Zobrazeno 1 - 10
of 77
pro vyhledávání: '"Jun-ichi Horiuchi"'
Autor:
Haingomaholy Michelle Rakotondravao, Ryosuke Takahashi, Takatomo Takai, Yumiko Sakoda, Jun-Ichi Horiuchi, Yoichi Kumada
Publikováno v:
JOURNAL OF CHEMICAL ENGINEERING OF JAPAN. 55:267-274
Autor:
Koichi Takahashi, Reina Tanibata, Yohei Miyamura, Jun-ichi Horiuchi, Yoichi Kumada, Fumio Gondaira, Shinya Ogasawara
Publikováno v:
Journal of Bioscience and Bioengineering. 131:84-89
In this study, immobilization of single-chain Fv (scFv) antibodies on the surfaces of polystyrene (PS) latex beads via material-binding peptides was investigated for sensitive immuno-turbidimetric assay of C-reactive protein (CRP). Anti-CRP scFvs fus
Autor:
Yoichi Kumada, Haingomaholy Michelle Rakotondravao, Yuya Hasegawa, Yuki Iwashita, Hiromichi Okura, Seiichi Uchimura, Jun-ichi Horiuchi
Publikováno v:
Journal of bioscience and bioengineering. 134(3)
We developed affinity chromatographic resins that immobilized rabbit single-chain Fv antibodies (scFvs). By biopanning using antigen-coupled multilamellar vesicles (Ag-MLVs), 152 types of original scFv clones that specifically bind to human IgG were
A metabolically engineered Lactobacillus plantarum mutant, which could produce D-lactic acid from both glucose and xylose, was applied for the production of optically pure D-lactic acid from corncobs by simultaneous saccharification and fermentation
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::18314b3c27474b50db0898e739d2bbff
https://doi.org/10.22541/au.161774066.61150349/v1
https://doi.org/10.22541/au.161774066.61150349/v1
Autor:
Yoichi Kumada, Ryosuke Takahashi, Ryota Wada, Emi Ichihashi, Jun-ichi Horiuchi, Haingomaholy Michelle Rakotondravao, Takatomo Takai, Norio Ishizuka, Sakakibara Keita
Publikováno v:
Journal of chromatography. A. 1656
This study investigated the adsorption capacity and mass transfer properties of a novel macroporous epoxy-polymer-based anion-exchanger, MPR Q, for the efficient separation of therapeutic proteins. MPR Q resin was prepared by phase separation based o
Publikováno v:
Journal of bioscience and bioengineering. 132(1)
Dissolved oxygen (DO)-stat fed-batch culture, which allows a high cell density culture of microorganisms under constant DO conditions, was applied to anti-CRP single-chain variable fragment (scFv) production using recombinant Escherichia coli. The DO
Publikováno v:
Journal of bioscience and bioengineering. 131(3)
We demonstrated an efficient screening method for rabbit scFv antibodies using antigen-coupled multi-lamellar vesicles (Ag-MLVs) as solid supports. Model phages displaying mouse anti-human IgG scFv at a probability of 10−4–10−5% were successful
Publikováno v:
Biochemical Engineering Journal. 176:108184
The stabilized production of anti-CRP single-chain Fv (scFv) antibody using recombinant E. coli by prolonged dissolved oxygen (DO)-stat fed-batch culture was demonstrated, focusing on the supply of nitrogen sources during fed-batch operation. The ser
Publikováno v:
Biotechnology Progress. 31:1563-1570
A PMMA-binding peptide (PMMA-tag) was genetically fused with the C-terminal region of an anti-human chorionic gonadotropin (hCG) single-domain antibody (VHH). It was over-expressed in an insoluble fraction of E. coli cells, and recovered in the prese
Publikováno v:
Journal of Bioscience and Bioengineering. 119:317-322
Sophorolipids (SLs) are amphiphilic compounds produced from a variety of saccharides and vegetable oils by the yeast Starmerella bombicola and related strains, and they have commercial uses as detergents. In the present study, SL production was inves