Výsledky vyhledávání - "Joy E Chamberlin"

Examination of the role of Gln-158 in the mechanism of CO(2) hydration catalyzed by beta-carbonic anhydrase from Arabidopsis thaliana

Autor: Roger S. Rowlett, Paul S Murray, Joy E Chamberlin, Chingkuang Tu

Publikováno v: Archives of biochemistry and biophysics. 425(1)

We have cloned and overexpressed a variant of Arabidopsis thaliana β-carbonic anhydrase (Q158A) that deletes the functional equivalent of the backbone amide NH of Thr-199 in human α-carbonic anhydrase II. The latter residue is hypothesized to be im

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9382fc99297b6fda5cacc35658fe0581
https://pubmed.ncbi.nlm.nih.gov/15081890

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Kinetic characterization of wild-type and proton transfer-impaired variants of beta-carbonic anhydrase from Arabidopsis thaliana

Autor: Melissa M McKay, Jeffrey R Preiss, Roger S. Rowlett, Joy E Chamberlin, Robb J Marchione, Jacob A Strong, Chingkuang Tu, George S Donovan, Katherine A Hicks, Rebecca J Loomis

Publikováno v: Archives of biochemistry and biophysics. 404(2)

We have cloned and overexpressed a truncated, recombinant form of β-carbonic anhydrase from Arabidopsis thaliana . The wild-type enzyme and two site-directed variants, H216N and Y212F, have been kinetically characterized both at steady state by stop

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::11540273fad8c397e4ee3972d752b100
https://pubmed.ncbi.nlm.nih.gov/12147257

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