Design and realization of a microfluidic device devoted to the application of ultra-short pulses of electrical field to living cells

Autor: Julien Villemejane, Lluis M. Mir, Bruno Le Pioufle, Olivier Français, Claire Dalmay, Vanessa Joubert

Publikováno v: Sensors and Actuators B: Chemical
Sensors and Actuators B: Chemical, Elsevier, 2011, 160 (1), pp.1573-1580. ⟨10.1016/j.snb.2011.09.009⟩

0925-4005; International audience; In this paper, we present a novel microfluidic system dedicated to the application of ultra short pulses (i.e. nanopulses) on cells and the visualization of their effects. Cell plasma membranes can be rendered perme

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f7ed06829ab944adaf656f0644fa8fda
https://doi.org/10.1016/j.snb.2011.09.009

Physical methods of nucleic acid transfer: general concepts and applications

Autor: Julien Villemejane, Lluis M. Mir

Publikováno v: British Journal of Pharmacology. 157:207-219

Physical methods of gene (and/or drug) transfer need to combine two effects to deliver the therapeutic material into cells. The physical methods must induce reversible alterations in the plasma membrane to allow the direct passage of the molecules of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::0331279373ffc48a86132f3dc8b47b2c
https://doi.org/10.1111/j.1476-5381.2009.00032.x

Electrical modeling of the influence of medium conductivity on electroporation

Autor: Lluis M. Mir, Julien Villemejane, Antoni Ivorra

Publikováno v: Recercat. Dipósit de la Recerca de Catalunya
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Electroporation is the phenomenon in which cell membrane permeability is increased by exposing the cell to short high electric field pulses. Experimental data show that the amount of permeabilization depends on the conductivity of the extracellular m

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4be46545989b3a1db3bb07599855ee2d
https://pubmed.ncbi.nlm.nih.gov/20585676

A technique to design complex 3D lab on a chip involving multilayered fluidics, embedded thick electrodes and hard packaging. Application to dielectrophoresis and electroporation of cells

Autor: Lluis M. Mir, Guillaume Mottet, B. Le Pioufle, Julien Villemejane

Publikováno v: Journal of Micromechanics and Microengineering
Journal of Micromechanics and Microengineering, IOP Publishing, 2010, 20, pp.047001. ⟨10.1088/0960-1317/20/4/047001⟩

International audience; Nowadays, lab on chips (LOCs) require the development of new technologies in order to integrate complex fluidics, sensors, actuators, etc. Such integration requires overcoming both technological bottlenecks and an increase in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b07bcaa0125a29cfaeeb53cceac3147a
https://hal.archives-ouvertes.fr/hal-00592400