High-yield overproduction and purification of human aquaporins from Pichia pastoris

Autor: Tamim Al-Jubair, Jonas Hyld Steffen, Julie Winkel Missel, Philip Kitchen, Mootaz M. Salman, Roslyn M. Bill, Pontus Gourdon, Susanna Törnroth-Horsefield

Publikováno v: STAR Protocols, Vol 3, Iss 2, Pp 101298- (2022)

Summary: Aquaporins (AQPs) are membrane-bound water channels that play crucial roles in maintaining the water homeostasis of the human body. Here, we present a protocol for high-yield recombinant expression of human AQPs in the methylotropic yeast Pi

Externí odkaz: https://doaj.org/article/5ab3c0de13584bbd895ba84cf305b169

Cyclohexyl-α maltoside as a highly efficient tool for membrane protein studies

Autor: Julie Winkel Missel, Nina Salustros, Eva Ramos Becares, Jonas Hyld Steffen, Amalie Gerdt Laursen, Angelica Struve Garcia, Maria M. Garcia-Alai, Čeněk Kolar, Pontus Gourdon, Kamil Gotfryd

Publikováno v: Current Research in Structural Biology, Vol 3, Iss , Pp 85-94 (2021)

Membrane proteins (MPs) constitute a large fraction of the proteome, but exhibit physicochemical characteristics that impose challenges for successful sample production crucial for subsequent biophysical studies. In particular, MPs have to be extract

Externí odkaz: https://doaj.org/article/52c903b70c7b4014836baa03b5bbc555

Human adipose glycerol flux is regulated by a pH gate in AQP10

Autor: Kamil Gotfryd, Andreia Filipa Mósca, Julie Winkel Missel, Sigurd Friis Truelsen, Kaituo Wang, Mariana Spulber, Simon Krabbe, Claus Hélix-Nielsen, Umberto Laforenza, Graça Soveral, Per Amstrup Pedersen, Pontus Gourdon

Publikováno v: Nature Communications, Vol 9, Iss 1, Pp 1-11 (2018)

Uptake and release of glycerol from the small intestine and adipocytes is facilitated by a subclass of aquaporins (AQP), but how glycerol flow is regulated remains poorly understood. Here authors solve the crystal structure of AQP10 and show how lipo

Externí odkaz: https://doaj.org/article/f2f9e79e53f4454685084cc268e265dc

PcoB is a defense outer membrane protein that facilitates cellular uptake of copper

Autor: S. Abeyrathna, Magnus Andersson, P. Li, Kaituo Wang, K. Gorecki, Julie Winkel Missel, N. Nayeri, E. Ramos Becares, Gabriele Meloni, K. Lindkvist-Petersson, Dhani Ram Mahato, Pontus Gourdon

Publikováno v: Li, P, Nayeri, N, Górecki, K, Becares, E R, Wang, K, Mahato, D R, Andersson, M, Abeyrathna, S S, Lindkvist-Petersson, K, Meloni, G, Missel, J W & Gourdon, P 2022, ' PcoB is a defense outer membrane protein that facilitates cellular uptake of copper ', Protein Science, vol. 31, no. 7, e4364, pp. 1-16 . https://doi.org/10.1002/pro.4364

Copper (Cu) is one of the most abundant trace metals in all organisms, involved in a plethora of cellular processes. Yet elevated concentrations of the element are harmful, and interestingly prokaryotes are more sensitive for environmental Cu stress

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::15ee972cc3053fba0786392d57a2b32f
https://doi.org/10.1002/pro.4364

Cyclohexyl-alpha maltoside as a highly efficient tool for membrane protein studies

Autor: Angelica Struve Garcia, Eva Ramos Becares, Maria M. Garcia-Alai, Jonas Hyld Steffen, Amalie Gerdt Laursen, Kamil Gotfryd, Čeněk Kolar, Julie Winkel Missel, Pontus Gourdon, Nina Salustros

Publikováno v: Missel, J W, Salustros, N, Becares, E R, Steffen, J H, Laursen, A G, Garcia, A S, Garcia-Alai, M M, Kolar, C, Gourdon, P & Gotfryd, K 2021, ' Cyclohexyl-alpha maltoside as a highly efficient tool for membrane protein studies ', Current Research in Structural Biology, vol. 3, pp. 85-94 . https://doi.org/10.1016/j.crstbi.2021.03.002
Current Research in Structural Biology
Current Research in Structural Biology, Vol 3, Iss, Pp 85-94 (2021)

Membrane proteins (MPs) constitute a large fraction of the proteome, but exhibit physicochemical characteristics that impose challenges for successful sample production crucial for subsequent biophysical studies. In particular, MPs have to be extract

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::941963255f68fd52a1dfe455d8f5d86e
https://curis.ku.dk/ws/files/291023487/Cyclohexyl_maltoside_as_a_highly_efficient_tool_for_membrane.pdf