Zobrazeno 1 - 6
of 6
pro vyhledávání: '"July Dorion-Thibaudeau"'
Autor:
Anne-Marie Gélinas, Howard Kim, July Dorion-Thibaudeau, Alice Bernier, Aziza P. Manceur, Sven Ansorge, Vanja Misic, Nadejda Andreev, Rénald Gilbert, Stéphane Lanthier, Sophie Broussau, Sonia Tremblay
Publikováno v:
Human Gene Therapy Methods
Lentiviral vectors (LV) represent a key tool for gene and cell therapy applications. The production of these vectors in sufficient quantities for clinical applications remains a hurdle, prompting the field towards developing suspension processes that
Publikováno v:
Molecular Immunology. 82:46-49
A surface plasmon resonance (SPR)-based assay has been developed in order to quantify the monoclonal antibody (Mab) Trastuzumab within the supernatant of a mammalian cell culture using the ectodomain of FcγRI (CD64) and confirm Mab bioactivity, i.e.
Autor:
Gregory De Crescenzo, Yves Durocher, Trushar R. Patel, Matthew McDougall, Jörg Stetefeld, Markus Meier, Michael Butler, Maureen Spearman, July Dorion-Thibaudeau, Natalie Krahn
Publikováno v:
European Journal of Pharmaceutical Sciences. 96:428-439
Glycoengineering of mAbs has become common practice in attempts to generate the ideal mAb candidate for a wide range of therapeutic applications. The effects of these glycan modifications on the binding affinity of IgG mAbs for FcγRIIIa and their cy
Autor:
Céline Raymond, Gregory De Crescenzo, July Dorion-Thibaudeau, Gilles St-Laurent, Yves Durocher
Publikováno v:
Journal of Molecular Recognition. 29:60-69
We here report the production of four biotinylated Fcγ receptor (FcγR) ectodomains and their subsequent stable capture on streptavidin-biosensor surfaces. For receptor biotinylation, we first describe an in-cell protocol based on the co-transfectio
Autor:
Gregory De Crescenzo, July Dorion-Thibaudeau, Erika Lattová, Céline Raymond, Hélène Perreault, Yves Durocher
Publikováno v:
Journal of Immunological Methods. 408:24-34
We here report the production and purification of the extracellular domains of two Fcγ receptors, namely CD16a and CD64, by transient transfection in mammalian cells. The use of these two receptor ectodomains for the development of quantitative assa
Autor:
July, Dorion-Thibaudeau, Gilles, St-Laurent, Céline, Raymond, Gregory, De Crescenzo, Yves, Durocher
Publikováno v:
Journal of molecular recognition : JMR. 29(2)
We here report the production of four biotinylated Fcγ receptor (FcγR) ectodomains and their subsequent stable capture on streptavidin-biosensor surfaces. For receptor biotinylation, we first describe an in-cell protocol based on the co-transfectio