p53-Independent checkpoint controls in a plant cell model

Autor: HELVIA R PELAYO, JUANA PINCHEIRA, JUAN F GIMÉNEZ-ABIÁN, DUNCAN J CLARKE, CONSUELO DE LA TORRE

Publikováno v: Biological Research, Vol 36, Iss 3-4, Pp 381-388 (2003)

Allium cepa L. meristems were used as a plant model to study the p53-independent control of S and G2 phases by checkpoint pathways, in eukaryotic cells. Checkpoint blocks were induced at early and mid S by hydroxyurea. After their spontaneous overrid

Externí odkaz: https://doaj.org/article/69606b0cbd134f3eb2763354ccacdfe0

PIASgamma is required for faithful chromosome segregation in human cells.

Autor: Laura A Díaz-Martínez, Juan F Giménez-Abián, Yoshiaki Azuma, Vincent Guacci, Gonzalo Giménez-Martín, Lorene M Lanier, Duncan J Clarke

Publikováno v: PLoS ONE, Vol 1, p e53 (2006)

BACKGROUND:The precision of the metaphase-anaphase transition ensures stable genetic inheritance. The spindle checkpoint blocks anaphase onset until the last chromosome biorients at metaphase plate, then the bonds between sister chromatids are remove

Externí odkaz: https://doaj.org/article/2bb829e6407144e9aa551e5bb6d27c83

Crystal Structure of the Cyclostreptin-Tubulin Adduct: Implications for Tubulin Activation by Taxane-Site Ligands

Autor: María A. Oliva, Karl-Heinz Altmann, Erik J. Sorensen, Juan Estévez-Gallego, Andrea E. Prota, Juan F. Giménez-Abián, J. Fernando Díaz, April L. Risinger, Michel O. Steinmetz, Christopher D. Vanderwal, Tobias Mühlethaler, Enrique Calvo, Francisco de Asís Balaguer, Susan L. Mooberry

Publikováno v: Digital.CSIC. Repositorio Institucional del CSIC
instname
International Journal of Molecular Sciences, 20 (6)
International Journal of Molecular Sciences
Volume 20
Issue 6
Repisalud
Instituto de Salud Carlos III (ISCIII)
International Journal of Molecular Sciences, Vol 20, Iss 6, p 1392 (2019)

It has been proposed that one of the mechanisms of taxane-site ligand-mediated tubulin activation is modulation of the structure of a switch element (the M-loop) from a disordered form in dimeric tubulin to a folded helical structure in microtubules.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1f9cfd00b1f1ee769e381ac20f964e74
http://hdl.handle.net/10261/178957

Analyzing Mitotic Chromosome Structural Defects After Topoisomerase II Inhibition or Mutation

Autor: Juan F, Giménez-Abián, Andrew B, Lane, Duncan J, Clarke

Publikováno v: Methods in molecular biology (Clifton, N.J.). 1703

For analyzing chromosome structural defects that result from topoisomerase II (topo II) dysfunction we have adapted classical cell cycle experiments, classical cytological techniques and the use of a potent topo II inhibitor (ICRF-193). In this chapt

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::92a67910365a94b0bae58dfc42cd474d
https://pubmed.ncbi.nlm.nih.gov/29177744

A novel chromatin tether domain controls topoisomerase IIα dynamics and mitotic chromosome formation

Autor: Andrew B. Lane, Duncan J. Clarke, Juan F. Giménez-Abián

Publikováno v: The Journal of Cell Biology

The dynamics of topoisomerase IIα binding to DNA and histones are important for successful mitosis and are regulated by a novel chromatin tether (ChT) domain in topoisomerase IIα.
DNA topoisomerase IIα (Topo IIα) is the target of an importan

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::db896bacf0396780d0009f17839c2728
https://doi.org/10.1083/jcb.201303045