Structural insight into the stabilization of microtubules by taxanes

Autor: Wei-Shuo Fang, Shinji Kamimura, Juan F. Giménez-Abián, María A. Oliva, Yun-Tao Ma, Juan Estévez-Gallego, J. Fernando Díaz, Andrea E. Prota, Daniel Lucena-Agell, Katja Bargsten, Kenneth Goossens, Shuo Li, José Manuel Andreu, Natacha Olieric, Carlos Roca, Michel O. Steinmetz, Tobias Mühlethaler, Ángeles Canales, Fernando Josa-Prado, Karl-Heinz Altmann

Paclitaxel (Taxol®) is a taxane and a first-line chemotherapeutic drug that stabilizes microtubules. While the interaction of paclitaxel with microtubules is well described, the current lack of high-resolution structural information on a tubulin-tax

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::8bc20ec6d6b1ba8992558ceb43d8dba4
https://doi.org/10.1101/2021.07.20.453061

Cohesin is dispensable for centromere cohesion in human cells.

Autor: Laura A Díaz-Martínez, Juan F Giménez-Abián, Duncan J Clarke

Publikováno v: PLoS ONE, Vol 2, Iss 3, p e318 (2007)

Proper regulation of the cohesion at the centromeres of human chromosomes is essential for accurate genome transmission. Exactly how cohesion is maintained and is then dissolved in anaphase is not understood.We have investigated the role of the cohes

Externí odkaz: https://doaj.org/article/eb4637ab134a4aeea53de99e58952ec3

Crystal Structure of the Cyclostreptin-Tubulin Adduct: Implications for Tubulin Activation by Taxane-Site Ligands

Autor: María A. Oliva, Karl-Heinz Altmann, Erik J. Sorensen, Juan Estévez-Gallego, Andrea E. Prota, Juan F. Giménez-Abián, J. Fernando Díaz, April L. Risinger, Michel O. Steinmetz, Christopher D. Vanderwal, Tobias Mühlethaler, Enrique Calvo, Francisco de Asís Balaguer, Susan L. Mooberry

Publikováno v: Digital.CSIC. Repositorio Institucional del CSIC
instname
International Journal of Molecular Sciences, 20 (6)
International Journal of Molecular Sciences
Volume 20
Issue 6
Repisalud
Instituto de Salud Carlos III (ISCIII)
International Journal of Molecular Sciences, Vol 20, Iss 6, p 1392 (2019)

It has been proposed that one of the mechanisms of taxane-site ligand-mediated tubulin activation is modulation of the structure of a switch element (the M-loop) from a disordered form in dimeric tubulin to a folded helical structure in microtubules.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1f9cfd00b1f1ee769e381ac20f964e74
http://hdl.handle.net/10261/178957

Analyzing Mitotic Chromosome Structural Defects After Topoisomerase II Inhibition or Mutation

Autor: Juan F, Giménez-Abián, Andrew B, Lane, Duncan J, Clarke

Publikováno v: Methods in molecular biology (Clifton, N.J.). 1703

For analyzing chromosome structural defects that result from topoisomerase II (topo II) dysfunction we have adapted classical cell cycle experiments, classical cytological techniques and the use of a potent topo II inhibitor (ICRF-193). In this chapt

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::92a67910365a94b0bae58dfc42cd474d
https://pubmed.ncbi.nlm.nih.gov/29177744

A novel chromatin tether domain controls topoisomerase IIα dynamics and mitotic chromosome formation

Autor: Andrew B. Lane, Duncan J. Clarke, Juan F. Giménez-Abián

Publikováno v: The Journal of Cell Biology

The dynamics of topoisomerase IIα binding to DNA and histones are important for successful mitosis and are regulated by a novel chromatin tether (ChT) domain in topoisomerase IIα.
DNA topoisomerase IIα (Topo IIα) is the target of an importan

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::db896bacf0396780d0009f17839c2728
https://doi.org/10.1083/jcb.201303045