Zobrazeno 1 - 10
of 19
pro vyhledávání: '"Julie Donaghey"'
Autor:
Christina Galonska, Jocelyn Charlton, Alexandra L. Mattei, Julie Donaghey, Kendell Clement, Hongcang Gu, Arman W. Mohammad, Elena K. Stamenova, Davide Cacchiarelli, Sven Klages, Bernd Timmermann, Tobias Cantz, Hans R. Schöler, Andreas Gnirke, Michael J. Ziller, Alexander Meissner
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-9 (2018)
Catalytically inactive Cas9 fused to a methyltransferase has emerged as a promising epigenome modifying tool. Here the authors generate a methylation depleted but maintenance competent mouse ES cell line and find ubiquitous off-target activity.
Externí odkaz:
https://doaj.org/article/facfcb998fb94a91ab5dd5199cd3a1b4
Autor:
Douglas A. Melton, Alexander Meissner, Niloofar Rasouli, Juerg R. Straubhaar, Jennifer H. R. Kenty, Julie Donaghey, Juan R. Alvarez-Dominguez, Aharon Helman, Jocelyn Charlton
Publikováno v:
StemJournal. 2:7-13
Stem cell-derived tissues that recap endogenous physiology are key for regenerative medicine. Yet, most methods yield products that function like fetal, not adult tissues. Organoids are typically grown in constant environments, while our tissues matu
Autor:
Julie Donaghey, Cecilia Kwong, Allison Powell, Troy Patterson, Ella Liberzon, Richard Decker, Julio Gomez Rodriguez, Darby Kreienberg, Jennifer Bian, Urmi Patankar, Holly Horton, Jian Ding, Robert Hofmeister, Dario Gutierrez, Robert Tighe
Publikováno v:
Cancer Research. 82:557-557
We have previously described the generation of autologous T Cell Receptor Fusion Construct (TRuC™) T cells which are engineered to express a fusion protein comprised of an antibody-derived binder tethered to the extracellular domain of the CD3ε si
Autor:
Kendell Clement, Hans R. Schöler, Alexandra L. Mattei, Elena K. Stamenova, Julie Donaghey, Andreas Gnirke, Hongcang Gu, Alexander Meissner, Bernd Timmermann, Christina Galonska, Arman W. Mohammad, Jocelyn Charlton, Tobias Cantz, Sven Klages, Michael J. Ziller, Davide Cacchiarelli
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-9 (2018)
Nature Communications
Nature Communications
In normal mammalian development cytosine methylation is essential and is directed to specific regions of the genome. Despite notable advances through mapping its genome-wide distribution, studying the direct contribution of DNA methylation to gene an
Autor:
Julie Donaghey, Kendell Clement, Jennifer S. Chen, Andreas Gnirke, Casey A. Gifford, John L. Rinn, Michael J. Ziller, Ramona Pop, Rahul Karnik, Elena K. Stamenova, Jocelyn Charlton, David R. Kelley, Sudhir Thakurela, Hongcang Gu, Alexander Meissner, Zachary D. Smith, Davide Cacchiarelli
Publikováno v:
Nature Genetics
Transcription factors (TFs) direct developmental transitions by binding to target DNA sequences, influencing gene expression and establishing complex gene-regultory networks. To systematically determine the molecular components that enable or constra
Autor:
Dario A. Gutierrez, Holly M. Horton, Troy Patterson, Robert Hofmeister, Julie Donaghey, Philippe Kieffer-Kwon, Jessica Gierut, Ryan Milione, Julio Gomez-Rodriguez, Robert Tighe, Tiffany Chan, Joshua Daniel
Publikováno v:
Cancer Research. 81:1514-1514
The natural T cell receptor (TCR) recognizes its cognate peptide antigen only when presented on human leukocyte antigen (HLA) molecules and requires HLA matching of α/β TCR-engineered T cells for cancer therapy. To bypass the need for HLA matching,
Autor:
Julie Donaghey, Holly M. Horton, Robert Hofmeister, Philippe Kieffer-Kwon, Robert Tighe, Tiffany Chan, Troy Patterson, Dario A. Gutierrez, Daniel R. Getts
Publikováno v:
Cancer Research. 80:2190-2190
The T-Cell Receptor (TCR) can be functionally split into antigen recognition mediated by the TCRα/β heterodimer and signal transduction triggered by the CD3 complex comprising CD3ϵ/γ, CD3ϵ/δ and CD3ζ/ζ dimers. Unlike antibodies, the TCR recog
Autor:
Jocelyn Charlton, Julie Donaghey, Juan R. Alvarez-Dominguez, Aharon Helman, Niloofar Rasouli, Alexander Meissner, Douglas A. Melton, Juerg R. Straubhaar, Jennifer H. R. Kenty
Publikováno v:
Cell Stem Cell
Summary Stem-cell-derived tissues could transform disease research and therapy, yet most methods generate functionally immature products. We investigate how human pluripotent stem cells (hPSCs) differentiate into pancreatic islets in vitro by profili
Autor:
Kendell Clement, Andreas Gnirke, Alexander M. Tsankov, J. Keith Joung, Christina Galonska, Casey A. Gifford, Julie Donaghey, Rahul Karnik, Shengdar Q. Tsai, William Mallard, Veronika Akopian, John L. Rinn, Ramona Pop, Jing Liao, Michael J. Ziller, Hongcang Gu, Alexander Meissner, Deepak Reyon
Publikováno v:
Nature Genetics. 47:469-478
DNA methylation is a key epigenetic modification involved in regulating gene expression and maintaining genomic integrity. Here we inactivated all three catalytically active DNA methyltransferases (DNMTs) in human embryonic stem cells (ESCs) using CR
Autor:
Hongcang Gu, Alexander Meissner, Alexander M. Tsankov, Veronika Akopian, Andreas Gnirke, Michael J. Ziller, Julie Donaghey, Ido Amit
Publikováno v:
Nature
Summary Pluripotent stem cells provide a powerful system to dissect the underlying molecular dynamics that regulate cell fate changes during mammalian development. Here we report the integrative analysis of genome wide binding data for 38 transcripti