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pro vyhledávání: '"Judith A. Snyder"'
Autor:
Rollin W. Robinson, Judith A. Snyder
Publikováno v:
Protoplasma. 225:113-122
The enzymes of importance in moving chromosomes are called motor proteins and include dynein, kinesin, and possibly myosin II. These three molecules are all included in the category of ATPases, in that they have the ability to convert chemical energy
Autor:
Judith A. Snyder, Rollin W. Robinson
Publikováno v:
Cell Biology International. 27:665-673
In this study we extend our analysis of the effect of Cytochalasin J (CJ) on mitotic and interphase cells by the use of immunocytochemical techniques to localize antigens to anti-beta-tubulin, anti-dynein heavy chain (HC), anti-dynein intermediate ch
Autor:
Judith A. Snyder, Gregory A. Wrench
Publikováno v:
Cell Biology International. 25:815-820
Mitotic PtK1 cells were arrested in mitosis with nocodazole to determine the effect of cytochalasin J (CJ) on kinetochore structure in arrested and nocodazole-released cells. In previous studies it was shown that CJ had a more pronounced effect on al
Autor:
Judith A. Snyder, Gregory A. Wrench
Publikováno v:
Cell Motility and the Cytoskeleton. 36:112-124
It has previously been demonstrated that treatment of mitotic PtK1 cells with 10-20 microg/ml cytochalasin J (CJ) blocks or slows chromosome motion and has a significant effect on spindle architecture [Snyder and Cohen, 1995: Cell Motil. Cytoskeleton
Autor:
Judith A. Snyder, Sandra L. Mclelland
Publikováno v:
Cell Biology International. 20:573-578
Mitotic PtK1 cells were treated both during mid-anaphase and at furrow initiation with the potent microtubule (MT) stabilizing agent, taxol, to determine the role of MTs in the rate of cytokinetic events. Rates of cytokinesis (micron/min) were measur
Publikováno v:
Protoplasma. 186:193-200
Chinese hamster ovary (CHO) cells are treated with hydroxurea followed by a caffeine treatment to form detached kinetochore fragments in the absence of sister chromatids. Detached kinetochores in mitotic CHO cells display a functional association wit
Autor:
Judith A. Snyder, Laura Cohen
Publikováno v:
Cell Motility and the Cytoskeleton. 32:245-257
PtK1 cells were treated with 10 micrograms/ml cytochalasin J (CJ) for 15 min at various stages of mitosis. When applied at nuclear envelope breakdown (NEB) chromosome congression was blocked or substantially slowed, and chromosomes failed to show org
Autor:
Judith A. Snyder, David C. Geck
Publikováno v:
Cell Biology International. 18:1077-1084
Prometaphase PtK1 cells are treated with low concentrations of sucrose in order to analyze its effects on kinetochore structure, microtubule (MT) associations with the developing kinetochore and chromosome congression. Prometaphase cells treated with
Autor:
Judith A. Snyder, Mullins Jm
Publikováno v:
Cell Biology International. 17:1075-1084
Taxol, a microtubule stabilizing agent, has been used to study changes in spindle microtubule organization during mitosis. PtK1 cells have been treated with 5 micrograms/ml taxol for brief periods to determine its effect on spindle architecture. Duri
Role of microtubule organization in centrosome migration and mitotic spindle formation in PtK1 cells
Autor:
Lydia Armstrong, Judith A. Snyder
Publikováno v:
Protoplasma. 173:133-143
Quinacrine, an acridine derivative, has previously been shown to disrupt lateral associations between non-kinetochore microtubules (nkMTs) of opposite polarity in PtK1 metaphase spindles such that the balance of spindle forces is significantly altere