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Neuronal DNA repair reveals strategies to influence CRISPR editing outcomes.

Autor: Ramadoss GN; Gladstone Institutes, San Francisco, CA, 94158, USA.; Institute for Neurodegenerative Diseases, University of California, San Francisco, CA, 94158, USA., Namaganda SJ; Gladstone Institutes, San Francisco, CA, 94158, USA., Hamilton JR; Innovative Genomics Institute, University of California, Berkeley, CA, 94720, USA.; Department of Molecular & Cell Biology, University of California, Berkeley, CA, 94720, USA., Sharma R; Innovative Genomics Institute, University of California, Berkeley, CA, 94720, USA.; Department of Bioengineering, University of California, Berkeley, CA, 94720, USA., Chow KG; Gladstone Institutes, San Francisco, CA, 94158, USA., Macklin BL; Gladstone Institutes, San Francisco, CA, 94158, USA., Sun M; Gladstone Institutes, San Francisco, CA, 94158, USA., Liu JC; Laboratory of Genome Integrity, National Cancer Institute, NIH, Bethesda, MD, 20892, USA., Fellmann C; Gladstone Institutes, San Francisco, CA, 94158, USA.; Department of Cellular & Molecular Pharmacology, University of California, San Francisco, CA, 94158, USA., Watry HL; Gladstone Institutes, San Francisco, CA, 94158, USA., Jin J; Institute for Neurodegenerative Diseases, University of California, San Francisco, CA, 94158, USA., Perez BS; Innovative Genomics Institute, University of California, Berkeley, CA, 94720, USA.; Department of Molecular & Cell Biology, University of California, Berkeley, CA, 94720, USA., Sandoval Espinoza CR; Innovative Genomics Institute, University of California, Berkeley, CA, 94720, USA.; Department of Molecular & Cell Biology, University of California, Berkeley, CA, 94720, USA., Matia MP; Gladstone Institutes, San Francisco, CA, 94158, USA., Lu SH; Gladstone Institutes, San Francisco, CA, 94158, USA., Judge LM; Gladstone Institutes, San Francisco, CA, 94158, USA.; Department of Pediatrics, University of California, San Francisco, CA, 94158, USA., Nussenzweig A; Laboratory of Genome Integrity, National Cancer Institute, NIH, Bethesda, MD, 20892, USA., Adamson B; Department of Molecular Biology, Princeton University, Princeton, NJ, 08544, USA.; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, 08544, USA., Murthy N; Innovative Genomics Institute, University of California, Berkeley, CA, 94720, USA.; Department of Bioengineering, University of California, Berkeley, CA, 94720, USA., Doudna JA; Gladstone Institutes, San Francisco, CA, 94158, USA.; Innovative Genomics Institute, University of California, Berkeley, CA, 94720, USA.; Department of Molecular & Cell Biology, University of California, Berkeley, CA, 94720, USA.; California Institute for Quantitative Biosciences, University of California, Berkeley, CA, 94720, USA.; Howard Hughes Medical Institute, University of California, Berkeley, CA, 94720, USA.; Department of Chemistry, University of California, Berkeley, CA, 94720, USA.; MBIB Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA., Kampmann M; Institute for Neurodegenerative Diseases, University of California, San Francisco, CA, 94158, USA.; Department of Biochemistry & Biophysics, University of California, San Francisco, CA, 94158, USA., Conklin BR; Gladstone Institutes, San Francisco, CA, 94158, USA.; Innovative Genomics Institute, University of California, Berkeley, CA, 94720, USA.; Department of Cellular & Molecular Pharmacology, University of California, San Francisco, CA, 94158, USA.; Department of Medicine, University of California, San Francisco, CA, 94158, USA.

Publikováno v: BioRxiv : the preprint server for biology [bioRxiv] 2024 Jun 26. Date of Electronic Publication: 2024 Jun 26.

Bioengineered optogenetic model of human neuromuscular junction.

