Combination of CENP-B Box Positive and Negative Synthetic Alpha Satellite Repeats Improves De Novo Human Artificial Chromosome Formation

Autor: Koei Okazaki, Megumi Nakano, Jun-ichirou Ohzeki, Koichiro Otake, Kazuto Kugou, Vladimir Larionov, William C. Earnshaw, Hiroshi Masumoto

Publikováno v: Cells, Vol 11, Iss 9, p 1378 (2022)

Human artificial chromosomes (HACs) can be formed de novo by introducing large (>30 kb) centromeric sequences consisting of highly repeated 171-bp alpha satellite (alphoid) DNA into HT1080 cells. However, only a subset of transformed cells successful

Externí odkaz: https://doaj.org/article/7ed9bd2928dc495dbaf0357bfe984312

Zinc-finger protein 518 plays a crucial role in pericentromeric heterochromatin formation by linking satellite DNA to heterochromatin

Autor: Shinya Ohta, Jun-Ichirou Ohzeki, Nobuko Sato, Ken-Ichi Noma, Hiroshi Masumoto

SummaryAneuploidy is caused by chromosomal missegregation and is frequently observed in cancers and hematological diseases. Pericentromeric heterochromatin formation is essential for proper chromosome segregation; however, it is unclear how heterochr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::85113174a7278bed21c0dfe1feb1e78f
https://doi.org/10.1101/2022.09.15.508097

De novo formation and epigenetic maintenance of centromere chromatin

Autor: Hiroshi Masumoto, William C. Earnshaw, Vladimir Larionov, Jun-ichirou Ohzeki

Publikováno v: Curr Opin Cell Biol
Ohzeki, J, Larionov, V, Earnshaw, W C & Masumoto, H 2019, ' De novo formation and epigenetic maintenance of centromere chromatin ', Current opinion in cell biology, vol. 58, pp. 15-25 . https://doi.org/10.1016/j.ceb.2018.12.004

Accurate chromosome segregation is essential for cell proliferation. The centromere is a specialized chromosomal locus, on which the kinetochore structure is formed. The centromere/kinetochore is required for the equal separation of sister chromatids

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9c5a4707dcf93dfd1afd242097bad445
https://pubmed.ncbi.nlm.nih.gov/30654232

Stable complex formation of CENP-B with the CENP-A nucleosome

Autor: Yasuhiro Arimura, Vladimir Larionov, Yuta Miya, Hiroshi Masumoto, Hiroaki Tachiwana, Akihisa Osakabe, Tatsuya Shiga, Hitoshi Kurumizaka, Risa Fujita, Jun Ichirou Ohzeki, Koichiro Otake, Naoki Horikoshi

Publikováno v: Nucleic Acids Research

CENP-A and CENP-B are major components of centromeric chromatin. CENP-A is the histone H3 variant, which forms the centromere-specific nucleosome. CENP-B specifically binds to the CENP-B box DNA sequence on the centromere-specific repetitive DNA. In

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fe46b78346e7e0e1b7bbea809b0f9f07
http://europepmc.org/articles/PMC4446444

Breaking the HAC Barrier: Histone H3K9 acetyl/methyl balance regulates CENP-A assembly

Autor: Hiroshi Masumoto, Natalay Kouprina, Vladimir Larionov, Vladimir N. Noskov, Jan H. Bergmann, William C. Earnshaw, Megumi Nakano, Hiroshi Kimura, Jun-ichirou Ohzeki

Publikováno v: The EMBO Journal. 31:2391-2402

The kinetochore is responsible for accurate chromosome segregation. However, the mechanism by which kinetochores assemble and are maintained remains unclear. Here we report that de novo CENP-A assembly and kinetochore formation on human centromeric a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::e9ba9969de6651658eaa85516e8ebd30
https://doi.org/10.1038/emboj.2012.82