Sense codon reassignment enables viral resistance and encoded polymer synthesis

Autor: Sarah L. Maslen, Salvador Buse, Martin Spinck, Louise F. H. Funke, Daniel de la Torre, George P. C. Salmond, Wesley E. Robertson, Thomas S. Elliott, Franz L. Böge, Daniele Cervettini, Julius Fredens, Jason W. Chin, Yonka Christova, Kim C. Liu

Publikováno v: Science. 372:1057-1062

Designing bacterial superpowers Biological systems read all 64 triplet codons in DNA to encode the synthesis of proteins composed of 20 canonical amino acids. Robertson et al. created cells that do not read several codons and showed that this confers

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f5f1add2abd9e8d7046f897f7ff53b62
https://doi.org/10.1126/science.abg3029

Creating custom synthetic genomes in Escherichia coli with REXER and GENESIS

Autor: Wesley E, Robertson, Louise F H, Funke, Daniel, de la Torre, Julius, Fredens, Kaihang, Wang, Jason W, Chin

Publikováno v: Nature protocols. 16(5)

We previously developed REXER (Replicon EXcision Enhanced Recombination); this method enables the replacement of100 kb of the Escherichia coli genome with synthetic DNA in a single step and allows the rapid identification of non-viable or otherwise p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::7faeed7982cea245d2ef31cb2136ad77
https://pubmed.ncbi.nlm.nih.gov/33903757

Axon-Dependent Patterning and Maintenance of Somatosensory Dendritic Arbors

Autor: Benjamin Raja, Nelson J. Ramirez-Suarez, David H. Hall, Helen M. Belalcazar, Hannes E. Bülow, Christopher J. Salazar, Burcu Beyaz, Ken C. Q. Nguyen, Julius Fredens, Kevin Celestrin, Nils J. Færgeman

Publikováno v: Ramirez-Suarez, N J, Belalcazar, H M, Salazar, C J, Beyaz, B, Raja, B, Nguyen, K C Q, Celestrin, K, Fredens, J, Færgeman, N J, Hall, D H & Bülow, H E 2019, ' Axon-Dependent Patterning and Maintenance of Somatosensory Dendritic Arbors ', Developmental Cell, vol. 48, no. 2, pp. 229-244.e4 . https://doi.org/10.1016/j.devcel.2018.12.015

The mechanisms that pattern and maintain dendritic arbors are key to understanding the principles that govern nervous system assembly. The activity of presynaptic axons has long been known to shape dendrites, but activity-independent functions of axo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::67eb04c10c4e56e655a92a50fbe018f4
https://doi.org/10.1016/j.devcel.2018.12.015

Identification of novel protein functions and signaling mechanisms by genetics and quantitative phosphoproteomics in Caenorhabditis elegans

Autor: Julius, Fredens, Kasper, Engholm-Keller, Jakob, Møller-Jensen, Martin Røssel, Larsen, Nils J, Færgeman

Publikováno v: Methods in molecular biology (Clifton, N.J.). 1188

Stable isotope labeling by amino acids combined with mass spectrometry is a widely used methodology for measuring relative changes in protein and phosphorylation levels at a global level. We have applied this method to the model organism Caenorhabdit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::cab2a648a0ce454d91add739ff8f89bb
https://pubmed.ncbi.nlm.nih.gov/25059608

A method for measuring fatty acid oxidation in C. elegans

Autor: Ida Coordt Elle, Steven Vestergaard Rødkær, Julius Fredens, Nils J. Færgeman

Publikováno v: Worm
Elle, I C, Rødkær, S V, Fredens, J & Færgeman, N J 2012, ' A method for measuring fatty acid oxidation in C. elegans ', Worm, vol. 1, no. 1, pp. 26-30 . https://doi.org/10.4161/worm.19564

The nematode C. elegans has during the past decade proven to be a valuable model organism to identify and examine molecular mechanisms regulating lipid storage and metabolism. While the primary approach has been to identify genes and pathways conferr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::14525bc78aa46ed2131cd8bb47da30d3
https://pubmed.ncbi.nlm.nih.gov/24058820