Autor: Vila OF; Columbia University, 622 W 168th St, New York, NY, 10032, USA; Gladstone Institutes, 1650 Owens St, San Francisco, CA, 94158, USA. Electronic address: olaia.vila@gladstone.ucsf.edu., Chavez M; Columbia University, 622 W 168th St, New York, NY, 10032, USA., Ma SP; Columbia University, 622 W 168th St, New York, NY, 10032, USA., Yeager K; Columbia University, 622 W 168th St, New York, NY, 10032, USA., Zholudeva LV; Gladstone Institutes, 1650 Owens St, San Francisco, CA, 94158, USA., Colón-Mercado JM; National Institute of Neurological Disorders and Stroke, NIH, Bethesda, MD, 20892, USA., Qu Y; Columbia University, 622 W 168th St, New York, NY, 10032, USA., Nash TR; Columbia University, 622 W 168th St, New York, NY, 10032, USA., Lai C; Gladstone Institutes, 1650 Owens St, San Francisco, CA, 94158, USA., Feliciano CM; Gladstone Institutes, 1650 Owens St, San Francisco, CA, 94158, USA; Department of Pediatrics, UCSF, 550 16th St, Floor 5, San Francisco, CA, 94143, USA., Carter M; Gladstone Institutes, 1650 Owens St, San Francisco, CA, 94158, USA., Kamm RD; Department of Mechanical Engineering and Biological Engineering, Massachusetts Institute of Technology, Cambridge MA, 02139, USA., Judge LM; Gladstone Institutes, 1650 Owens St, San Francisco, CA, 94158, USA; Department of Pediatrics, UCSF, 550 16th St, Floor 5, San Francisco, CA, 94143, USA., Conklin BR; Gladstone Institutes, 1650 Owens St, San Francisco, CA, 94158, USA., Ward ME; National Institute of Neurological Disorders and Stroke, NIH, Bethesda, MD, 20892, USA., McDevitt TC; Gladstone Institutes, 1650 Owens St, San Francisco, CA, 94158, USA., Vunjak-Novakovic G; Columbia University, 622 W 168th St, New York, NY, 10032, USA. Electronic address: gv2131@columbia.edu.

Publikováno v: Biomaterials [Biomaterials] 2021 Sep; Vol. 276, pp. 121033. Date of Electronic Publication: 2021 Jul 30.

Rapid, precise quantification of large DNA excisions and inversions by ddPCR.

Autor: Watry HL; Gladstone Institute of Data Sciences and Biotechnology, San Francisco, CA, USA.; Innovative Genomics Institute, Berkeley, CA, USA., Feliciano CM; Gladstone Institute of Data Sciences and Biotechnology, San Francisco, CA, USA.; Department of Pediatrics, UCSF, San Francisco, CA, USA., Gjoni K; Gladstone Institute of Data Sciences and Biotechnology, San Francisco, CA, USA., Takahashi G; Regenerative Medicine Project, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan., Miyaoka Y; Regenerative Medicine Project, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan., Conklin BR; Gladstone Institute of Data Sciences and Biotechnology, San Francisco, CA, USA. bconklin@gladstone.ucsf.edu.; Innovative Genomics Institute, Berkeley, CA, USA. bconklin@gladstone.ucsf.edu.; Department of Ophthalmology, UCSF, San Francisco, CA, USA. bconklin@gladstone.ucsf.edu.; Department of Medicine, UCSF, San Francisco, CA, USA. bconklin@gladstone.ucsf.edu., Judge LM; Gladstone Institute of Data Sciences and Biotechnology, San Francisco, CA, USA. luke.judge@gladstone.ucsf.edu.; Department of Pediatrics, UCSF, San Francisco, CA, USA. luke.judge@gladstone.ucsf.edu.

Publikováno v: Scientific reports [Sci Rep] 2020 Sep 10; Vol. 10 (1), pp. 14896. Date of Electronic Publication: 2020 Sep 10.