Dried urine spots - A novel sampling technique for comprehensive LC-MSn drug screening

Autor: Julian A. Michely, Hans H. Maurer, Markus R. Meyer

Publikováno v: Analytica Chimica Acta. 982:112-121

Dried matrix spot (DMS) technique as alternative sampling strategy, especially dried urine spots (DUS), might be an alternative for drug screening. So far only particular drugs or drug classes were covered in DMS screenings. Therefore, workup of DUS

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::e814c742901884dca4d8736602efe12a
https://doi.org/10.1016/j.aca.2017.05.033

LC-HR-MS/MS standard urine screening approach: Pros and cons of automated on-line extraction by turbulent flow chromatography versus dilute-and-shoot and comparison with established urine precipitation

Autor: Julian A. Michely, Markus R. Meyer, Armin A. Weber, Hans H. Maurer, Andreas G. Helfer

Publikováno v: Journal of Chromatography B. 1043:138-149

Comprehensive urine screening for drugs and metabolites by LC-HR-MS/MS using Orbitrap technology has been described with precipitation as simple workup. In order to fasten, automate, and/or simplify the workup, on-line extraction by turbulent flow ch

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5812db63153230428dbb9a66f9ff0a70
https://doi.org/10.1016/j.jchromb.2016.06.036

Refined protocols of tamoxifen injection for inducible DNA recombination in mouse astroglia

Autor: Alexei Verkhratsky, Hannah M. Jahn, Carmen V. Kasakow, Andreas G. Helfer, Anja Scheller, Hans H. Maurer, Julian A. Michely, Frank Kirchhoff

Publikováno v: Scientific Reports
Jahn, H M, Kasakow, C V, Helfer, A, Michely, J, Verkhratsky, A, Maurer, H H, Scheller, A & Kirchhoff, F 2018, ' Refined protocols of tamoxifen injection for inducible DNA recombination in mouse astroglia ', Scientific Reports, vol. 8, no. 1, 5913 . https://doi.org/10.1038/s41598-018-24085-9
Scientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)

Inducible DNA recombination of floxed alleles in vivo by liver metabolites of tamoxifen (TAM) is an important tool to study gene functions. Here, we describe protocols for optimal DNA recombination in astrocytes, based on the GLAST-CreERT2/loxP syste

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a3511c710ce48661171a414bc4ede9e6
https://doi.org/10.1038/s41598-018-24085-9

Paper Spray Ionization Coupled to High Resolution Tandem Mass Spectrometry for Comprehensive Urine Drug Testing in Comparison to Liquid Chromatography-Coupled Techniques after Urine Precipitation or Dried Urine Spot Workup

Autor: Julian A. Michely, Hans H. Maurer, Markus R. Meyer

Publikováno v: Analytical chemistry. 89(21)

Screening procedures using high resolution (HR)-mass spectrometry (MS) are getting more and more important, e.g., for drug testing or adherence monitoring. Approaches usually include time-consuming sample preparation and compound separation by liquid

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::831bf4c83882d6595b434decb5283438
https://pubmed.ncbi.nlm.nih.gov/29022692

A multi-analyte approach to help in assessing the severity of acute poisonings - Development and validation of a fast LC-MS/MS quantification approach for 45 drugs and their relevant metabolites with one-point calibration

Autor: Julian A. Michely, Hans H. Maurer

Publikováno v: Drug testing and analysis. 10(1)

Diagnosis, monitoring of the efficiency of detoxification, and estimating the prognosis of acute poisonings are important tasks in emergency toxicology. Comprehensive screening and quantification of relevant substances by gas chromatography-mass spec

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8cdd74426f8e57894bef2345ae3628ff
https://pubmed.ncbi.nlm.nih.gov/28777878