Zobrazeno 1 - 3
of 3
pro vyhledávání: '"Jozsef Vasi"'
Publikováno v:
Journal of Chromatography A. 1057:115-124
An integrated process for purifying a 6.1 kilo base pair (kbp) plasmid from a clarified Escherichia coli cell lysate based on an ultra/diafiltration step combined with polymer/polymer aqueous two-phase system and a new type of chromatography is descr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4a980ff14bc6a6f15c805876ac4cf7fa
https://doi.org/10.1016/j.chroma.2004.09.060
https://doi.org/10.1016/j.chroma.2004.09.060
Publikováno v:
Journal of Biotechnology. 109:193-199
Supercoiled plasmid DNA was selectively purified from its open circular form by thiophilic interaction chromatography, performed in the presence of high concentrations of water-structuring salts. To identify optimal conditions for purification, vario
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::881241333f1afbbf0c4387eb4c1bd3d6
https://doi.org/10.1016/j.jbiotec.2003.10.036
https://doi.org/10.1016/j.jbiotec.2003.10.036
Autor:
Marianne Sparrman, Lena M. Sandberg, Jozsef Vasi, Per Anders Nilsson, Raf Lemmens, Emma Håkansson, Annika Andersson
Publikováno v:
Molecular Therapy. 9:S312-S313
The production of highly purified supercoiled plasmid DNA in large quantities for gene therapy is a prerequisit for the success of plasmid DNA as a therapeutic. The shift from small-scale plasmid production (for cell transfections at the benchtop lev
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e075e118deb68f0b3509d6135399489f
https://doi.org/10.1016/j.ymthe.2004.06.722
https://doi.org/10.1016/j.ymthe.2004.06.722