Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Jun-ichi Ryu"'
Publikováno v:
Gene. 167:59-62
A unique DNA restriction-modification (R-M) system has been identified in the GM236 strain of Klebsiella pneumoniae using the newly isolated phage, SBS. The system was designated KpnBI. The gene (hsdRKpnBI) complementing the restriction activity of t
Publikováno v:
Moleculargeneral genetics : MGG. 241(5-6)
The type I restriction and modification genes of Escherichia coli can be transferred to other non-modified strains by conjugation without killing the recipient, implying that the restriction function must be regulated. In this study, two isogenic F
Publikováno v:
Gene. 99(1)
Genomic (chromosomal) hsd -Mu( lac ) operon fusions have been constructed in two strains of Escherichia coli K-12 for the three hsd genes, hsdR K , hsdM K and hasS K , using MudX and λp lac Mu53. Expression of hsdK mutants ranged from 16 to 74 units
Autor:
Ainu Prakash-Cheng, Jun-ichi Ryu
Publikováno v:
Journal of Bacteriology. 175:4905-4906
Following conjugal transfer of the hsdK genes (hsdRK, hsdMK, and hsdSK) of Escherichia coli K-12, restriction activity was first detected only after approximately 15 generations, whereas modification activity was observed immediately. This sequential
Publikováno v:
Journal of general microbiology. 135(9)
SUMMARY: Stable restriction-deficient, modification-proficient galE (JR501) and F‘galE + (JR502) strains of Salmonella typhimurium were constructed and the effects of restriction on transformation by plasmid pBR322 were tested. Several factors whic
Publikováno v:
Journal of bacteriology. 170(7)
Mu dX(lac) insertion mutants of Escherichia coli CSH50 in which the expression of the lacZ gene was sensitive to the presence of exogenous 1-thioglycerol or dithiothreitol were isolated. Both stimulatory and inhibitory mutants were found. The existen
Publikováno v:
Journal of bacteriology. 170(12)
Of eight Salmonella, serB-linked hsd genes for the restriction and modification of DNA transferred to Escherichia coli/Salmonella hybrids, only two--those with SM and ST (S. muenchen and S. thompson, respectively) specificities--may have weakly compl