Zobrazeno 1 - 10
of 18
pro vyhledávání: '"Juan Perez-Vilar"'
Publikováno v:
Journal of Biological Chemistry. 281:4844-4855
Recent studies suggest that the mucin granule lumen consists of a matrix meshwork embedded in a fluid phase. Secretory products can both diffuse, although very slowly, through the meshwork pores and interact noncovalently with the matrix. Using a gre
Publikováno v:
Journal of Histochemistry & Cytochemistry. 53:1305-1309
Live cell imaging methods were used to characterize goblet cells expressing a MUC5AC domain fused to enhanced green fluorescent protein that labels the granule lumen. Golgi complex and endosome/lysosome elements largely resided in the periphery of th
Autor:
Juan Perez-Vilar, Anthony M. Paradiso, Lisa Jones, Ute E. Schwab, Carla Ribeiro, Richard C. Boucher, Wanda K. O'Neal
Publikováno v:
Journal of Biological Chemistry. 280:17798-17806
Hyperinflammatory responses to infection have been postulated as a component of cystic fibrosis (CF) lung disease. Studies have linked intracellular calcium (Ca(2+)(i)) mobilization with inflammatory responses in several systems. We have reported tha
Autor:
Juan Perez-Vilar, Robert L. Hill
Publikováno v:
Journal of Biological Chemistry. 273:34527-34534
Plasmids encoding the amino-terminal region of porcine submaxillary mucin were modified by site-specific mutagenesis to assess the roles of individual half-cystine residues in the assembly of disulfide-linked multimers of mucin. COS-7 cells with the
Autor:
Robert L. Hill, Juan Perez-Vilar
Publikováno v:
Journal of Biological Chemistry. 272:33410-33415
COS-7 cells transfected with a DNA construct encoding the 133 amino acids in norrin plus six histidine residues at its carboxyl terminus were pulse-labeled with [35S]cysteine, and the labeled norrin was examined in cell lysates, the medium, and the e
Autor:
Juan Perez-Vilar
Publikováno v:
Oral Delivery of Macromolecular Drugs ISBN: 9781441901996
A family of glycoproteins, known as gel-forming mucins, endow gastrointestinal mucus with its characteristic viscoelastic and biological properties. In the mucus, these large oligomeric glycoproteins are organized into entangled networks that occasio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::dc300d41df853102c3630bae47ab26e4
https://doi.org/10.1007/978-1-4419-0200-9_2
https://doi.org/10.1007/978-1-4419-0200-9_2
Autor:
Juan Perez-Vilar
Mucus secretions have played a central role in the evolution of multicellular organisms, enabling adaptation to widely differing environments. In vertebrates, mucus covers and protects the epithelial cells in the respiratory, gastrointestinal, urogen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce88552272e9456569a7ddd28e24b61b
https://europepmc.org/articles/PMC2176109/
https://europepmc.org/articles/PMC2176109/
Publikováno v:
The Journal of biological chemistry. 281(8)
Recent studies suggest that the mucin granule lumen consists of a matrix meshwork embedded in a fluid phase. Secretory products can both diffuse, although very slowly, through the meshwork pores and interact noncovalently with the matrix. Using a gre
Publikováno v:
The Journal of biological chemistry. 280(17)
To study the mechanism of gel-forming mucin packaging within mucin granules, we generated human mucous/goblet cells stably expressing a recombinant MUC5AC domain fused to green fluorescent protein (GFP). The fusion protein, named SHGFP-MUC5AC/CK, acc
Autor:
Juan Perez-Vilar, Richard C. Boucher
Publikováno v:
Free radical biologymedicine. 37(10)
The existence of mucus plugs, containing mucins, bacteria, and neutrophils, blocking the lower airways in the lung of cystic fibrosis (CF) patients has raised the possibility that production of "abnormal" mucins is a critical characteristic of this d