Zobrazeno 1 - 6
of 6
pro vyhledávání: '"Juan Miguel Puertas"'
Autor:
Nataša Božić, Josep López-Santín, Zoran Vujčić, Cristina Sans Duran, Juan Miguel Puertas, Nikola Lončar
Publikováno v:
Process Biochemistry
In this study, a new approach for extracellular production of recombinant alpha-amylase in Escherichia coli was investigated. A gene encoding a highly efficient raw-starch-digesting alpha-amylase from Bacillus licheniformis ATCC 9945a was cloned and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0586729f43aabaff5d805583bfefe060
https://doi.org/10.1016/j.procbio.2013.01.016
https://doi.org/10.1016/j.procbio.2013.01.016
Autor:
Brent L. Nannenga, François Baneyx, Kevin T. Dornfeld, Jean Michel Betton, Juan Miguel Puertas
Publikováno v:
Protein Expression and Purification. 74:122-128
The signal recognition particle (SRP) dependent secretion pathway is as an attractive alternative to Sec-dependent export for the production of disulfide-bonded and/or fast-folding recombinant proteins in the Escherichia coli periplasm. SRP, which sh
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::107bd4a792fa29581f718ceaa3d4a1dc
https://doi.org/10.1016/j.pep.2010.06.008
https://doi.org/10.1016/j.pep.2010.06.008
Autor:
Jordi Ruiz, Juan Miguel Puertas, Julia Lorenzo, Gloria Caminal, Glòria González, Mónica Rodríguez de la Vega
Publikováno v:
Process Biochemistry. 45:1334-1341
A high cell density cultivation protocol was developed for the secretory production of potato carboxypeptidase inhibitor (PCI) in Escherichia coli . The strain BW25113 (pIMAM3) was cultured in fed-batch mode employing minimal media and an exponential
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3ae2117796c79628dc5c3db85a84fb00
https://doi.org/10.1016/j.procbio.2010.04.024
https://doi.org/10.1016/j.procbio.2010.04.024
Publikováno v:
Journal of industrial microbiologybiotechnology. 38(9)
Metallocarboxypeptidase inhibitors are proteins with possible applications in biomedicine given their properties as anticoagulant and antitumoral factors. They are small, eukaryotic polypeptides comprising several disulfide bridges, which makes them
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d36eb568ebd50c7e7909a1c17d91761f
https://pubmed.ncbi.nlm.nih.gov/21301927
https://pubmed.ncbi.nlm.nih.gov/21301927
Autor:
Carles de Mas, Jaume Pinsach, Engràcia Costa, Juan Miguel Puertas, Glòria González, Pau Ferrer, Gloria Caminal, Cristina Sans, David Resina, Luis Vidal, Josep López-Santín
Publikováno v:
Journal of Biotechnology. 131:S137-S138
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::cbe495a1c3e3ae1e03a58de3af0da555
https://doi.org/10.1016/j.jbiotec.2007.07.839
https://doi.org/10.1016/j.jbiotec.2007.07.839
Publikováno v:
Microbial Cell Factories, Vol 8, Iss 1, p 57 (2009)
Microbial Cell Factories
Microbial Cell Factories, 2009, 8 (1), pp.57. ⟨10.1186/1475-2859-8-57⟩
Microbial Cell Factories, BioMed Central, 2009, 8 (1), pp.57. ⟨10.1186/1475-2859-8-57⟩
Microbial Cell Factories
Microbial Cell Factories, 2009, 8 (1), pp.57. ⟨10.1186/1475-2859-8-57⟩
Microbial Cell Factories, BioMed Central, 2009, 8 (1), pp.57. ⟨10.1186/1475-2859-8-57⟩
Background Despite advances in expression technologies, the efficient production of heterologous secreted proteins in Escherichia coli remains a challenge. One frequent limitation relies on their inability to be exported to the E. coli periplasm. How
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c7b2e6f8f4be731400ea518e465f06d4