Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Joy L. Brooks"'
Publikováno v:
Microbiology. 144:3027-3037
SUMMARY: The porA gene from Neisseria meningitidis was cloned into the pRSETA vector and recombinant class 1 outer-membrane protein expressed at high levels in Escherichia coli. The protein was readily purified by affinity chromatography on a Ni2+ ma
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7ea079440d7e6265a56ccbedeba99d1e
https://doi.org/10.1099/00221287-144-11-3027
https://doi.org/10.1099/00221287-144-11-3027
Autor:
R.G. Kroll, Joy L. Brooks
Publikováno v:
Journal of Microbiological Methods. 16:125-137
Enzyme linked electrical detection systems were developed using a Listeria specific 16S rRNA directed oligonucleotide probe and species specific probes against polymerase chain reaction products specifically amplified from rDNA sequences of Carnobact
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ee5d2bd2c720fc1107a24ac59f9916b8
https://doi.org/10.1016/0167-7012(92)90032-y
https://doi.org/10.1016/0167-7012(92)90032-y
Publikováno v:
Journal of Immunological Methods. 140:79-84
A modification to the enzyme amplification system described by Self (1984), has been developed in which the addition of semicarbazide hydrochloride increased the sensitivity of detection of protein A-bearing Staphylococcus aureus in foods from 4–6
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aad54581ce1d9a4ae34736fbcfa3f506
https://doi.org/10.1016/0022-1759(91)90128-3
https://doi.org/10.1016/0022-1759(91)90128-3
Publikováno v:
Letters in Applied Microbiology. 11:119-122
An amperometric immunosensor specific to the protein A of Staphylococcus aureus, was developed using the direct electrochemical detection of phenol produced by alkaline phosphatase from phenylphosphate. The immunosensor could detect protein A at 0.01
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::779b185022799df31e10a82eb7238398
https://doi.org/10.1111/j.1472-765x.1990.tb00138.x
https://doi.org/10.1111/j.1472-765x.1990.tb00138.x
Publikováno v:
Journal of Applied Bacteriology. 68:577-585
An amperometric enzyme-linked immunosensor was developed to detect and quantify levels of Staphylococcus aureus electrically in pure cultures and in foods. The assay was a modification of a 'sandwich' ELISA for the protein A of Staph. aureus, employi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::68f39e0bab4473418d9dab1b6d677975
https://doi.org/10.1111/j.1365-2672.1990.tb05223.x
https://doi.org/10.1111/j.1365-2672.1990.tb05223.x
Publikováno v:
Microbiology (Reading, England). 144
The porA gene from Neisseria meningitidis was cloned into the pRSETA vector and recombinant class 1 outer-membrane protein expressed at high levels in Escherichia coli. The protein was readily purified by affinity chromatography on a Ni2+ matrix and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::59a3616eeacdea1509a6b472c080c31b
https://pubmed.ncbi.nlm.nih.gov/9846738
https://pubmed.ncbi.nlm.nih.gov/9846738
Autor:
K. A. V. Cartwright, A. R. O. Miller, Graeme Jones, Joy L. Brooks, Myron Christodoulides, John E. Heckels
Publikováno v:
The Journal of infectious diseases. 178(2)
Meningococcal carriage and the immune response to colonization were studied in a group of military recruits undergoing basic training. Subtyping by determination of the class 1 protein sequence clearly differentiated between strains and demonstrated
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::693ec86e60b0398fbf1941a518ee1618
https://pubmed.ncbi.nlm.nih.gov/9697726
https://pubmed.ncbi.nlm.nih.gov/9697726
Publikováno v:
FEMS microbiology letters. 128(2)
The meningococcal parA gene encodes the class 1 outer membrane protein which contains the VR1 and VR2 regions responsible for sero-subtype specificity. However, sequence variations may occur within these regions which are not recognised by the curren
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::87ef97954a4abb460b36df816864124f
https://pubmed.ncbi.nlm.nih.gov/7750732
https://pubmed.ncbi.nlm.nih.gov/7750732
Publikováno v:
The Journal of applied bacteriology. 73(3)
Two enzyme-linked amperometric immunosensors specific for salmonellas were developed as rapid methods for quantifying and detecting these organisms in pure cultures and foods. Both used alkaline phosphatase as the enzyme reporter molecule but one sys
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eadffb50c0724c8d8721521cf23dcc31
https://pubmed.ncbi.nlm.nih.gov/1399912
https://pubmed.ncbi.nlm.nih.gov/1399912
Publikováno v:
The Journal of applied bacteriology. 72(4)
J.L. BROOKS, A.S. MOORE, R.A. PATCHETT, M. D. COLLINS AND R.G. KROLL. 1992. The polymerase chain reaction (PCR) was used selectively to amplify specific rDNA sequences of Carnobacterium divergens, C. mobile, C. piscicola and C. gallinarum in purified
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee8ed5533d502881334c8c1e437931a8
https://pubmed.ncbi.nlm.nih.gov/1381346
https://pubmed.ncbi.nlm.nih.gov/1381346