Zobrazeno 1 - 10
of 48
pro vyhledávání: '"Jun He Zhang"'
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 10 (2022)
Recombinant antibodies are rapidly developing therapeutic agents; approximately 40 novel antibody molecules enter clinical trials each year, most of which are produced from Chinese hamster ovary (CHO) cells. However, one of the major bottlenecks rest
Externí odkaz:
https://doaj.org/article/b5f910dbfa554cfa908c625be4d0a51b
Esophageal cancer (EC) is the tenth most common cancer worldwide and has high morbidity and mortality. Its main subtypes include esophageal squamous cell carcinoma and esophageal adenocarcinoma, which are usually diagnosed in their late stages. The b
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4a2d7215a1525aea5d0fb34cfa2e9b64
https://doi.org/10.22541/au.167689984.41968125/v1
https://doi.org/10.22541/au.167689984.41968125/v1
Publikováno v:
Energy & Environment, 2013 Jan 01. 24(6), 1043-1049.
Externí odkaz:
https://www.jstor.org/stable/43735218
Publikováno v:
Molecular Biology Reports. 47:5185-5190
The aim of this study was to construct an expression vector mediated by the dual promoter that can simultaneously drive the recombinant protein production in eukaryotic and prokaryotic cells. The prokaryotic T7 promoter and ribosome binding site (RBS
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::53266ea90348eb856926252f2529cafa
https://doi.org/10.1007/s11033-020-05593-2
https://doi.org/10.1007/s11033-020-05593-2
Publikováno v:
Biotechnology Letters. 42:187-196
Previously, we have found that the matrix attachment region (MAR) may confer a ‘distance effect’ on transgene expression. This work aims to systematically explore the increased transgene expression in transfected Chinese hamster ovary (CHO) cells
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::368fc4cc9af6049f9ec1b43ed5378365
https://doi.org/10.1007/s10529-019-02775-2
https://doi.org/10.1007/s10529-019-02775-2
Publikováno v:
PLoS ONE, Vol 6, Iss 8, p e22394 (2011)
A fixation-free and fast protein-staining method for sodium dodecyl sulfate-polyacrylamide gel electrophoresis using Coomassie blue is described. The protocol comprises staining and quick washing steps, which can be completed in 0.5 h. It has a sensi
Externí odkaz:
https://doaj.org/article/e7560fc711814b81921fcb9a8ebe1837
Publikováno v:
Journal of Nucleic Acids, Vol 2010 (2010)
DNA molecular weight standard control, also called DNA marker (ladder), has been widely used in the experiments of molecular biology. In the paper, we report a method by which DNA marker was prepared based on multiple PCR technique. 100–1000 bp DNA
Externí odkaz:
https://doaj.org/article/f9052a2e57f44797890713183bd21d6b
Autor:
Zhong-Jie Xu, Jun-He Zhang, Xiao-Yin Wang, Tian-Yun Wang, Zheng-Wei Tian, Xu Danhua, Xi Zhang, Qin Li
Publikováno v:
Journal of Cellular and Molecular Medicine
In our previous study, we demonstrated that episomal vectors based on the characteristic sequence of matrix attachment regions (MARs) and containing the cytomegalovirus (CMV) promoter allow transgenes to be maintained episomally in Chinese hamster ov
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e4abfd186bd4228ec3d49a5a8b25bc19
http://europepmc.org/articles/PMC5661254
http://europepmc.org/articles/PMC5661254
Publikováno v:
Bioengineered, Vol 10, Iss 1, Pp 548-560 (2019)
Bioengineered
Bioengineered
The episomal vector cannot integrate into the host cell chromosome, which has no potential risk in gene therapy. However, the low level of transgene expression driven by episomal vectors needs to be solved. In this study, we investigated the effects
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::623806317c0a9fbe54a6bcf787877885
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1853
Most traditional post-electrophoretic processes need several hours to several days to finish the whole staining process and traditional staining solutions all contain methanol, acetic acid, or phosphoric acid, which not only produce the unpleasant sm
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::62c092f8ba421862dbd0c4518dbd3287
https://pubmed.ncbi.nlm.nih.gov/30097927
https://pubmed.ncbi.nlm.nih.gov/30097927