-type SpCas9 protein are available, these attenuated
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::76ca124aa3f66a466b2989de91c99e83
https://doi.org/10.1101/237040
https://doi.org/10.1101/237040