Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Juergen Alves"'
Publikováno v:
Journal of Biological Chemistry. 277:8209-8216
Epsin and AP180/CALM are important endocytic accessory proteins that are believed to be involved in the formation of clathrin coats. Both proteins associate with phosphorylated membrane inositol lipids through their epsin N-terminal homology domains
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f018fc7e9776a3c1bac97c3ee1f47bd0
https://doi.org/10.1074/jbc.m111587200
https://doi.org/10.1074/jbc.m111587200
Autor:
Stephanie Windolph, Juergen Alves
Publikováno v:
European journal of biochemistry. 244(1)
To understand the functional role of the inner arm region of EcoRI for the interaction with its DNA substrate, we mutated each of the positively charged amino acid residues Lys130 and Arg131 to Ala or Glu. The resulting cleavage activities are only a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ddf98e9da6957b34aeeba1f304de611c
https://pubmed.ncbi.nlm.nih.gov/9063456
https://pubmed.ncbi.nlm.nih.gov/9063456
Publikováno v:
Biochemistry. 34(35)
In order to investigate the accuracy of the EcoRV restriction endonuclease, we have synthesized a set of double-stranded oligodeoxynucleotides comprising the canonical recognition sequence, the 9 star sequences (i.e., sequences deviating by one base
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e30c1374c059a6546f8eb4fdfaa4e38e
https://pubmed.ncbi.nlm.nih.gov/7669777
https://pubmed.ncbi.nlm.nih.gov/7669777
Publikováno v:
Biochemistry. 33(34)
Linear diffusion is a mechanism to accelerate association rates beyond their three-dimensional diffusional limit. It is employed by the restriction endonuclease EcoRI as well as many other proteins interacting with specific DNA sequences to locate th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad9003b38420f26f2abad89fe4f5699d
https://pubmed.ncbi.nlm.nih.gov/8068662
https://pubmed.ncbi.nlm.nih.gov/8068662
Publikováno v:
Biochemistry. 29(19)
We have synthesized a series of 18 nonpalindromic oligodeoxynucleotides that carry all possible base changes within the recognition sequence of EcoRI. These single strands can be combined with their complementary single strands to obtain all possible
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::076d039dc9015e0f386512af62b3851a
https://pubmed.ncbi.nlm.nih.gov/2372551
https://pubmed.ncbi.nlm.nih.gov/2372551
Publikováno v:
Biochemistry. 28:7879-7888
We have investigated in fluorescence stopped-flow and temperature-jump experiments the EcoRI-catalyzed cleavage of synthetic palindromic tridecadeoxynucleotides which contain the EcoRI site but differ in the flanking sequences. The overall reaction c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::384adb8a66bdb9a00e75d97447cd7948
https://doi.org/10.1021/bi00445a050
https://doi.org/10.1021/bi00445a050
Autor:
Claus Urbanke, Hans Juergen Ehbrecht, Juergen Alves, Claudio O. Gualerzi, Alfred Pingoud, Marc Zabeau
Publikováno v:
Biochemistry. 23:5697-5703
We have investigated the effect of the polyamines spermine, spermidine, and putrescine and the prokaryotic histone-like proteins NS1 and NS2 on the restriction endonuclease EcoRI catalyzed cleavage of plasmid and bacteriophage DNAs. At low concentrat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bc07780381c51015ff3894b46c1baafa
https://doi.org/10.1021/bi00319a006
https://doi.org/10.1021/bi00319a006
Autor:
Rüter T, Anja Fliess, Heiner Wolfes, Juergen Alves, Pingoud, Düsterhöft A, Geiger R, Günter Maass, A. Pingoud, Claus Urbanke
Publikováno v:
Biochemistry. 28:2667-2677
We have genetically engineered the Arg200----Lys mutant, the Glu144Arg145----GlnLys double mutant, and the Glu144Arg145Arg200----GlnLysLys triple mutant of the EcoRI endonuclease in extension of previously published work on site-directed mutagenesis
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::71ec74434607c39403b4c50bb1f53a3b
https://doi.org/10.1021/bi00432a046
https://doi.org/10.1021/bi00432a046
Autor:
Alfred Wittinghofer, Juergen Alves, Frank Ulrich Gast, Mathias Wehrmann, Alfred Pingoud, Claus Urbanke, Uwe Pieper, Juergen Feuerstein
Publikováno v:
Biochemistry
We have recorded the circular dichroism spectra of the cellular and the viral H-ras gene products both in the absence and in the presence of guanine nucleotides and analyzed these spectra in terms of the secondary structure composition of these prote
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::761b6fac03f3817889f8a0de603f6355
https://pubmed.ncbi.nlm.nih.gov/3048389/
https://pubmed.ncbi.nlm.nih.gov/3048389/
Publikováno v:
Biophysics of Structure and Mechanism. 7:247-248
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7bd33da0923fff373cba62981d43bfc9
https://doi.org/10.1007/bf02425379
https://doi.org/10.1007/bf02425379