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pro vyhledávání: '"Juan B. Villalobos-González"'
Autor:
Marcos Mancilla, Maritza Grandón, José Saavedra, Patricio Bustos, Harry Bohle, Juan B. Villalobos-González
Publikováno v:
Frontiers in Microbiology, Vol 9 (2018)
Antibiotic resistance is a major public health concern due to its association with the loss of efficacy of antimicrobial therapies. Horizontal transfer events may play a significant role in the dissemination of resistant bacterial phenotypes, being m
A simple solution for antibody signal enhancement in immunofluorescence and triple immunogold assays
Autor:
Felipe A. Beltrán, Abraham Rosas-Arellano, Juan B. Villalobos-González, Fanis Missirlis, Maite A. Castro, Lourdes Palma-Tirado, Alfonso Cárabez-Trejo
Publikováno v:
Histochemistry and cell biology. 146(4)
Immunolocalization techniques are standard in biomedical research. Tissue fixation with aldehydes and cell membrane permeabilization with detergents can distort the specific binding of antibodies to their high affinity epitopes. In immunofluorescence