Zobrazeno 1 - 10
of 25
pro vyhledávání: '"Jozef Kusba"'
Publikováno v:
Biopolymers. 70:595-603
Resonance energy transfer (RET) is typically limited to distances below 60 A, which can be too short for some biomedical assays. We examined a new method for increasing the RET distances by placing donor- and acceptor-labeled DNA oligomers between tw
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d80dbf7edc40074b0a4f0fca4b75219c
https://doi.org/10.1002/bip.10507
https://doi.org/10.1002/bip.10507
Publikováno v:
Analytical Biochemistry. 315:160-169
We examined the effects of metallic silver particles on resonance energy transfer (RET) between fluorophores covalently bound to DNA. A coumarin donor and a Cy3 acceptor were positioned at opposite ends of a 23-bp double helical DNA oligomer. In the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::646156406f57df0ff4faaa3e1da49a5c
https://doi.org/10.1016/s0003-2697(02)00710-8
https://doi.org/10.1016/s0003-2697(02)00710-8
Publikováno v:
Journal of Fluorescence. 12:439-447
We examined the photostability of a double-stranded DNA oligomer, covalently labeled with Cy3 or Cy5 on one strand, in the presence of metallic silver island films. In our experimental configuration a minor fraction of the labeled DNA was close to th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2a8c3c9175daea3afd5b52e631617208
https://doi.org/10.1023/a:1021370111590
https://doi.org/10.1023/a:1021370111590
Publikováno v:
Scopus-Elsevier
We describe experimental observations of fluorescence quenching by time-delayed light pulses whose wavelength overlaps the emission spectrum of 4-(dimethylamino)-4′-cyanostilbene (DCS). The relative cross sections for light quenching were proportio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff88a2f43d8cbbbc36597d782a799105
https://doi.org/10.1021/j100087a012
https://doi.org/10.1021/j100087a012
Autor:
Henryk Szmacinski, Wieslaw Wiczk, Joseph R. Lakowicz, Jozef Kusba, Ignacy Gryczynski, Gabor Laczko, Michael L. Johnson
Publikováno v:
Journal of Fluorescence. 1:163-176
We used gigahertz frequency-domain fluorometry to examine the tyrosyl fluorescence intensity and anisotropy decays of the single-tyrosine cyclic peptide hormones oxytocin and vasopressin. Acrylamide quenching and a distance-dependent quenching model
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b07207cbcaf9be1d424a09f747ca5554
https://doi.org/10.1007/bf00865363
https://doi.org/10.1007/bf00865363
Autor:
Joseph R. Lakowicz, Ewald Terpetschnig, Jozef Kusba, Henryk Malak, Michael L. Johnson, Ignacy Gryczynski, Engin U. Akkaya, Henryk Szmacinski
Publikováno v:
SPIE Proceedings.
The Center for Fluorescence Spectroscopy (CFS) is a multi-user facility providing state of the art time-resolved fluorescence instrumentation and software for scientists, whose research can be enhanced by such experimental data. The CFS is a national
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9eaea0d6bffde2b1e11bd0d0b5d87aa0
https://doi.org/10.1117/12.182725
https://doi.org/10.1117/12.182725
Publikováno v:
SPIE Proceedings.
We describe a method to improve the resolution of donor-to- acceptor distance distributions in molecules which are flexing on the timescale of the fluorescence lifetime. We measured the time- dependent donor decays of two donor (D)-acceptor (A) pairs
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::895cb06b15640707131c51606e4aa316
https://doi.org/10.1117/12.182763
https://doi.org/10.1117/12.182763
Publikováno v:
SPIE Proceedings.
Frequency-domain fluorometry was used to investigate the time- dependent intensity decays of N-acetyl-L-tryptophanamide (NATA) when collisionally quenched by acrylamide and iodide in propylene glycol at 20 degree(s)C. The intensity decays of NATA bec
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b26c754abaade73d270b6e40e4931796
https://doi.org/10.1117/12.182750
https://doi.org/10.1117/12.182750
Publikováno v:
SPIE Proceedings.
Recent experimental results from this laboratory have shown that fluorescence emission can be quenched by the light pulses from cavity-dumped dye lasers, a phenomenon we call `light quenching.' In this overview article we describe some of the possibl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1b48ef3feca3b8ca34eba071c8f272b2
https://doi.org/10.1117/12.182784
https://doi.org/10.1117/12.182784
Publikováno v:
SPIE Proceedings.
We have recently demonstrated that fluorescence emission can be quenched by laser light pulses from modern high-repetition rate lasers, a phenomenon we call 'light quenching'. We now describe some of the possible effects of light quenching on the tim
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3070227239cabc2b066bf1ee09dac40b
https://doi.org/10.1117/12.182737
https://doi.org/10.1117/12.182737