VCF1 is a p97/VCP cofactor promoting recognition of ubiquitylated p97-UFD1-NPL4 substrates

Autor: Ann Schirin Mirsanaye, Saskia Hoffmann, Melanie Weisser, Andreas Mund, Blanca Lopez Mendez, Dimitris Typas, Johannes van den Boom, Bente Benedict, Ivo A. Hendriks, Michael Lund Nielsen, Hemmo Meyer, Julien P. Duxin, Guillermo Montoya, Niels Mailand

Publikováno v: Nature Communications, Vol 15, Iss 1, Pp 1-16 (2024)

Abstract The hexameric AAA+ ATPase p97/VCP functions as an essential mediator of ubiquitin-dependent cellular processes, extracting ubiquitylated proteins from macromolecular complexes or membranes by catalyzing their unfolding. p97 is directed to ub

Externí odkaz: https://doaj.org/article/94acc0facb2c40838b11f84474d25e74

A CRISPR-Cas9 screen identifies EXO1 as a formaldehyde resistance gene

Autor: Yuandi Gao, Laure Guitton-Sert, Julien Dessapt, Yan Coulombe, Amélie Rodrigue, Larissa Milano, Andréanne Blondeau, Nicolai Balle Larsen, Julien P. Duxin, Samer Hussein, Amélie Fradet-Turcotte, Jean-Yves Masson

Publikováno v: Nature Communications, Vol 14, Iss 1, Pp 1-20 (2023)

Formaldehyde can trigger formation of interstrand crosslinks (ICLs) or DNA-protein crosslinks (DPCs) leading to genome instability. Here the authors show that EXO1 limits replication stress and DNA damage to counteract formaldehyde-induced genome ins

Externí odkaz: https://doaj.org/article/70dd8826225249e28b7b7b6e5a01c276

Targeting DNA-Protein Crosslinks via Post-Translational Modifications

Autor: Xueyuan Leng, Julien P. Duxin

Publikováno v: Frontiers in Molecular Biosciences, Vol 9 (2022)

Covalent binding of proteins to DNA forms DNA-protein crosslinks (DPCs), which represent cytotoxic DNA lesions that interfere with essential processes such as DNA replication and transcription. Cells possess different enzymatic activities to countera

Externí odkaz: https://doaj.org/article/6525f20037d14c588267e56e6bf8d131

Crosstalk between repair pathways elicits double-strand breaks in alkylated DNA and implications for the action of temozolomide

Autor: Robert P Fuchs, Asako Isogawa, Joao A Paulo, Kazumitsu Onizuka, Tatsuro Takahashi, Ravindra Amunugama, Julien P Duxin, Shingo Fujii

Publikováno v: eLife, Vol 10 (2021)

Temozolomide (TMZ), a DNA methylating agent, is the primary chemotherapeutic drug used in glioblastoma treatment. TMZ induces mostly N-alkylation adducts (N7-methylguanine and N3-methyladenine) and some O6-methylguanine (O6mG) adducts. Current models

Externí odkaz: https://doaj.org/article/0d670b1a8f304282bd6a3a696de67cd0

SCAI promotes error-free repair of DNA interstrand crosslinks via the Fanconi anemia pathway

Autor: Lisa Schubert, Ivo A Hendriks, Emil P T Hertz, Wei Wu, Selene Sellés‐Baiget, Saskia Hoffmann, Keerthana Stine Viswalingam, Irene Gallina, Satyakrishna Pentakota, Bente Benedict, Joachim Johansen, Katja Apelt, Martijn S Luijsterburg, Simon Rasmussen, Michael Lisby, Ying Liu, Michael L Nielsen, Niels Mailand, Julien P Duxin

Publikováno v: EMBO Reports, 23(4). WILEY
Schubert, L, Hendriks, I A, Hertz, E P T, Wu, W, Sellés-Baiget, S, Hoffmann, S, Viswalingam, K S, Gallina, I, Pentakota, S, Benedict, B, Johansen, J, Apelt, K, Luijsterburg, M S, Rasmussen, S, Lisby, M, Liu, Y, Nielsen, M L, Mailand, N & Duxin, J P 2022, ' SCAI promotes error-free repair of DNA interstrand crosslinks via the Fanconi anemia pathway ', EMBO Reports, vol. 23, no. 4, e53639 . https://doi.org/10.15252/embr.202153639

DNA interstrand crosslinks (ICLs) are cytotoxic lesions that threaten genome integrity. The Fanconi anemia (FA) pathway orchestrates ICL repair during DNA replication, with ubiquitylated FANCI-FANCD2 (ID2) marking the activation step that triggers in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b635fe1b7eb375726bd27f3881f73f4
https://doi.org/10.15252/embr.202153639