Výsledky vyhledávání - "Joyce A.M. Wootton"

Canine COL1A2 Mutation Resulting in C‐Terminal Truncation of Pro‐α2(I) and Severe Osteogenesis Imperfecta

Autor: James N. MacLeod, Ronald R. Minor, Bonnie G. Campbell, Joyce A.M. Wootton

Publikováno v: Journal of Bone and Mineral Research. 16:1147-1153

RNA and type I collagen were analyzed from cultured skin fibroblasts of a Beagle puppy with fractures consistent with type III osteogenesis imperfecta (OI). In a nonisotopic RNAse cleavage assay (NIRCA), the proband's RNA had a unique cleavage patter

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::505973f43cccad4ae4e2489feb61b89f
https://doi.org/10.1359/jbmr.2001.16.6.1147

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Sequence of Canine COL1A2 cDNA: Nucleotide Substitutions Affecting the Cyanogen Bromide Peptide Map of the α2(I) Chain

Autor: James N. MacLeod, Bonnie G. Campbell, Joyce A.M. Wootton, Ronald R. Minor

Publikováno v: Archives of Biochemistry and Biophysics. 357:67-75

The alpha2 chain of canine type I collagen was characterized with both sequence analysis of COL1A2 cDNA and chemical analysis of alpha2(I) chains. The complete sequence of canine COL1A2 cDNA was determined from reverse-transcribed and polymerase chai

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::03cd793faaabbe71105abf7c17617a2d
https://doi.org/10.1006/abbi.1998.0774

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Acute synovitis and intra-articular methylprednisolone acetate in ponies

Autor: Rory J. Todhunter, Susan L. Fubini, Joyce A.M. Wootton, Margaret Vernier-Singer, Kathleen P. Freeman, George Lust, James N. MacLeod

Publikováno v: Osteoarthritis and Cartilage. 6:94-105

Objective: To determine how acute synovitis, with and without intra-articular methylprednisolone acetate (MPA), affect synthesis of proteoglycan, total protein, and collagen in articular cartilage and total protein synthesis in synovial membrane. Des

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4fe453a37d9eaf926d81cced0f9de137
https://doi.org/10.1053/joca.1997.0099

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Cross-Validation of Cyanogen Bromide-Peptide Ratios to Measure the Proportion of Type II Collagen in Pepsin Digests of Equine Articular Cartilage, Meniscus, and Cartilage Repair Tissue

Autor: Rory J. Todhunter, Joyce A.M. Wootton, Ronald R. Minor, Naomi Altman, George Lust

Publikováno v: Analytical Biochemistry. 216:195-204

Collagen type I and type II were purified from equine flexor tendon and articular cartilage, respectively. Equal amounts of these collagens were cleaved with cyanogen bromide, and 11 mixtures containing increasing proportions of type II collagen were

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fd07541dc91f4a42f6edaeeb85d913e3
https://doi.org/10.1006/abio.1994.1025

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Effects of exercise and polysulfated glycosaminoglycan on repair of articular cartilage defects in the equine carpus

Autor: George Lust, Rory J. Todhunter, L. Krook, Joyce A.M. Wootton, Ronald R. Minor, Nancy Burton-Wurster

Publikováno v: Journal of Orthopaedic Research. 11:782-795

Our aim was to determine if mild to moderate postoperative exercise and intra-articular polysulfated glycosaminoglycan result in improved repair of large, experimentally induced osteochondral defects in a weight-bearing surface of equine joints. Arth

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e3978c120bdc780e8addbe6cd8dccc02
https://doi.org/10.1002/jor.1100110603

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Cloning and sequencing of the canine and feline cardiac troponin I genes

Autor: Joyce A.M. Wootton, Mark Rishniw, Stephen C. Barr, K. W. Simpson, Nena J. Winand

Publikováno v: American journal of veterinary research. 65(1)

Objective—To determine the gene sequences of canine and feline cardiac troponin I (cTnI), express the protein from the cloned gene in vitro, and validate the use of a commercial cTnI serum analyzer in these species via detection of the expressed pr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d3ca1d48c7b01d478599903c1eebebcc
https://pubmed.ncbi.nlm.nih.gov/14719702

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Sequence of normal canine COL1A1 cDNA and identification of a heterozygous alpha1(I) collagen Gly208Ala mutation in a severe case of canine osteogenesis imperfecta

Autor: Joyce A.M. Wootton, Ronald R. Minor, Bonnie G. Campbell, James N. MacLeod

Publikováno v: Archives of biochemistry and biophysics. 384(1)

The sequence of canine COL1A1 cDNA was determined from four overlapping COL1A1 RT-PCR products generated from canine fibroblast RNA. In the translated region, nucleotide identity between canine and human COL1A1 cDNA was 93.2%, although the canine seq

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8de0f9992790c6103823b6416d1bf91e
https://pubmed.ncbi.nlm.nih.gov/11147834

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[26] Detecting radiolabeled lipid-modified proteins in polyacrylamide gels

Autor: Jacqueline Debony, Anthony I. Magee, Joyce A.M. Wootton

Publisher Summary This chapter discusses the methods for labeling cells and detecting tritium-labeled proteins after sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE. The methods are specifically designed for use with fatty acid-mo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::e1675e557b8f02635af60912d2b5a6fd
https://doi.org/10.1016/0076-6879(95)50082-0

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