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Autor: David P. Lane, Farid J. Ghadessy, Ban Xiong Tan, Cynthia R. Coffill, Yaw Sing Tan, Hoe Peng Liew, Joy S. Chua

Publikováno v: Journal of Molecular Cell Biology

Mouse double minute (Mdm) genes span an evolutionary timeframe from the ancient eukaryotic placozoa Trichoplax adhaerens to Homo sapiens, implying a significant and possibly conserved cellular role throughout history. Maintenance of DNA integrity and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1fad52aa087bfdabe9280d3953430d82
https://doi.org/10.1093/jmcb/mjx002

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A novel molecular rotor facilitates detection of p53-DNA interactions using the Fluorescent Intercalator Displacement Assay

Autor: Farid J. Ghadessy, Ting Xiang Lim, Joy S. Chua, Walter Goh, Yin Nah Teo, Sydney Brenner, Min Yen Lee

Publikováno v: Scientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
Scientific Reports

We have investigated the use of fluorescent molecular rotors as probes for detection of p53 binding to DNA. These are a class of fluorophores that undergo twisted intramolecular charge transfer (TICT). They are non-fluorescent in a freely rotating co

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a166c07f50d2593a4352fce90b99a58a
https://doi.org/10.1038/s41598-018-31197-9

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Tumor-specific signaling to p53 is mimicked by Mdm2 inactivation in zebrafish: insights from mdm2 and mdm4 mutant zebrafish

Autor: David P. Lane, L Guo, Hoe Peng Liew, Joy S. Chua

Publikováno v: Oncogene

In mice, the deletion of either Mdm2 or Mdm4 results in a p53-dependent embryonic lethality. We used zinc-finger nucleases to construct mutations in the mdm2 and mdm4 genes of zebrafish. Although the loss of mdm2 results in a p53-dependent early embr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e9c0258d154a363b15822285778d4f1e
https://doi.org/10.1038/onc.2015.57

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SUMO-targeted ubiquitin E3 ligase RNF4 is required for the response of human cells to DNA damage

Autor: Joy S. Chua, Anne Seifert, Ronald T. Hay, Filip Golebiowski, Jean-François Maure, Yili Yin

Publikováno v: Genesdevelopment. 26(11)

Here we demonstrate that RNF4, a highly conserved small ubiquitin-like modifier (SUMO)-targeted ubiquitin E3 ligase, plays a critical role in the response of mammalian cells to DNA damage. Human cells in which RNF4 expression was ablated by siRNA or

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0c9b40761d77a99a9db450cf58464e0a
https://pubmed.ncbi.nlm.nih.gov/22661230

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Detection of the 113p53 protein isoform: a p53-induced protein that feeds back on the p53 pathway to modulate the p53 response in zebrafish

Autor: David Coomber, Kian Chung Lee, David P. Lane, Joy S. Chua, Danesh Vijayakumar, Hui-Yan Eng, Kathy Lim, Lin Guo, Farid J. Ghadessy

Publikováno v: Cell cycle (Georgetown, Tex.). 9(10)

Isoforms of p53 have been described in both zebrafish and human systems based on sensitive analysis of RNA using PCR-based methods. Despite consistent evidence of the existence of these isoforms at the RNA level it has been difficult to detect the en

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f394fa8a0438b93f15cbea29885f494
https://pubmed.ncbi.nlm.nih.gov/20505327

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