Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Julian A. J. Hanak"'
We report the construction of two novel Escherichia coli strains (DH1lacdapD and DH1lacP2dapD) that facilitate the antibiotic-free selection and stable maintenance of recombinant plasmids in complex media. They contain the essential chromosomal gene,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::11a8e3aac7ceea241b3e6552dcd0e6b3
https://doi.org/10.1093/nar/29.5.e26
https://doi.org/10.1093/nar/29.5.e26
Autor:
Olga Durany, Rocky M. Cranenburgh, Philip Bassett, Carles de Mas, Julian A. J. Hanak, Pau Ferrer, Amanda Weiss, Josep López-Santín
Publikováno v:
Biotechnology and Bioengineering. 91:460-467
We report a novel application for the operator-repressor titration (ORT) plasmid maintenance system. The ability of ORT to maintain a plasmid during production of DNA has been demonstrated previously. In this study, we have used the ORT system to mai
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::871e3145b30d77d7f67b349e26b1b971
https://doi.org/10.1002/bit.20527
https://doi.org/10.1002/bit.20527
Publikováno v:
Microbial Physiology. 7:197-203
The Escherichia coli strain DH1lacdapD enables plasmid selection and maintenance that is free from antibiotics and selectable marker genes. This is achieved by using only the lac operator sequence as a selectable element. This strain is currently use
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6b510137c0939e981dce25f4dfbe67ad
https://doi.org/10.1159/000079828
https://doi.org/10.1159/000079828
Autor:
Alvin W. Nienow, H. Patel, Julian A. J. Hanak, Anthony Gordon Crewe Hitchcock, Saethawat Chamsart
Publikováno v:
Biotechnology and Bioengineering. 75:387-392
Extensive tests have been carried out to assess the impact of fluid-dynamic-generated stress during alkaline lysis of Escherichia coli cells (host strain DH1 containing the plasmid pTX 0161) to produce a plasmid DNA (pDNA) solution for gene therapy.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::35a8c98e16228f87c620644944bd5522
https://doi.org/10.1002/bit.10046
https://doi.org/10.1002/bit.10046
Autor:
Helen S. Garmory, Rocky M. Cranenburgh, Kate F. Griffin, Matthew W. Leckenby, Stephen J. Elvin, Julian A. J. Hanak, Rosa R. Taylor, E. Diane Williamson, M. Gill Hartley
Live, attenuated bacteria are effective vectors for heterologous antigen delivery. However, loss of heterologous gene-bearing plasmids is problematic, and antibiotics and their resistance genes are not desirable for in vivo DNA vaccine delivery due t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::18a4a67cfd36df5ac8bb80527e0f6fc9
https://europepmc.org/articles/PMC1087430/
https://europepmc.org/articles/PMC1087430/
Publikováno v:
Journal of molecular microbiology and biotechnology. 7(4)
The Escherichia coli strain DH1lacdapD enables plasmid selection and maintenance that is free from antibiotics and selectable marker genes. This is achieved by using only the lac operator sequence as a selectable element. This strain is currently use
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::8ea345d26bd11117689d76830f1ea2d5
https://pubmed.ncbi.nlm.nih.gov/15383717
https://pubmed.ncbi.nlm.nih.gov/15383717
Publikováno v:
Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology ISBN: 9789048157563
The use of antibiotics and antibiotic resistance genes is rapidly becoming unacceptable in many areas of biotechnology, such as recombinant protein production, engineering of transgenic organisms, DNA vaccine and gene therapy applications. Plasmid-bo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4939350df750bc169beeca57a4e30e34
https://doi.org/10.1007/978-94-015-9749-4_9
https://doi.org/10.1007/978-94-015-9749-4_9
Autor:
Christopher J. Wrighton, Amanda Weiss, Julian A. J. Hanak, Rocky M. Cranenburgh, David J. Sherratt, Steven Geraint Williams
Publikováno v:
Nucleic acids research. 26(9)
The propagation of recombinant plasmids in bacterial hosts, particularly in Escherichia coli, is essential for the amplification and manipulation of cloned DNA and the production of recombinant proteins. The isolation of bacterial transformants and s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b156ce85538ee2521ff49649aa0975e1
https://pubmed.ncbi.nlm.nih.gov/9547269
https://pubmed.ncbi.nlm.nih.gov/9547269
Autor:
Christina Herzberg, Toshiaki Muto, Fernando Sesma, Hideaki Yukawa, Julian A. J. Hanak, Kathryn S. Lewis, Sergio E. Pasteris, Petra Ehrenreich, Heiko Liesegang, Haruo Ishikawa, Anke Henne, Armin Ehrenreich, Bruno Goddeeris, Eric Cox, María de Fátima Alvarez, Gerhard Gottschalk, Shohei Okino, Alain A. Vertès, Sebastian Bäumer, Rocky M. Cranenburgh, Roxana Beatriz Medina, Rainer Merkl, Masahiro Yasuda, Miharu Matsumura, Karl Heinz Maurer, Kosaku Ishimi, Silke Steckel, Takashi Mimitsuka, Jörg Feesche, Shikiko Murakami, Hideo Kawaguchi, Birgit Veith, Hiroyasu Ogino, Ana M. Strasser de Saad, Masayuki Inui, Frank Verdonck
Publikováno v:
Journal of Molecular Microbiology and Biotechnology. 7:225-226
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::754622621b9d91951b4c719705f351ab
https://doi.org/10.1159/000080974
https://doi.org/10.1159/000080974