Zobrazeno 1 - 10 of 15 pro vyhledávání: '"Juan A. Hernandez Bort"'
1
Akademický článek
Crucial aspects for maintaining rAAV stability
Autor: Johannes Lengler, Mia Gavrila, Janina Brandis, Kristina Palavra, Felix Dieringer, Sabine Unterthurner, Felix Fuchsberger, Barbara Kraus, Juan A. Hernandez Bort
Publikováno v: Scientific Reports, Vol 14, Iss 1, Pp 1-13 (2024)
Abstract The storage of rAAV vectors for gene therapy applications is critical for ensuring a constant product quality and defined amount of medication at the time of administration. Therefore, we determined the influence of different storage conditi
Externí odkaz: https://doaj.org/article/4272587a67954f3d829932e48dd955f5
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2
Akademický článek
Assessment of the percentage of full recombinant adeno-associated virus particles in a gene therapy drug using CryoTEM
Autor: Mathieu Colomb-Delsuc, Roman Raim, Christian Fiedler, Stefan Reuberger, Johannes Lengler, Rickard Nordström, Martin Ryner, Ioana Mihaela Folea, Barbara Kraus, Juan A. Hernandez Bort, Ida-Maria Sintorn
Publikováno v: PLoS ONE, Vol 17, Iss 6 (2022)
In spite of continuous development of gene therapy vectors with thousands of drug candidates in clinical drug trials there are only a small number approved on the market today stressing the need to have characterization methods to assist in the valid
Externí odkaz: https://doaj.org/article/ef333d3a4b1c483fa17c63bba0d3cba0
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5
Proton-transfer-reaction mass spectrometry (PTR-MS) for online monitoring of glucose depletion and cell concentrations in HEK 293 gene therapy processes
Autor: Mark Duerkop, Barbara Kraus, Johanna Jahn, Gerald Striedner, Benjamin Bayer, Ewald Kapeller, Andreas Maccani, Juan A Hernandez Bort, Robert Pletzenauer
Publikováno v: Biotechnology letters. 44(1)
Objectives The applicability of proton-transfer-reaction mass spectrometry (PTR-MS) as a versatile online monitoring tool to increase consistency and robustness for recombinant adeno-associated virus (rAAV) producing HEK 293 bioprocesses was evaluate
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b6bf1678d51d6246ee7b055d5bc7b559
https://pubmed.ncbi.nlm.nih.gov/34767126
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6
A metabolic CRISPR-Cas9 screen in Chinese hamster ovary cells identifies glutamine-sensitive genes
Autor: Lasse Ebdrup Pedersen, Karen Julie la Cour Karottki, Helene Faustrup Kildegaard, Alex Thomas, Hooman Hefzi, Nathan E. Lewis, Songyuan Li, Chintan Joshi, Philipp Spahn, Juan A. Hernandez Bort, Gyun Min Lee, David E. Ruckerbauer, Nicole Borth, Jae Seong Lee
Publikováno v: Metab Eng
Karottki, K J L C, Hefzi, H, Li, S, Pedersen, L E, Spahn, P N, Joshi, C, Ruckerbauer, D, Bort, J A H, Thomas, A, Lee, J S, Borth, N, Lee, G M, Kildegaard, H F & Lewis, N E 2021, ' A metabolic CRISPR-Cas9 screen in Chinese hamster ovary cells identifies glutamine-sensitive genes ', Metabolic Engineering, vol. 66, pp. 114-122 . https://doi.org/10.1016/j.ymben.2021.03.017
Over the past decades, optimization of media formulation and feeding strategies have fueled a many-fold improvement in CHO-based biopharmaceutical production. While Design of Experiments (DOE) and media screens have led to many advances, genome editi
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::55d3a308807be59b6b5c1d42a0a54b70
https://pubmed.ncbi.nlm.nih.gov/33813034
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7
A screening method to assess biological effects of microRNA overexpression in Chinese hamster ovary cells
Autor: Matthias Wieser, Matthias Hackl, Juan A. Hernandez Bort, Nicole Borth, Vaibhav Jadhav, Johannes Grillari, Eva Harreither, Renate Kunert
Publikováno v: Biotechnology and Bioengineering. 109:1376-1385
MicroRNAs (miRNAs) are a novel class of short non-coding RNAs, which negatively regulate target gene expression at post-transcriptional level. They mediate an important layer of control in the global regulation of gene networks, controlling a broad r
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d1728ef1aed9cde07aee12f0fdf805d
https://doi.org/10.1002/bit.24490
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8
Utilization and evaluation of CHO-specific sequence databases for mass spectrometry based proteomics
Autor: Oliver Rupp, Shane Kelly, Michael Henry, Thomas Noll, Paula Meleady, Colin Clarke, Matthias Hackl, Juan A. Hernandez Bort, Johannes Grillari, Raimund Hoffrogge, Martin Clynes, Nicole Borth, Niall Barron, Alf Pühler, Tim F Beckmann, Karina Brinkrolf, Padraig Doolan, Benjamin Müller
Publikováno v: Biotechnology and Bioengineering. 109:1386-1394
Recently released sequence information on Chinese hamster ovary (CHO) cells promises to not only facilitate our understanding of these industrially important cell factories through direct analysis of the sequence, but also to enhance existing methodo
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5ca6b8f154692af2ad3ccff90c0e3bd5
https://doi.org/10.1002/bit.24476
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9
Dynamic mRNA and miRNA profiling of CHO-K1 suspension cell cultures
Autor: Wolfgang Ernst, Helga Höflmayer, Eva Harreither, Nicole Borth, Vaibhav Jadhav, Niraj Kumar, Juan A. Hernandez Bort, Matthias Hackl, Johannes Grillari
Publikováno v: Biotechnology Journal. 7:500-515
In spite of the importance of Chinese hamster ovary (CHO) cells for recombinant protein production, very little is known about the molecular and gene regulatory mechanisms that control cellular phenotypes such as enhanced growth under serum-free cond
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::521021179289eef2b1feeb54695e4934
https://doi.org/10.1002/biot.201100143
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