Single-molecule FRET unveils induced-fit mechanism for substrate selectivity in flap endonuclease 1

Autor: Rashid, Fahad, Harris, Paul D, Zaher, Manal S, Sobhy, Mohamed A, Joudeh, Luay I, Yan, Chunli, Piwonski, Hubert, Tsutakawa, Susan E, Ivanov, Ivaylo, Tainer, John A, Habuchi, Satoshi, Hamdan, Samir M

Human flap endonuclease 1 (FEN1) and related structure-specific 5'nucleases precisely identify and incise aberrant DNA structures during replication, repair and recombination to avoid genomic instability. Yet, it is unclear how the 5'nuclease mechani

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https://escholarship.org/uc/item/5cb0g4cd

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Sequential and Multistep Substrate Interrogation Provides the Scaffold for Specificity in Human Flap Endonuclease 1

Autor: Sobhy, Mohamed A., Joudeh, Luay I., Huang, Xiaojuan, Takahashi, Masateru, Hamdan, Samir M.

Publikováno v: In Cell Reports 27 June 2013 3(6):1785-1794

Mechanistic investigation of human maturation of Okazaki fragments reveals slow kinetics

Autor: Vlad-Stefan Raducanu, Muhammad Tehseen, Amani Al-Amodi, Luay I. Joudeh, Alfredo De Biasio, Samir M. Hamdan

The final steps of lagging strand synthesis induce maturation of Okazaki fragments via removal of the RNA primers and ligation. Iterative cycles between Polymerase δ (Polδ) and Flap endonuclease-1 (FEN1) remove the primer, with an intermediary nick

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0bdea5a560e1f7c039d1962c60a4f8df
https://www.repository.cam.ac.uk/handle/1810/345575

Single-molecule FRET unveils induced-fit mechanism for substrate selectivity in flap endonuclease 1.

Autor: Rashid F; Division of Biological and Environmental Science and Engineering, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia., Harris PD; Division of Biological and Environmental Science and Engineering, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia., Zaher MS; Division of Biological and Environmental Science and Engineering, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia., Sobhy MA; Division of Biological and Environmental Science and Engineering, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia., Joudeh LI; Division of Biological and Environmental Science and Engineering, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia., Yan C; Department of Chemistry, Georgia State University, Atlanta, United States.; Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, United States., Piwonski H; Division of Biological and Environmental Science and Engineering, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia., Tsutakawa SE; Lawrence Berkeley National Laboratory, Berkeley, United States., Ivanov I; Department of Chemistry, Georgia State University, Atlanta, United States.; Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, United States., Tainer JA; Lawrence Berkeley National Laboratory, Berkeley, United States.; Department of Molecular and Cellular Oncology, University of Texas MD Anderson Cancer Center, Houston, United States., Habuchi S; Division of Biological and Environmental Science and Engineering, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia., Hamdan SM; Division of Biological and Environmental Science and Engineering, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.

Publikováno v: ELife [Elife] 2017 Feb 23; Vol. 6. Date of Electronic Publication: 2017 Feb 23.