Zobrazeno 1 - 10
of 15
pro vyhledávání: '"June I. Pounder"'
Autor:
Leonid Heifets, Max Salfinger, Jenny Jo Graham, Cathy A. Petti, June I. Pounder, Douglas R. Storts, Karl V. Voelkerding, Jillian Dormandy, Akos Somoskovi, Clint M. Anderson
Publikováno v:
Diagnostic Microbiology and Infectious Disease. 67:101-105
Differentiation of Mycobacterium tuberculosis complex (MTC) species is important for patient management. We developed a genomic deletion assay based on multiplex polymerase chain reaction with melting temperature analysis that correctly identified 12
Autor:
June I. Pounder, Keith E. Simmon, Mary E. Brandt, Claudia A. Barton, Cathy A. Petti, Sheri Hohmann
Publikováno v:
Journal of Clinical Microbiology. 45:568-571
Fungal infections are increasing, particularly among immunocompromised hosts, and a rapid diagnosis is essential to initiate antifungal therapy. Often fungi cannot be identified by conventional methods and are classified as nonsporulating molds (NSM)
Publikováno v:
Journal of Clinical Microbiology. 44:2977-2982
The performance of repetitive-sequence-based PCR (rep-PCR) using the DiversiLab system for identification of Coccidioides species, Blastomyces dermatitidis , and Histoplasma capsulatum was assessed by comparing data obtained to colony morphology and
Publikováno v:
Diagnostic Microbiology and Infectious Disease. 54:217-222
The performance of a real-time polymerase chain reaction (PCR) assay using the Smart Cycler instrument and a minor groove binding MGB Eclipse probe (Epoch Biosciences, Bothell, WA) for identification of Mycobacterium tuberculosis complex in acid-fast
Publikováno v:
Journal of Clinical Microbiology. 43:2141-2147
The performance of repetitive-sequence-based PCR (rep-PCR) using the DiversiLab system for identification of dermatophytes commonly isolated in a clinical laboratory was assessed by comparing results to those of conventional tests (colony morphology,
Autor:
June I. Pounder, James A. Sweigard, Karen Tenney, Barry J. Bowman, Chadonna McClain, Emma Jean Bowman, Ian Hunt
Publikováno v:
Fungal Genetics and Biology. 31:205-217
We have identified a gene, named hex-1, that encodes the major protein in the hexagonal crystals, or Woronin bodies, of Neurospora crassa. Analysis of a strain with a null mutation in the hex-1 gene showed that the septal pores in this organism were
Autor:
Frank Walsh, Cathy A. Petti, Keith E. Simmon, June I. Pounder, John N. Greene, Samuel Cohen, Clint M. Anderson
Publikováno v:
Journal of Clinical Microbiology. 45:1978-1980
Mycobacterium massiliense is a rapidly growing mycobacterium that is indistinguishable from Mycobacterium chelonae / M. abscessus by partial 16S rRNA gene sequencing. We sequenced rpoB , sodA , and hsp65 genes from isolates previously identified as b
Publikováno v:
Clinical Chemistry. 52:1443-1445
Multiplex, closed-tube detection of PCR products usually requires expensive probes of different colors. Alternatively, many PCR products can be distinguished by the amplicon melting temperature ( T m) in a single color with saturating DNA dyes. We de
Autor:
Jay J. Meyer, Joann L. Cloud, Gail L. Woods, June I. Pounder, Karen C. Carroll, Amy Sweeney, Kenneth C. Jost
Publikováno v:
International journal of systematic and evolutionary microbiology. 56(Pt 6)
SeveralMycobacterium-like organisms related to theMycobacterium terraecomplex have been isolated from clinical samples. In the clinical microbiology laboratory, partial 16S rRNA gene sequencing (approximately the first 500 bp) rather than full 16S rR
Publikováno v:
Journal of clinical microbiology. 43(12)
During the implementation of a PCR-based assay to differentiate between Mycobacterium abscessus and M. chelonae using the 16S to 23S internal transcribed spacer region ([1][1]), a sequence variant of M. abscessus was discovered. The sequence variant