Zobrazeno 1 - 7 of 7 pro vyhledávání: '"Ju. A. Gladilina"'
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Construction and Characterization of a Recombinant Mutant Homolog of the CheW Protein from Thermotoga petrophila RKU-1
Autor: Nikolay N. Sokolov, A. L. Milyushkina, D. V. Grishin, O. V. Podobed, M. A. Vigovskiy, D. D. Zhdanov, M. V. Pokrovskaya, Ju. A. Gladilina, Vadim S. Pokrovsky, S. S. Aleksandrova
Publikováno v: Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry. 12:143-150
In the work a recombinant chemotaxis protein CheW from Thermotoga petrophila RKU-1 (TpeCheW) and its mutant homolog (TpeCheW-mut) were created. It was shown that, despite the low homology with CheW prototypes from intestinal bacteria, these proteins
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::65dcfddfd374ed288f6a710c0b3733f9
https://doi.org/10.1134/s1990750818020051
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4
Prediction of protein thermostability from their primary structure: the current state and development factors
Autor: D. V. Grishin, M. V. Pokrovskaya, O. V. Podobed, Nikolay N. Sokolov, S. S. Aleksandrova, Vadim S. Pokrovsky, Ju. A. Gladilina
Publikováno v: Biomeditsinskaya Khimiya. 63:124-131
The construction of proteins and peptides with desired properties, including resistance to high temperatures, as well as optimization of their amino acid composition, is an important and complex task, which attracts much attention in various branches
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f8518732c7656090baed4705f80125d7
https://doi.org/10.18097/pbmc20176302124
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5
[Preparation and characterization of a new mutant homolog of chemotaxis protein CheY from anaerobic hyperthermophilic microorganism Thermotoga naphthophila]
Autor: D. V. Grishin, Nikolay N. Sokolov, S. S. Aleksandrova, I. Yu. Toropygin, D. D. Zhdanov, V. S. Pokrovskiy, Ju. A. Gladilina, M. V. Pokrovskaya
Publikováno v: Biomeditsinskaia khimiia. 65(1)
Using genetic engineering methods the expression vectors structures have been designed to produce recombinant proteins TnaCheY and Tna CheY-mut, the homologues of the chemotaxis protein CheY from the hyperthermophilic organism Thermotoga naphthophila
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7ec2d307d19f8ce350e2e1d49235986a
https://pubmed.ncbi.nlm.nih.gov/30816096
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6
[Suppression of telomerase activity leukemic cells by mutant forms of Rhodospirillum rubrum L-asparaginase]
Autor: M V, Pokrovskaya, D D, Zhdanov, M A, Eldarov, S S, Aleksandrova, A V, Veselovskiy, V S, Pokrovskiy, D V, Grishin, Ju A, Gladilina, N N, Sokolov
Publikováno v: Biomeditsinskaia khimiia. 63(1)
The active and stable mutant forms of short chain cytoplasmic L-asparaginase type I of Rhodospirillum rubrum (RrA): RrA+N17, D60K, F61L, RrA+N17, A64V, E67K, RrA+N17, E149R, V150P, RrAE149R, V150P and RrAE149R, V150P, F151T were obtained by the metho
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::41b2262040c2391a60b68d2760aba5e1
https://pubmed.ncbi.nlm.nih.gov/28251953
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7
Creation of a producent, optimization of expression, and purification of recombinant Yersinia pseudotuberculosis L-asparaginase
Autor: K. V. Sidoruk, Ju. A. Gladilina, N. M. Omeljanuk, Vadim S. Pokrovsky, S. S. Aleksandrova, Nikolay N. Sokolov, M. V. Pokrovskaya, A. A. Borisova, V. G. Bogush
Publikováno v: ResearcherID
Recombinant E. coli strain producing Y. pseudotuberculosis Q66CJ2 (YpA) L-asparaginase II was created. Gene ansB homologue encoding Y. pseudotuberculosis IP 32953 L-asparaginase precursor was synthesized. The gene was cloned in pBad24 expression vect
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8268166519975ecb219aac9be855eeb1
https://pubmed.ncbi.nlm.nih.gov/22808465
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