Aquaporin-Based Biomimetic Polymeric Membranes: Approaches and Challenges

Autor: Joachim Habel, Michael Hansen, Søren Kynde, Nanna Larsen, Søren Roi Midtgaard, Grethe Vestergaard Jensen, Julie Bomholt, Anayo Ogbonna, Kristoffer Almdal, Alexander Schulz, Claus Hélix-Nielsen

Publikováno v: Membranes, Vol 5, Iss 3, Pp 307-351 (2015)

In recent years, aquaporin biomimetic membranes (ABMs) for water separation have gained considerable interest. Although the first ABMs are commercially available, there are still many challenges associated with further ABM development. Here, we discu

Externí odkaz: https://doaj.org/article/8afb345ba5f943e4aac4212ad6569c40

Purification and functional characterization of nine human Aquaporins produced in Saccharomyces cerevisiae for the purpose of biophysical characterization

Autor: Claus Hélix-Nielsen, Kamil Gotfryd, Frederik Bühring Bjørkskov, Pontus Gourdon, Karen Molbaek, Per Amstrup Pedersen, Julie Bomholt, Casper Normann Nurup, Julie Winkel Missel, Simon Krabbe, Mariana Spulber

Publikováno v: Pedersen, P A, Gourdon, P E, Gotfryd, K, Hansen, K M, Hélix-Nielsen, C, Bomholt, J, Spulber, M, Missel, J W, Bühring Bjørkskov, F, Lyngaa Krabbe, S & Nurup, C N 2017, ' Purification and functional characterization of nine human Aquaporins produced in Saccharomyces cerevisiae for the purpose of biophysical characterization ', Scientific Reports, vol. 7, 16899 . https://doi.org/10.1038/s41598-017-17095-6
Scientific Reports
Scientific Reports, Vol 7, Iss 1, Pp 1-21 (2017)
Bjørkskov, F B, Krabbe, S, Nurup, C N, Missel, J W, Spulber, M, Bomholt, J, Molbaek, K, Helix-Nielsen, C, Gotfryd, K, Gourdon, P E & Pedersen, P A 2017, ' Purification and functional comparison of nine human Aquaporins produced in Saccharomyces cerevisiae for the purpose of biophysical characterization ', Scientific Reports, vol. 7, 16899 . https://doi.org/10.1038/s41598-017-17095-6

The sparse number of high-resolution human membrane protein structures severely restricts our comprehension of molecular physiology and ability to exploit rational drug design. In the search for a standardized, cheap and easily handled human membrane

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5e9e346203e55fd3b7621229615c639b
https://orbit.dtu.dk/en/publications/bf37cf23-c314-4f4f-9314-815a6020c70a

Monitoring the Aggregation of Single Casein Micelles Using Fluorescence Microscopy

Autor: Dimitrios Stamou, Karsten B. Qvist, Julie Bomholt, Thomas Bjørnholm, Anders O Karlson, Marianne Kirsten Harboe, Kasper Moth-Poulsen

Publikováno v: Langmuir. 27:866-869

The aggregation of casein micelles (CMs) induced by milk-clotting enzymes is a process of fundamental importance in the dairy industry for cheese production; however, it is not well characterized on the nanoscale. Here we enabled the monitoring of th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a1b1dd215b57df8ff901e68c9bf5ddc8
https://doi.org/10.1021/la1035163

Comparative Neural Network and Alignment Study of Aquaporins

Autor: Jesper S. Hansen, Claus Hélix-Nielsen, Julie Bomholt, Per Jr Greisen, Dan A. Klaerke

Publikováno v: Biophysical Journal. 100(3)

Common for the membrane channel protein family of aquaporins is that they facilitate a rapid transport of water molecules. Aquaporins are found in a wide range of organisms and several protein structures have been solved. Although they share structur

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c47785e51763db59ca24769128f1227e

Interaction between sodium dodecyl sulfate and membrane reconstituted aquaporins: A comparative study of spinach SoPIP2;1 and E. coli AqpZ

Autor: Jaume Torres, Ardcharaporn Vararattanavech, Jesper S. Hansen, I Plasencia, Per Jr Greisen, Julie Bomholt, Claus Hélix-Nielsen, Jenny Emnéus

Publikováno v: Biochimica et Biophysica Acta (BBA) - Biomembranes. (10):2600-2607

This study describes the interaction between sodium dodecyl sulfate (SDS) and membrane proteins reconstituted into large unilamellar lipid vesicles and detergent micelles studied by circular dichroism (CD) and polarity sensitive probe labeling. Speci

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::79ac667633af13e75ef26ee04d005a2b