Integration of phage and yeast display platforms: A reliable and cost effective approach for binning of peptides as displayed on-phage.

Autor: Priyanka Pandya, Robert O Sayers, Joey P Ting, Shaghayegh Morshedian, Carina Torres, Justine S Cudal, Kai Zhang, Jonathan R Fitchett, Qing Zhang, Feiyu F Zhang, Jing Wang, Jim D Durbin, Juan J Carrillo, Alfonso Espada, Howard Broughton, Yuewei Qian, Sepideh Afshar

Publikováno v: PLoS ONE, Vol 15, Iss 6, p e0233961 (2020)

Hundreds of target specific peptides are routinely discovered by peptide display platforms. However, due to the high cost of peptide synthesis only a limited number of peptides are chemically made for further analysis. Here we describe an accurate an

Externí odkaz: https://doaj.org/article/34b3432ac0334b28a6012c155f43114e

Deciphering Binding Interactions of IL-23R with HDX-MS: Mapping Protein and Macrocyclic Dodecapeptide Ligands

Autor: Cristina Sayago, Nathan C. Fite, Juan J. Carrillo, Feiyu F. Zhang, Isabel C. Gonzalez Valcarcel, Howard B. Broughton, Alfonso Espada, Michael J. Chalmers, Yuewei Qian, Jeffrey Alan Dodge, Jorge Alsina-Fernandez, John Lee

Publikováno v: ACS Medicinal Chemistry Letters. 9:912-916

[Image: see text] Molecular characterization of the binding epitope of IL-23R and its cognate cytokine IL-23 is paramount to understand the role in autoimmune diseases and to support the discovery of new inhibitors of this protein–protein interacti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b5cf4b8265a290d94779e6cf9d920e4c
https://doi.org/10.1021/acsmedchemlett.8b00255

Second-Harmonic Generation (SHG) for Conformational Measurements: Assay Development, Optimization, and Screening

Autor: Juan J. Carrillo, Ben Moree, John Strelow, Mary Geck Do, Joshua Salafsky, Tracy A. Young, Bason Clancy, Tarun Gheyi, Margaret T. Butko

Second-harmonic generation (SHG) has recently emerged as a biophysical tool for conformational sensing of a target biomolecule upon binding to ligands such as small molecules, fragments, proteins, peptides, and oligonucleotides. To date, SHG has been

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::bd85075312fdb7e806432d39d62ac79a
https://doi.org/10.1016/bs.mie.2018.09.017

Multiple Interactions between Transmembrane Helices Generate the Oligomeric α1b-Adrenoceptor

Autor: Graeme Milligan, Juan F. López-Giménez, Juan J. Carrillo

Publikováno v: Molecular Pharmacology. 66:1123-1137

Combinations of coimmunoprecipitation, single-cell fluorescence resonance energy transfer, and cell-surface time-resolved fluorescence resonance energy transfer demonstrated protein-protein interactions and quaternary structure for the alpha(1b)-adre

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0f009ceeebfcb8ba2bc2871d17e03039
https://doi.org/10.1124/mol.104.001586

Domain swapping in the human histamine H1 receptor

Autor: Graeme Milligan, Remko A. Bakker, Juan J. Carrillo, Juan F. López-Giménez, Philip G. Strange, Raymond G. Booth, Guido Dees, Rob Leurs

Publikováno v: The Journal of Pharmacology and Experimental Therapeutics, 311(1), 131-8. American Society for Pharmacology and Experimental Therapeutics
Bakker, R A, Dees, G, Carrillo, J J, Booth, R G, Lopez-Gimenez, J F, Milligan, G, Strange, P G & Leurs, R 2004, ' Domain swapping in the human histamine H1 receptor ', The Journal of Pharmacology and Experimental Therapeutics, vol. 311, no. 1, pp. 131-8 . https://doi.org/10.1124/jpet.104.067041

G-protein-coupled receptors (GPCRs) represent the largest family of receptors involved in transmembrane signaling. Although these receptors were generally believed to be monomeric entities, accumulating evidence supports the presence of GPCRs in mult

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9bdc1d2960c1fe01d5abc5fb7536c726
https://doi.org/10.1124/jpet.104.067041