Zobrazeno 1 - 10
of 36
pro vyhledávání: '"Juan‐Carlos Sigala"'
Publikováno v:
mLife, Vol 2, Iss 1, Pp 101-104 (2023)
Externí odkaz:
https://doaj.org/article/5a1687bbfbc840019c56ae6508ce18ad
Autor:
Daniela Velazquez, Juan-Carlos Sigala, Luz María Martínez, Paul Gaytán, Guillermo Gosset, Alvaro R. Lara
Publikováno v:
Microbial Cell Factories, Vol 21, Iss 1, Pp 1-14 (2022)
Abstract Background Fed-batch mode is the standard culture technology for industrial bioprocesses. Nevertheless, most of the early-stage cell and process development is carried out in batch cultures, which can bias the initial selection of expression
Externí odkaz:
https://doaj.org/article/586b3932eaae4e9d9138b51a794a4d69
Autor:
Mitzi de la Cruz, Flavio Kunert, Hilal Taymaz-Nikerel, Juan-Carlos Sigala, Guillermo Gosset, Jochen Büchs, Alvaro R. Lara
Publikováno v:
Microorganisms, Vol 12, Iss 1, p 150 (2024)
The demand of plasmid DNA (pDNA) as a key element for gene therapy products, as well as mRNA and DNA vaccines, is increasing together with the need for more efficient production processes. An engineered E. coli strain lacking the phosphotransferase s
Externí odkaz:
https://doaj.org/article/85225fdfb16a458787de813dd9d8e949
Publikováno v:
Journal of Biological Engineering, Vol 11, Iss 1, Pp 1-9 (2017)
Abstract Oxygen-responsive promoters can be useful for synthetic biology applications, however, information on their characteristics is still limited. Here, we characterized a group of heterologous microaerobic globin promoters in Escherichia coli. G
Externí odkaz:
https://doaj.org/article/dd2172ad511a47808c661b33fabe5669
Autor:
Karim E. Jaén, Juan-Carlos Sigala, Roberto Olivares-Hernández, Karsten Niehaus, Alvaro R. Lara
Publikováno v:
BMC Biotechnology, Vol 17, Iss 1, Pp 1-12 (2017)
Abstract Background Dissolved oxygen tension (DOT) is hardly constant and homogenously distributed in a bioreactor, which can have a negative impact in the metabolism and product synthesis. However, the effects of DOT on plasmid DNA (pDNA) production
Externí odkaz:
https://doaj.org/article/1a0b90feaef84d498332d2fb931778c9
Publikováno v:
Microorganisms, Vol 8, Iss 9, p 1444 (2020)
The design of optimal cell factories requires engineering resource allocation for maximizing product synthesis. A recently developed method to maximize the saving in cell resources released 0.5% of the proteome of Escherichia coli by deleting only th
Externí odkaz:
https://doaj.org/article/82b1af9f12bb4585b022e7598b0d1897
Akademický článek
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Publikováno v:
Process Biochemistry. 106:130-136
A novel microaerobically inducible pUC18-derived plasmid was produced in Escherichia coli chromosomally expressing Vitreoscilla hemoglobin. The plasmid contains a copy of the positive replication control molecule rnaII under the control of the Pvgb p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d2df7f67e3abf32ce64b31011bf93c9f
https://doi.org/10.1016/j.procbio.2021.04.013
https://doi.org/10.1016/j.procbio.2021.04.013
Publikováno v:
Journal of Microbiology and Biotechnology. 30:1592-1596
The aerobic growth and metabolic performance of Escherichia coli strains BL21 and W3110 were studied when the Vitreoscilla hemoglobin (VHb) was constitutively expressed in the chromosome. When VHb was expressed, acetate production decreased in both s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f26dc5fd789b40ba4be1019eab12c1f4
https://doi.org/10.4014/jmb.2004.04030
https://doi.org/10.4014/jmb.2004.04030
Publikováno v:
Applied Microbiology and Biotechnology. 103:6217-6229
Acinetobacter baylyi ADP1 is a microorganism with the potential to produce storage lipids. Here, a systematic study was carried out to evaluate growth performance and accumulation of wax esters and triacylglycerols using glycerol, xylose, glucose, ac
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae4e2d197527a465749237cdef5956d0
https://doi.org/10.1007/s00253-019-09910-z
https://doi.org/10.1007/s00253-019-09910-z