Výsledky vyhledávání - "Julia Golova"

Lab-on-a-Film disposable for genotyping multidrug-resistant Mycobacterium tuberculosis from sputum extracts

Autor: Yvonne Linger, Rebecca C. Holmberg, Christopher G. Cooney, Alex Perov, Thomas Sebastian, Alexander C Kukhtin, Arial Bueno, Peter Qu, Julia Golova, Christopher Knickerbocker, Michael Villanueva, Darrell P. Chandler

Publikováno v: Lab on a chip. 19(7)

We describe a Lab-on-a-Film disposable that detects multidrug-resistant tuberculosis (MDR-TB) from sputum extracts. The Lab-on-a-Film disposable consists of 203 gel elements that include DNA sequences (probes) for 37 mutations, deletions, or insertio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::82fd3bdc6580aaf8a086fb5006538f67
https://pubmed.ncbi.nlm.nih.gov/30801596

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Genotyping Multidrug-Resistant Mycobacterium tuberculosis from Primary Sputum and Decontaminated Sediment with an Integrated Microfluidic Amplification Microarray Test

Autor: Molly F. Franke, Nitu Thakore, Peter Qu, Megan Murray, Yvonne Linger, Rebecca C. Holmberg, Leonid Lecca, Roger Calderon, Christopher Knickerbocker, Alexander Kukhtin, Darrell P. Chandler, Julia Golova, David Sipes, Christopher G. Cooney

Publikováno v: Journal of Clinical Microbiology

There is a growing awareness that molecular diagnostics for detect-to-treat applications will soon need a highly multiplexed mutation detection and identification capability. In this study, we converted an open-amplicon microarray hybridization test

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::668b5e0ea04f3e697420c1423aef6ecb
https://doi.org/10.1128/jcm.01652-17

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Development and clinical testing of a simple, low-density gel element array for influenza identification, subtyping, and H275Y detection

Autor: Christopher Knickerbocker, Cory Wiles, Amine Lambarqui, Cynthia Zimmerman, Kirsten St. George, Sara B. Griesemer, Alexander Perov, George B. Rudy, Darrell P. Chandler, Julia Golova

Publikováno v: Journal of Virological Methods. 208:152-159

The objectives of this study were to develop a user-friendly, gel element microarray test for influenza virus detection, subtyping, and neuraminidase inhibitor resistance detection, assess the performance characteristics of the assay, and perform a c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::744e827cecc1d978da96e86bd2173c76
https://doi.org/10.1016/j.jviromet.2014.07.019

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Simplified Microarray System for Simultaneously Detecting Rifampin, Isoniazid, Ethambutol, and Streptomycin Resistance Markers in Mycobacterium tuberculosis

Autor: Lexi Bryant, Yvonne Linger, Darrell P. Chandler, Stefanie L. Fisher, Alexander Perov, Amine Lambarqui, Alexander Kukhtin, Kim Dionne, Julia Golova, George B. Rudy, Nicole Parrish

Publikováno v: Journal of Clinical Microbiology. 52:2100-2107

We developed a simplified microarray test for detecting and identifying mutations in rpoB , katG , inhA , embB , and rpsL and compared the analytical performance of the test to that of phenotypic drug susceptibility testing (DST). The analytical sens

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3a7bccaadcafb0433b8647a5cfecbc6a
https://doi.org/10.1128/jcm.00238-14

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Nonvolatile copolymer compositions for fabricating gel element microarrays

Autor: Yvonne Linger, Alexander Kukhtin, Jennifer Reynolds, Alexander Perov, Darrell P. Chandler, Julia Golova, Boris K. Chernov

Publikováno v: Analytical Biochemistry. 421:526-533

By modifying polymer compositions and cross-linking reagents, we have developed a simple yet effective manufacturing strategy for copolymerized three-dimensional gel element arrays. A new gel-forming monomer (2-(hydroxyethyl) methacrylamide; HEMAA) w

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::06191796815327b2ba93d26489532d33
https://doi.org/10.1016/j.ab.2011.09.028

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Suspension Array Analysis of 16S rRNA from Fe- and SO 4 2 -Reducing Bacteria in Uranium-Contaminated Sediments Undergoing Bioremediation

Autor: Philip E. Long, Boris Chernov, Ann E. Jarrell, Aaron D. Peacock, Julia Golova, Matthew J. Schipma, Darrell P. Chandler, Eric R. Roden

Publikováno v: Applied and Environmental Microbiology. 72:4672-4687

A 16S rRNA-targeted tunable bead array was developed and used in a retrospective analysis of metal- and sulfate-reducing bacteria in contaminated subsurface sediments undergoing in situ U(VI) bioremediation. Total RNA was extracted from subsurface se

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::682f6c4c6d71ee5cf4fdec4b8bd3c726
https://doi.org/10.1128/aem.02858-05

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Diagnostic Oligonucleotide Microarray Fingerprinting of Bacillus Isolates

Autor: Miroslava Protic, Alan Willse, Oleg Alferov, Boris Chernov, Don S. Daly, Amanda M. White, Julia Golova, Richard A. Robison, Alexander Perov, Darrell P. Chandler, Matthew J. Schipma

Publikováno v: Journal of Clinical Microbiology. 44:244-250

A genome-independent microarray and new statistical techniques were used to genotype Bacillus strains and quantitatively compare DNA fingerprints with the known taxonomy of the genus. A synthetic DNA standard was used to understand process level vari

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f03d593971253285d90f769aeb520b8a
https://doi.org/10.1128/jcm.44.1.244-250.2006

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Demonstrating a multi-drug resistant Mycobacterium tuberculosis amplification microarray

Autor: Yvonne, Linger, Alexander, Kukhtin, Julia, Golova, Alexander, Perov, Peter, Qu, Christopher, Knickerbocker, Christopher G, Cooney, Darrell P, Chandler

Publikováno v: Journal of visualized experiments : JoVE. (86)

Simplifying microarray workflow is a necessary first step for creating MDR-TB microarray-based diagnostics that can be routinely used in lower-resource environments. An amplification microarray combines asymmetric PCR amplification, target size selec

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::ead1267a4995222945b5402c7dd450f9
https://pubmed.ncbi.nlm.nih.gov/24796567

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Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray

Autor: Julia Golova, Alexander Kukhtin, Yvonne Linger, Christopher Knickerbocker, Alexander Perov, Christopher G. Cooney, Peter Qu, Darrell P. Chandler

Publikováno v: Journal of Visualized Experiments.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1c4a9e1da6c532515e5c82a73e5ab648
https://doi.org/10.3791/51256

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