Zobrazeno 1 - 10
of 107
pro vyhledávání: '"Jun-ichi Horiuchi"'
Autor:
Haingomaholy Michelle Rakotondravao, Ryosuke Takahashi, Takatomo Takai, Yumiko Sakoda, Jun-Ichi Horiuchi, Yoichi Kumada
Publikováno v:
JOURNAL OF CHEMICAL ENGINEERING OF JAPAN. 55:267-274
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4d250b9dd96368c2dc917b9b23eff9c2
https://doi.org/10.1252/jcej.22we035
https://doi.org/10.1252/jcej.22we035
Autor:
Koichi Takahashi, Reina Tanibata, Yohei Miyamura, Jun-ichi Horiuchi, Yoichi Kumada, Fumio Gondaira, Shinya Ogasawara
Publikováno v:
Journal of Bioscience and Bioengineering. 131:84-89
In this study, immobilization of single-chain Fv (scFv) antibodies on the surfaces of polystyrene (PS) latex beads via material-binding peptides was investigated for sensitive immuno-turbidimetric assay of C-reactive protein (CRP). Anti-CRP scFvs fus
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dddbbeb53f913b5a98d919e07bef7e8c
https://doi.org/10.1016/j.jbiosc.2020.08.014
https://doi.org/10.1016/j.jbiosc.2020.08.014
Autor:
Yoichi Kumada, Haingomaholy Michelle Rakotondravao, Yuya Hasegawa, Yuki Iwashita, Hiromichi Okura, Seiichi Uchimura, Jun-ichi Horiuchi
Publikováno v:
Journal of bioscience and bioengineering. 134(3)
We developed affinity chromatographic resins that immobilized rabbit single-chain Fv antibodies (scFvs). By biopanning using antigen-coupled multilamellar vesicles (Ag-MLVs), 152 types of original scFv clones that specifically bind to human IgG were
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba960c2633828df35b8014926bce9322
https://pubmed.ncbi.nlm.nih.gov/35798650
https://pubmed.ncbi.nlm.nih.gov/35798650
A metabolically engineered Lactobacillus plantarum mutant, which could produce D-lactic acid from both glucose and xylose, was applied for the production of optically pure D-lactic acid from corncobs by simultaneous saccharification and fermentation
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::18314b3c27474b50db0898e739d2bbff
https://doi.org/10.22541/au.161774066.61150349/v1
https://doi.org/10.22541/au.161774066.61150349/v1
Autor:
Yoichi Kumada, Ryosuke Takahashi, Ryota Wada, Emi Ichihashi, Jun-ichi Horiuchi, Haingomaholy Michelle Rakotondravao, Takatomo Takai, Norio Ishizuka, Sakakibara Keita
Publikováno v:
Journal of chromatography. A. 1656
This study investigated the adsorption capacity and mass transfer properties of a novel macroporous epoxy-polymer-based anion-exchanger, MPR Q, for the efficient separation of therapeutic proteins. MPR Q resin was prepared by phase separation based o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c5b3751b9af60d28b0c89836192b7a0a
https://pubmed.ncbi.nlm.nih.gov/34520891
https://pubmed.ncbi.nlm.nih.gov/34520891
Publikováno v:
Journal of bioscience and bioengineering. 132(1)
Dissolved oxygen (DO)-stat fed-batch culture, which allows a high cell density culture of microorganisms under constant DO conditions, was applied to anti-CRP single-chain variable fragment (scFv) production using recombinant Escherichia coli. The DO
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ca8454bdb1b20827cc71556e06d0c842
https://pubmed.ncbi.nlm.nih.gov/33896701
https://pubmed.ncbi.nlm.nih.gov/33896701
Completely revised, updated, and enlarged, this second edition now contains a subchapter on biorecognition assays, plus a chapter on bioprocess control added by the new co-author Jun-ichi Horiuchi, who is one of the leading experts in the field. The
Publikováno v:
Journal of bioscience and bioengineering. 131(3)
We demonstrated an efficient screening method for rabbit scFv antibodies using antigen-coupled multi-lamellar vesicles (Ag-MLVs) as solid supports. Model phages displaying mouse anti-human IgG scFv at a probability of 10−4–10−5% were successful
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5a232459574b09124a01f2c7654f2c47
https://pubmed.ncbi.nlm.nih.gov/33221176
https://pubmed.ncbi.nlm.nih.gov/33221176
Publikováno v:
Biochemical Engineering Journal. 176:108184
The stabilized production of anti-CRP single-chain Fv (scFv) antibody using recombinant E. coli by prolonged dissolved oxygen (DO)-stat fed-batch culture was demonstrated, focusing on the supply of nitrogen sources during fed-batch operation. The ser
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b05d10b1c0d614353b3e05c536e77733
https://doi.org/10.1016/j.bej.2021.108184
https://doi.org/10.1016/j.bej.2021.108184
Publikováno v:
Biotechnology Progress. 31:1563-1570
A PMMA-binding peptide (PMMA-tag) was genetically fused with the C-terminal region of an anti-human chorionic gonadotropin (hCG) single-domain antibody (VHH). It was over-expressed in an insoluble fraction of E. coli cells, and recovered in the prese
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0fc6c8f9213c3830519096e690add8f9
https://doi.org/10.1002/btpr.2169
https://doi.org/10.1002/btpr.2169