Point mutations in the PDX1 transactivation domain impair human β-cell development and function

Autor: Xianming Wang, Michael Sterr, Ansarullah, Ingo Burtscher, Anika Böttcher, Julia Beckenbauer, Johanna Siehler, Thomas Meitinger, Hans-Ulrich Häring, Harald Staiger, Filippo M. Cernilogar, Gunnar Schotta, Martin Irmler, Johannes Beckers, Christopher V.E. Wright, Mostafa Bakhti, Heiko Lickert

Publikováno v: Molecular Metabolism, Vol 24, Iss , Pp 80-97 (2019)

Objective: Hundreds of missense mutations in the coding region of PDX1 exist; however, if these mutations predispose to diabetes mellitus is unknown. Methods: In this study, we screened a large cohort of subjects with increased risk for diabetes and

Externí odkaz: https://doaj.org/article/38c7550f0463430bba653e3eba240c45

Generation of a human induced pluripotent stem cell line (HMGUi002-A) from a healthy male individual

Autor: Xianming Wang, Andrzej R. Malinowski, Julia Beckenbauer, Johanna Siehler, Anna Blöchinger, Thomas Meitinger, Hans-Ulrich Häring, Harald Staiger, Ingo Burtscher, Heiko Lickert

Publikováno v: Stem Cell Research, Vol 39, Iss , Pp - (2019)

Induced pluripotent stem cells (iPSCs) can be used to generate different somatic cell types in vitro, including insulin-producing pancreatic β-cells. Here, we have generated iPSCs from a healthy male individual using an episomal reprogramming method

Externí odkaz: https://doaj.org/article/f72f05a930a24fe49056510d3ca4be9a

Generation of a novel nkx6-1 venus fusion reporter mouse line

Autor: Julia Beckenbauer, Marta Tarquis-Medina, Ciro Salinno, Mostafa Bakhti, Heiko Lickert, Silvia Schirge, Ingo Burtscher

Publikováno v: Int. J. Mol. Sci. 22:3434 (2021)
International Journal of Molecular Sciences, Vol 22, Iss 3434, p 3434 (2021)
International Journal of Molecular Sciences
Volume 22
Issue 7

Nkx6-1 is a member of the Nkx family of homeodomain transcription factors (TFs) that regulates motor neuron development, neuron specification and pancreatic endocrine and β-cell differentiation. To facilitate the isolation and tracking of Nkx6-1-exp

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7c31310d6151162877364023bbb23d14
https://push-zb.helmholtz-muenchen.de/frontdoor.php?source_opus=61714

Generation of a homozygous ARX nuclear CFP (ARX) reporter human iPSC line (HMGUi001-A-4)

Autor: Noel Moya, Mostafa Bakhti, Julia Beckenbauer, Heiko Lickert, Alireza Shahryari, Ingo Burtscher

Publikováno v: Stem Cell Research, Vol 46, Iss, Pp 101874-(2020)

The aristaless related homeobox (ARX) transcription factor plays a crucial role in glucagon-producing α-cell differentiation. Here, we generate an ARX reporter iPSC line by 3′ fusion of an intervening viral T2A sequence followed by a nuclear-local

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::50050dbf0c55debd52de2cbc5eda800f
https://mediatum.ub.tum.de/1600920

Generation of pancreatic β cells from CD177

Autor: Pallavi U, Mahaddalkar, Katharina, Scheibner, Sandra, Pfluger, Ansarullah, Michael, Sterr, Julia, Beckenbauer, Martin, Irmler, Johannes, Beckers, Sebastian, Knöbel, Heiko, Lickert

Publikováno v: Nature biotechnology. 38(9)

Methods for differentiating human pluripotent stem cells to pancreatic and liver lineages in vitro have been limited by the inability to identify and isolate distinct endodermal subpopulations specific to these two organs. Here we report that pancrea

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::d8b8f710d35443423d9e6404e3612a24
https://pubmed.ncbi.nlm.nih.gov/32341565